Ultrastructural changes in the tegument and gut of adult Fasciola hepatica following in vivo treatment with artesunate

2015 ◽  
Vol 154 ◽  
pp. 143-154 ◽  
Author(s):  
J.F. O'Neill ◽  
R.C. Johnston ◽  
L. Halferty ◽  
G.P. Brennan ◽  
I. Fairweather
Keyword(s):  
Fasciola Hepatica ◽  
Ultrastructural Changes ◽  
In Vivo Treatment

Ultrastructural changes to the tegumental system and gastrodermal cells of adult Fasciola hepatica following treatment in vivo with a commercial preparation of myrrh (Mirazid)

2016 ◽  
Vol 91 (6) ◽  
pp. 672-685 ◽  
Author(s):  
M.M.O. Abdelaal ◽  
G.P. Brennan ◽  
A. Abdel-Aziz ◽  
I. Fairweather
Keyword(s):  
Fasciola Hepatica ◽  
Ultrastructural Changes ◽  
Muscle Fibres ◽  
Somatic Muscle ◽  
Secretory Body ◽  
Commercial Preparation ◽  
Transmission Electron ◽  
Body Production ◽  
Tegumental Syncytium

AbstractAn in vivo study in the laboratory rat model has been carried out to monitor changes to the tegument and gut of adult Fasciola hepatica following treatment with myrrh (‘Mirazid’). Rats infected with the triclabendazole-resistant Dutch isolate were dosed orally with Mirazid at a concentration of 250 mg/kg and flukes recovered 2, 3 and 7 days post-treatment (pt). The flukes were processed for examination by scanning and transmission electron microscopy. A variety of changes to the external surface were observed, culminating in the sloughing of the tegumental syncytium. Internal changes to the syncytium and tegumental cell bodies were more severe and were evident from 2 days pt onwards. Swelling of the basal infolds (leading to flooding of the surface layer) and a decline in secretory body production were the major changes seen. The gastrodermal cells were less severely affected than the tegument, pointing to a trans-tegumental route of uptake for Mirazid by the fluke. Some loss of muscle fibres in the main somatic muscle layers was observed, which may be correlated with the decline in movement of flukes seen at recovery.

Ultrastructural changes induced in the tegument and gut of Fasciola hepatica following in vivo and in vitro drug treatment with nitroxynil (Trodax)

2007 ◽  
Vol 101 (4) ◽  
pp. 929-941 ◽  
Author(s):  
B. McKinstry ◽  
G. P. Brennan ◽  
L. Halferty ◽  
A. B. Forbes ◽  
I. Fairweather
Keyword(s):  
Drug Treatment ◽  
Fasciola Hepatica ◽  
Ultrastructural Changes

Fasciola hepatica: time-dependent disruption of spermatogenesis following in vivo treatment with triclabendazole

2011 ◽  
Vol 109 (4) ◽  
pp. 1035-1043 ◽  
Author(s):  
Emma Toner ◽  
Gerard P. Brennan ◽  
Robert E. B. Hanna ◽  
Hillary W. J. Edgar ◽  
Ian Fairweather
Keyword(s):  
Fasciola Hepatica ◽  
Time Dependent ◽  
In Vivo Treatment

Early onset of changes to the reproductive system of Fasciola hepatica following in vivo treatment with triclabendazole

2012 ◽  
Vol 184 (2-4) ◽  
pp. 341-347 ◽  
Author(s):  
R.E.B. Hanna ◽  
S. Scarcella ◽  
H. Solana ◽  
S. McConnell ◽  
I. Fairweather
Keyword(s):  
Reproductive System ◽  
Early Onset ◽  
Fasciola Hepatica ◽  
In Vivo Treatment

Ultrastructural changes to the tegumental system and the gastrodermal cells in adult Fasciola hepatica following in vivo treatment with the experimental fasciolicide, compound alpha

Parasitology ◽  
2009 ◽  
Vol 136 (6) ◽  
pp. 665-680 ◽  
Author(s):  
M. McCONVILLE ◽  
G. P. BRENNAN ◽  
A. FLANAGAN ◽  
H. W. J. EDGAR ◽  
R. CASTILLO ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Fasciola Hepatica ◽  
Secretory Activity ◽  
Ultrastructural Changes ◽  
Muscle Fibres ◽  
Somatic Muscle ◽  
Tegumental Cell ◽  
Transmission Electron ◽  
Tegumental Syncytium

SUMMARYSheep infected with the triclabendazole-susceptible, Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48 and 72 h post-treatment (p.t.). Ultrastructural changes to the flukes were assessed using transmission electron microscopy (TEM), with a view to gathering information on the mechanism(s) of action for compound alpha and on the possible route of its entry into F. hepatica. The tegumental syncytium was more severely affected than the gut at all time-points p.t. with compound alpha, suggesting a predominantly trans-tegumental route of uptake. Disruption to the tegumental system became increasingly severe over time. A stress response was observed at 24 h p.t. and took the form of blebbing and increases in the production and transport of secretory bodies. By 72 h p.t., extensive tegumental loss and degeneration of the tegumental cell bodies had occurred. Degeneration of subtegumental tissues and internal flooding were also observed. Changes in the gastrodermal cells were slow to develop: reduced secretory activity was evident at 72 h p.t.. There was progressive disruption to the somatic muscle layers, with disorganization of the muscle blocks and loss of muscle fibres.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Morphometric Analysis of the Hepatocytes from Fish Exposed to Arsenic

1976 ◽  
Vol 34 ◽  
pp. 282-283
Author(s):  
Elsie M. B. Sorensen
Keyword(s):  
Morphometric Analysis ◽  
Arsenic Concentration ◽  
Ultrastructural Changes ◽  
Sodium Arsenate ◽  
Lepomis Cyanellus ◽  
Mammalian Counterpart ◽  
Intracellular Changes ◽  
Green Sunfish ◽  
Organic Pesticides

The detoxification capacity of the liver is well documented for a variety of substances including ethanol, organic pesticides, drugs, and metals. The piscean liver, although less enzymatically active than the mammalian counterpart (1), contains endoplasmic reticulum with an impressive repertoire of oxidizing, reducing, and conjugating abilities (2). Histopathologic changes are kncwn to occur in fish hepatocytes following in vivo exposure to arsenic (3); however, ultrastructural changes have not been reported. This study involved the morphometric analysis of intracellular changes in fish parynchymal hepatocytes and correlation with arsenic concentration in the liver.Green sunfish (Lepomis cyanellus, R.) were exposed to 0, 30, or 60 ppm arsenic (as sodium arsenate) at 20°C for 1, 2, or 3 week intervals before removal of livers for quantification of the arsenic burden (using neutron activation analysis) and morphometric analysis of ultrastructural alterations. Livers were cut into 1 mm cubes for fixation, dehydration, and embedding.

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