Cholinium chloride as a weak salting-out agent to tune the biomolecules partition behavior in polymer-salt aqueous two-phase systems

2020 ◽  
Vol 124 ◽  
pp. 48-56
Author(s):  
José Pedro Wojeicchowski ◽  
Fabiane Oliveira Farias ◽  
Rodrigo Thibes Gonsalves ◽  
Carlos Itsuo Yamamoto ◽  
Luciana Igarashi-Mafra ◽  
...  
Keyword(s):  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partition Behavior

Research the Equilibrium Distribution of Puerarin in Aqueous Two-Phase Combine with Temperature-Induced Phase Separation EOPO/ K2HPO4 /Water Systems

2011 ◽  
Vol 138-139 ◽  
pp. 1162-1167
Author(s):  
Xue Mei Gu ◽  
Qing Huo ◽  
Qiang Lin
Keyword(s):  
Partition Coefficients ◽  
Equilibrium Distribution ◽  
Random Copolymer ◽  
Water Systems ◽  
Optimum Conditions ◽  
Two Phase ◽  
Modified Model ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partition Behavior

The partition behavior of Puerarin in ethylene oxide-propylene oxide random copolymer (EOPO)/ K2HPO4/water systems was investigated .The optimum conditions were EOPO （SDP30） 28%, K2HPO424%, temperature-induced condition was 70°C and 30min. yield of Puerarin could reach 42.26%. Based on the modified model lnK/R2=A*+b*(w”-w’)+c*(w”-w’)2, partition coefficients of Puerarin in EOPO/K2HPO4aqueous two-phase systems were correlated. It was found that the correlation of the experimental data was quite satisfactory.

scholarly journals Liquid-liquid equilibrium of novel aqueous two-phase systems and evaluation of salting-out abilities of salts

2010 ◽  
Vol 8 (4) ◽  
pp. 886-891 ◽  
Author(s):  
Yun Wang ◽  
Jing Wang ◽  
Juan Han ◽  
Shiping Hu ◽  
Yongsheng Yan
Keyword(s):  
Water Systems ◽  
Organic Salt ◽  
Excluded Volume ◽  
Statistical Geometry ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Aqueous Two Phase Extraction

AbstractBinodal data are beneficial to the design of aqueous two-phase extraction and the establishment of thermodynamic models. For the 2-propanol + Li2SO4/Na2SO4/ (NH4)2SO4/K3PO4 + water systems and 1-propanol + K3PO4/K3C6H5O7/(NH4)3C6H5O7 + water systems, binodal data were determined at 298.15 K. The binodal data were correlated by a theoretical equation on the basis of statistical geometry. The salting-out abilities of salts and the phase-separation abilities of alcohols were evaluated by the effective excluded volume of salt and the binodal curves plotted in molality. A simple Hofmeister series of cations and anions were obtained. The organic salt, K3C6H5O7, shows as high a salting-out ability as K3PO4.

scholarly journals Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

2019 ◽  
Vol 141 ◽  
pp. 239-246 ◽  
Author(s):  
Catarina M.S.S. Neves ◽  
Rita de Cássia S. Sousa ◽  
Matheus M. Pereira ◽  
Mara G. Freire ◽  
João A.P. Coutinho
Keyword(s):  
Ionic Liquids ◽  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Liquid-Liquid Equilibrium Data for the Ionic Liquid N-Ethyl-Pyridinium Bromide with Several Sodium Salts and Potassium Salts

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Yuliang Li ◽  
Xiaojia Lu ◽  
Jing Hao ◽  
Changquan Chen
Keyword(s):  
Correlation Coefficients ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Salting Out ◽  
Pyridinium Bromide ◽  
Equilibrium Data ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Lle Data ◽  
Tie Line

The liquid-liquid equilibrium (LLE) data for systems containing N-ethyl-pyridinium bromide ([EPy]Br), salt (Na2HPO4,K2HPO4,K2SO4,C4O6H4KNa), and water have been measured experimentally atT=298.15 K and the formations of these four aqueous two-phase systems (ATPSs) have been discussed. Also, the effective excluded volume (EEV) values obtained from the binodal models for the four systems were determined and the salting-out abilities of different salts follow the order ofK2SO4>K2HPO4>Na2HPO4>C4O6H4KNa. The solubility data were correlated by the Merchuk and other equations while the tie-line data by the Othmer-Tobias, Bancroft, two-parameter, and Setschenow-type equations. The correlation coefficients evidenced that experimental data fitted well to all these equations. These four salts were proved successfully to form ATPSs with N-ethyl-pyridinium bromide, making a significant contribution to the further study of this kind of ATPS.

How to manipulate partition behavior of proteins in aqueous two-phase systems: Effect of trimethylamine N-oxide (TMAO)

2017 ◽  
Vol 449 ◽  
pp. 217-224 ◽  
Author(s):  
Luisa A. Ferreira ◽  
Zhonghua Wu ◽  
Lukasz Kurgan ◽  
Vladimir N. Uversky ◽  
Boris Y. Zaslavsky
Keyword(s):  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partition Behavior

Evaluation of salting-out effect in the liquid–liquid equilibrium of aqueous two-phase systems composed of 2-propanol and Na2SO4/MgSO4 at different temperatures

2017 ◽  
Vol 450 ◽  
pp. 184-193 ◽  
Author(s):  
Juliana Gomes Pimentel ◽  
Silvana Ferreira Bicalho ◽  
Olga Reinert Ramos Gandolfi ◽  
Lizzy Ayra Alcântara Verissimo ◽  
Sérgio de Sousa Castro ◽  
...  
Keyword(s):  
Liquid Equilibrium ◽  
Two Phase ◽  
Salting Out ◽  
Different Temperatures ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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