Plant hormones coordinate monolignol biosynthesis with seasonal changes in Populus tomentosa

Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar

PeerJ ◽

10.7717/peerj.10741 ◽

2021 ◽

Vol 9 ◽

pp. e10741

Author(s):

Nan Chao ◽

Qi Qi ◽

Shuang Li ◽

Brent Ruan ◽

Xiangning Jiang ◽

...

Keyword(s):

Shikimic Acid ◽

Lignin Content ◽

Expression Profiles ◽

Populus Trichocarpa ◽

Functional Divergence ◽

Lignin Biosynthesis ◽

Populus Tomentosa ◽

Motif Analysis ◽

Monolignol Biosynthesis ◽

Populus Tomentosa Carr

Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.

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SEASONAL CHANGES OF ENDOGENOUS PLANT HORMONES IN ROSA CANINA

Acta Horticulturae ◽

10.17660/actahortic.2005.690.30 ◽

2005 ◽

pp. 199-202

Author(s):

I. Yörük ◽

M. Türker ◽

P. Battal ◽

A. Kazankaya ◽

B. Tileklioğlu

Keyword(s):

Plant Hormones ◽

Seasonal Changes ◽

Rosa Canina ◽

Endogenous Plant Hormones ◽

Endogenous Plant

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Seasonal changes and metabolism of plant hormones in root nodules ofLens sp.

Biologia Plantarum ◽

10.1007/bf02902904 ◽

1984 ◽

Vol 26 (4) ◽

pp. 253-259 ◽

Cited By ~ 8

Author(s):

T. K. Dangar ◽

P. S. Basu

Keyword(s):

Plant Hormones ◽

Seasonal Changes ◽

Root Nodules

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Novel motif is capable of determining CCR and CCR-like proteins based on the divergence of CCRs in plants

Tree Physiology ◽

10.1093/treephys/tpz098 ◽

2019 ◽

Vol 39 (12) ◽

pp. 2019-2026

Author(s):

Nan Chao ◽

Wen-Ting Jiang ◽

Xue-Chun Wang ◽

Xiang-Ning Jiang ◽

Ying Gai

Keyword(s):

Panicum Virgatum ◽

Coenzyme A ◽

Oryza Sativa L ◽

Populus Tomentosa ◽

Land Plants ◽

Binding Motif ◽

Monolignol Biosynthesis ◽

Panicum Virgatum L ◽

Populus Tomentosa Carr ◽

Selaginella Moellendorffii

Abstract Cinnamoyl-coenzyme A reductases (CCRs) have been reported as key enzymes involved in monolignol biosynthesis. In this study, a motif-aware workflow based on a new signature motif effectively distinguished CCRs from CCR-like proteins. The divergence of CCRs and CCR-like sequences in Populus tomentosa Carr, Panicum virgatum L, Oryza sativa L and Selaginella moellendorffii Hieron suggests that NWYCY is not efficient for CCR recognition. The novel motif H202(X)2K205 (CCR-SBM or CCR substrate binding motif) was introduced to distinguish between CCRs and CCR-like proteins. The site-directed mutant R205K in Os(I)CCR-like and H202 in PtoCCR7 resulted in the rescue and loss of activity, respectively, further validating the fact that CCR-SBM is critical for maintaining CCR activity. The molecular docking using feruloyl-cinnamoyl-coenzyme A (CoA) as the ligand and binary PhCCR-NADP structures as receptors indicated an interaction between H202 and K205 with CoA moiety. The genuine CCRs and CCR-like proteins from several angiosperms and gymnosperms were screened using a motif-aware workflow and were validated using a biochemical assay. Our results suggest that the motif-aware workflow is efficient and effective for the identification of CCRs and CCR-like proteins in land plants and can be used as a more accurate way of identifying genuine CCRs among land plants.

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Seasonal Changes in Endogenous Plant Hormones and Alternate Bearing of Nabali Olive (Olea europea L.)Trees

Asian Journal of Plant Sciences ◽

10.3923/ajps.2013.241.246 ◽

2013 ◽

Vol 12 (6-8) ◽

pp. 241-246

Author(s):

T. Abu-Zahra ◽

H. Al-Dmoor

Keyword(s):

Plant Hormones ◽

Seasonal Changes ◽

Alternate Bearing ◽

Endogenous Plant Hormones ◽

Olea Europea ◽

Endogenous Plant

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Spreading the Word: Seasonal Changes Mean New Opportunities for Media Coverage

ASHA Leader ◽

10.1044/leader.stw.18072013.np ◽

2013 ◽

Vol 18 (7) ◽

Keyword(s):

Media Coverage ◽

Seasonal Changes ◽

Swimming Pools

Summer kicking into high gear conjures images of swimming pools and barbeques. But before you book your beach house for the weekend, think about what the changing seasons can mean for you professionally.

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