Timing of sperm capacitation appears to be programmed according to egg availability in the female genital tract

2004 ◽  
Vol 82 (1) ◽  
pp. 247-249 ◽  
Author(s):  
Laura C Giojalas ◽  
Roberto A Rovasio ◽  
Georgina Fabro ◽  
Anna Gakamsky ◽  
Michael Eisenbach
Keyword(s):  
Genital Tract ◽  
Female Genital Tract ◽  
Sperm Capacitation ◽  
Female Genital

scholarly journals Rabbit semen fertility after heterospermic insemination with spermatozoa of different capacitation time

2018 ◽  
Vol 49 (2) ◽  
pp. 143
Author(s):  
P. YPSILANTIS (Π. ΥΨΗΛΑΝΤΗΣ) ◽  
Ph. SARATSIS (Φ. ΣΑΡΑΤΣΗΣ) ◽  
S. SAMOUILIDIS (Σ. ΣΑΜΟΥΗΛΙΔΗΣ)
Keyword(s):  
Litter Size ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Group Differences ◽  
Sperm Capacitation ◽  
Group 4 ◽  
Female Genital

The influence of mixing heterogeneous sperm populations in rabbit semen fertility was studied. Eighty female rabbits which were divided in 4 groups (1,2,3 and 4) in = 20.) and 3 males of proven fertility were employed. The animals of group 1,2 and 3 were inseminated with semen from male A j, A2 and A3, respectively, while those of group 4 were inseminated with a mixture containing equal number of progressively motile spermatozoa from each of the above mentioned males ( A 1 + 2 + 3 ) (heterospermic insemination). Animals of each group were divided into 4 subgroups according to the insemination time (15,10, 5 and 0 hours prior the expected ovulation). In each group, differences were observed (P<0.05) between subgroups at the percentage of animals that delivered, indicating differences between males at the time and the duration of sperm capacitation in vivo. Based on the percentage of the animals that delivered and the litter size, semen fertility was improved after the application of heterospermic insemination, at all insemination times. This improvement was attributed to the extension of the time during which capacitated spermatozoa were present in the female genital tract due to the mixture of heterogeneous sperm populations of different capacitation time.

scholarly journals Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 648 ◽  
Author(s):  
Diana N. Raju ◽  
Jan N. Hansen ◽  
Sebastian Rassmann ◽  
Birthe Stüven ◽  
Jan F. Jikeli ◽  
...  
Keyword(s):  
Genital Tract ◽  
Physiological Function ◽  
Female Genital Tract ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Sperm Capacitation ◽  
Pharmacological Interventions ◽  
Sperm Flagellum ◽  
Mammalian Sperm ◽  
Female Genital

Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3′,5′-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To this end, we used sperm that express the light-activated phosphodiesterase LAPD or the photo-activated adenylate cyclase bPAC. The control of cAMP by LAPD or bPAC combined with pharmacological interventions provides spatiotemporal precision and allows to probe the physiological function of cAMP compartmentalization in mammalian sperm.

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