Virus-Mediated Targeted DNA Methylation Illuminates the Dynamics of Methylation in an Endogenous Plant Gene

DNA methylation maintains genome stability and regulates gene expression in plants. RNA-directed DNA methylation (RdDM) is critical for appropriate methylation. However, no efficient tools are available for the investigation of the functions of specific DNA methylation. In this study, the cucumber mosaic virus vector was used for targeted DNA methylation. Methylation was rapidly induced but gradually decreased from the 3′ end of the target endogenous sequence in Nicotiana benthamiana, suggesting a mechanism to protect against the ectopic introduction of DNA methylation. Increasing 24-nt siRNAs blocked this reduction in methylation by down-regulating DCL2 and DCL4. RdDM relies on the sequence identity between RNA and genomic DNA; however, this identity does not appear to be the sole determinant for efficient DNA methylation. The current findings provide new insight into the regulation of DNA methylation and promote additional effort to develop efficient targeted DNA methylation in plants.

The Effect of Salicylic Acid and 20 Substituted Molecules on Alleviating Metolachlor Herbicide Injury in Rice (Oryza sativa)

Salicylic acid (SA) is an endogenous plant hormone that has a wide range of pharmacological effects. Studies have indicated that SA has herbicide safening activity. In this study, the herbicide safening activity of SA and 20 substituted molecules were tested on agar-cultured rice. Biological assay results indicated that SA and substituted SA had a low inhibitory effect on the growth of rice seedlings (Oryza sativa), and partially alleviated the effects of metolachlor toxicity. Moreover, at 0.25 mg L−1, the safening effect of compounds l and u lessened the effects of metolachlor phytotoxicity on plant height and fresh weight when compared to the effects of the control, fenclorim. The effects of metolachlor toxicity were reduced on root length due to the safening effects of compounds l, n, and u; these effects were greater than those of fenclorim. These compounds could facilitate the development of novel herbicide safeners.

The role of endogenous elicitor peptides in plant resistance to biotic stress

Plant elicitor peptides (Peps) are one class of elicitor substances, which are formed in plant cells in response to various biotic stressors and induced of nonspecific plant resistance. They are present and active in angiosperms, including many important agricultural crops, and can be considered as a promising class of compounds for creating environmentally safe drugs that induce phytoimmunity and increase the resistance of plants to stress. In this paper, an analysis of current literature data on the functional activity of endogenous plant elicitor peptides, the mechanisms of Pep-signaling and their role in plant resistance to biotic stresses is carried out.