Rational Engineering of Cofactor Specificity of Glutamate Dehydrogenase for Poly-γ-Glutamic Acid Synthesis in Bacillus licheniformis

2021 ◽  
pp. 109979
Author(s):  
Fan Yang ◽  
Na Liu ◽  
Yaozhong Chen ◽  
Si Wang ◽  
Jun Liu ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamate Dehydrogenase ◽  
Bacillus Licheniformis ◽  
Acid Synthesis ◽  
Cofactor Specificity ◽  
Rational Engineering

Calcium regulates glutamate dehydrogenase and poly-γ-glutamic acid synthesis in Bacillus natto

2015 ◽  
Vol 38 (4) ◽  
pp. 673-679 ◽  
Author(s):  
Yonghong Meng ◽  
Guiru Dong ◽  
Chen Zhang ◽  
Yuanyuan Ren ◽  
Yuling Qu ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamate Dehydrogenase ◽  
Acid Synthesis ◽  
Bacillus Natto

Physiological and metabolic analysis of nitrate reduction on poly-gamma-glutamic acid synthesis in Bacillus licheniformis WX-02

2014 ◽  
Vol 196 (11) ◽  
pp. 791-799 ◽  
Author(s):  
Xin Li ◽  
Xiangyong Gou ◽  
Dan Long ◽  
Zhixia Ji ◽  
Lifang Hu ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Bacillus Licheniformis ◽  
Nitrate Reduction ◽  
Acid Synthesis ◽  
Metabolic Analysis

scholarly journals Promoting Cell Growth And Poly-Y-Glutamic Acid Production By Boosting The Synthesis of Alanine And D-Alanyl-D-Alanine In Bacillus Licheniformis

2022 ◽  
Author(s):  
Zheng Zhang ◽  
Penghui He ◽  
Shiying Hu ◽  
Yanqing Yu ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Microbial Biomass ◽  
Glutamic Acid ◽  
Bacillus Licheniformis ◽  
Alanine Racemase ◽  
High Yield ◽  
Effective Strategy ◽  
Genes Encoding ◽  
Alanine Synthesis ◽  
Glutamic Acid Production

Abstract Objective: The production of some bio-chemicals affected by the cell growth. This study aimed at promoting the cell growth by overexpressing the synthesis of peptidoglycans tetrapeptide tail components to improve poly-γ-glutamic acid (γ-PGA) production. Results: L-alanine, D-alanine and D-alanyl-D-alanine are primary precursors for the synthesis of peptidoglycans. The addition of L-alanine and D-alanine significantly increased both the cell growth and production of γ-PGA. Then, several genes encoding key enzymes for L/D-alanine and D-alanyl-D-alanine biosynthesis were overexpressed respectively, including ald (encoding alanine dehydrogenase), dal (encoding alanine racemase) and ddl (encoding D-alanine ligase). The results showed that the overexpression of genes ald , dal and ddl increased the production of γ-PGA by 19.72%, 15.91% and 60.90%, and increased the microbial biomass by 15.58%, 18.34% and 49.85%, respectively. Moreover, we demonstrated that the overexpression of genes ald , dal and ddl increased γ-PGA production mainly by enhancing cell growth rather than providing more precursors. Conclusions: This work illustrated the importance of the L/D-alanine and D-alanyl-D-alanine synthesis to the cell growth and the high yield of γ-PGA, and provided an effective strategy for producing γ-PGA .

N,N-Dimethylsphingosine conjugates of poly-l-glutamic acid: Synthesis, characterization, and initial biological evaluation

2009 ◽  
Vol 19 (3) ◽  
pp. 1012-1017 ◽  
Author(s):  
Sukhen C. Ghosh ◽  
Edmond Auzenne ◽  
Mojgan Khodadadian ◽  
David Farquhar ◽  
Jim Klostergaard
Keyword(s):  
Glutamic Acid ◽  
Acid Synthesis ◽  
Biological Evaluation
Sign in / Sign up

Export Citation Format

Share Document