Immature Fasciola gigantica: Time-dependent ultrastructural changes following in vivo treatment with triclabendazole

(1) Background: The study aimed to assess neurobehavioral, ultrastructural, and biochemical changes induced by silver nanoparticles synthesized with Cornus mas L. extract (AgNPs-CM) in rat brains. (2) Methods: The study included 36 male adult rats divided into three groups. Over a period of 45 days, AgNPs-CM (0.8 and 1.5 mg/kg b.w.) were administered daily by gavage to two of the groups, while the control group received the vehicle used for AgNP. After treatment, OFT and EPM tests were conducted in order to assess neurobehavioral changes. Six of the animals from each group were sacrificed immediately after completion of treatment, while the remaining six were allowed to recuperate for an additional 15 days. Transmission electron microscopy (TEM), GFAP immunohistochemistry, and evaluation of TNFα, IL-6, MDA, and CAT activity were performed on the frontal cortex and hippocampus. (3) Results: Treated animals displayed a dose- and time-dependent increase in anxiety-like behavior and severe ultrastructural changes in neurons, astrocytes, and capillaries in both brain regions. Immunohistochemistry displayed astrogliosis with altered cell morphology. TNFα, IL-6, MDA, and CAT activity were significantly altered, depending on brain region and time post exposure. (4) Conclusions: AgNPs-CM induced neurobehavioral changes and severe cell lesions that continued to escalate after cessation of exposure.

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Fasciola hepatica: time-dependent disruption of spermatogenesis following in vivo treatment with triclabendazole

Parasitology Research ◽

10.1007/s00436-011-2341-4 ◽

2011 ◽

Vol 109 (4) ◽

pp. 1035-1043 ◽

Cited By ~ 14

Author(s):

Emma Toner ◽

Gerard P. Brennan ◽

Robert E. B. Hanna ◽

Hillary W. J. Edgar ◽

Ian Fairweather

Keyword(s):

Fasciola Hepatica ◽

Time Dependent ◽

In Vivo Treatment

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T.P.2.13 Evaluation of time-dependent effects of statins and fibrate ‘in vivo’ treatment on ClC-1 chloride channel expression and function and on proteomic profile of rat skeletal muscle

Neuromuscular Disorders ◽

10.1016/j.nmd.2009.06.129 ◽

2009 ◽

Vol 19 (8-9) ◽

pp. 583-584

Author(s):

G.M. Camerino ◽

S. Pierno ◽

C. Digennaro ◽

M.M. Dinardo ◽

M.A. Pellegrino ◽

...

Keyword(s):

Skeletal Muscle ◽

Chloride Channel ◽

Time Dependent ◽

Proteomic Profile ◽

Rat Skeletal Muscle ◽

Channel Expression ◽

In Vivo Treatment ◽

And Function

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Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050287 ◽

1974 ◽

Vol 32 ◽

pp. 54-55

Author(s):

S. Phyllis Steamer ◽

Rosemarie L. Devine

Keyword(s):

Structural Changes ◽

Radiation Treatment ◽

Arterial Stenosis ◽

Ultrastructural Changes ◽

Vascular Effects ◽

Microvascular Changes ◽

Surrounding Connective Tissue ◽

Fission Neutrons ◽

Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

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Morphometric Analysis of the Hepatocytes from Fish Exposed to Arsenic

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091391 ◽

1976 ◽

Vol 34 ◽

pp. 282-283

Author(s):

Elsie M. B. Sorensen

Keyword(s):

Morphometric Analysis ◽

Arsenic Concentration ◽

Ultrastructural Changes ◽

Sodium Arsenate ◽

Lepomis Cyanellus ◽

Mammalian Counterpart ◽

Intracellular Changes ◽

Green Sunfish ◽

Organic Pesticides

The detoxification capacity of the liver is well documented for a variety of substances including ethanol, organic pesticides, drugs, and metals. The piscean liver, although less enzymatically active than the mammalian counterpart (1), contains endoplasmic reticulum with an impressive repertoire of oxidizing, reducing, and conjugating abilities (2). Histopathologic changes are kncwn to occur in fish hepatocytes following in vivo exposure to arsenic (3); however, ultrastructural changes have not been reported. This study involved the morphometric analysis of intracellular changes in fish parynchymal hepatocytes and correlation with arsenic concentration in the liver.Green sunfish (Lepomis cyanellus, R.) were exposed to 0, 30, or 60 ppm arsenic (as sodium arsenate) at 20°C for 1, 2, or 3 week intervals before removal of livers for quantification of the arsenic burden (using neutron activation analysis) and morphometric analysis of ultrastructural alterations. Livers were cut into 1 mm cubes for fixation, dehydration, and embedding.

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Changes in Granulocyte Fibrinolytic Activity (FA) Induced by In Vivo Treatment with Lysine Acetylsalicylate (LAS)

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653473 ◽

1981 ◽

Vol 46 (04) ◽

pp. 761-761 ◽

Cited By ~ 2

Author(s):

Franco Ghezzo ◽

Paola Trinchero ◽

Luigi Pegoraro

Keyword(s):

Fibrinolytic Activity ◽

Lysine Acetylsalicylate ◽

In Vivo Treatment

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The Natural Flavonoid Naringenin Inhibits the Cell Growth of WilmsTumorinChildren by Suppressing TLR4/NF-κB Signaling

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620999200818155814 ◽

2020 ◽

Vol 20 ◽

Author(s):

Hongtao Li ◽

Peng Chen ◽

Lei Chen ◽

Xinning Wang

Keyword(s):

Cell Growth ◽

Western Blot ◽

Wilms Tumor ◽

Critical Role ◽

Time Dependent ◽

Luciferase Assay ◽

Dependent Manner ◽

Tlr4 Expression ◽

Protein Levels

Background: Nuclear factor kappa B (NF-κB) is usually activated in Wilms tumor (WT) cells and plays a critical role in WT development. Objective: The study purpose was to screen a NF-κB inhibitor from natural product library and explore its effects on WT development. Methods: Luciferase assay was employed to assess the effects of natural chemical son NF-κB activity. CCK-8 assay was conducted to assess cell growth in response to naringenin. WT xenograft model was established to analyze the effect of naringenin in vivo. Quantitative real-time PCR and Western blot were performed to examine the mRNA and protein levels of relative genes, respectively. Results: Naringenin displayed significant inhibitory effect on NF-κB activation in SK-NEP-1 cells. In SK-NEP-1 and G-401 cells, naringenin inhibited p65 phosphorylation. Moreover, naringenin suppressed TNF-α-induced p65 phosphorylation in WT cells. Naringenin inhibited TLR4 expression at both mRNA and protein levels in WT cells. CCK-8 staining showed that naringenin inhibited cell growth of the two above WT cells in dose-and time-dependent manner, whereas Toll-like receptor 4 (TLR4) over expression partially reversed the above phenomena. Besides, naringenin suppressed WT tumor growth in dose-and time-dependent manner in vivo. Western blot found that naringenin inhibited TLR4 expression and p65 phosphorylation in WT xenograft tumors. Conclusion: Naringenin inhibits WT development viasuppressing TLR4/NF-κB signaling

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