Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs

2021 ◽  
pp. 112308
Author(s):  
Md Suruzzaman ◽  
Tengxin Cao ◽  
Jinping Lu ◽  
Yongjing Wang ◽  
Ming Su ◽  
...  
2012 ◽  
Vol 12 (5) ◽  
pp. 699-706 ◽  
Author(s):  
B. S. Marti ◽  
G. Bauser ◽  
F. Stauffer ◽  
U. Kuhlmann ◽  
H.-P. Kaiser ◽  
...  

Well field management in urban areas faces challenges such as pollution from old waste deposits and former industrial sites, pollution from chemical accidents along transport lines or in industry, or diffuse pollution from leaking sewers. One possibility to protect the drinking water of a well field is the maintenance of a hydraulic barrier between the potentially polluted and the clean water. An example is the Hardhof well field in Zurich, Switzerland. This paper presents the methodology for a simple and fast expert system (ES), applies it to the Hardhof well field, and compares its performance to the historical management method of the Hardhof well field. Although the ES is quite simplistic it considerably improves the water quality in the drinking water wells. The ES knowledge base is crucial for successful management application. Therefore, a periodic update of the knowledge base is suggested for the real-time application of the ES.


2021 ◽  
Vol 9 (4) ◽  
pp. 765
Author(s):  
Janika Wolff ◽  
Martin Beer ◽  
Bernd Hoffmann

Outbreaks of the three capripox virus species, namely lumpy skin disease virus, sheeppox virus, and goatpox virus, severely affect animal health and both national and international economies. Therefore, the World Organization for Animal Health (OIE) classified them as notifiable diseases. Until now, discrimination of capripox virus species was possible by using different conventional PCR protocols. However, more sophisticated probe-based real-time qPCR systems addressing this issue are, to our knowledge, still missing. In the present study, we developed several duplex qPCR assays consisting of different types of fluorescence-labelled probes that are highly sensitive and show a high analytical specificity. Finally, our assays were combined with already published diagnostic methods to a diagnostic workflow that enables time-saving, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.


2015 ◽  
Vol 81 ◽  
pp. 38-46 ◽  
Author(s):  
J.P.R. Sorensen ◽  
D.J. Lapworth ◽  
B.P. Marchant ◽  
D.C.W. Nkhuwa ◽  
S. Pedley ◽  
...  

2015 ◽  
Vol 88 (1) ◽  
pp. 115-119 ◽  
Author(s):  
William A. Glover ◽  
Ederlyn E. Atienza ◽  
Shannon Nesbitt ◽  
Woo J. Kim ◽  
Jared Castor ◽  
...  
Keyword(s):  
Bk Virus ◽  

2009 ◽  
Vol 43 (11) ◽  
pp. 2829-2840 ◽  
Author(s):  
Andrew R. Dzialowski ◽  
Val H. Smith ◽  
Donald G. Huggins ◽  
Frank deNoyelles ◽  
Niang-Choo Lim ◽  
...  

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