scholarly journals SUPRAPHYSIOLOGIC SERUM ESTRADIOL NEGATIVELY IMPACTS EUPLOID EMBRYO IMPLANTATION

2021 ◽  
Vol 116 (3) ◽  
pp. e310
Author(s):  
Katherine L. Palmerola ◽  
Sabrina A. Gerkowicz ◽  
Antonella Lozano ◽  
Marta Montenegro ◽  
Ineabelle Collazo ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Serum Estradiol ◽  
Euploid Embryo

scholarly journals Is day 5 blastocyst better than day 6 blastocyst? Evidence from NGS-based PGT-A results

2021 ◽  
Author(s):  
Ting Zhang
Keyword(s):  
Maternal Age ◽  
Embryo Implantation ◽  
Trophectoderm Biopsy ◽  
Preimplantation Genetic Testing ◽  
Non Invasive ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Ploidy Status ◽  
Better Than ◽  
Euploid Embryo

Abstract Background Aneuploidy is the principal genetic factor leading to the failure of embryo implantation. For most patients who accept the non-preimplantation genetic testing (PGT) cycle, non-invasive methods to select euploid embryos with the best pregnancy potential are desirable Methods This retrospective study recruited women undergoing PGT for aneuploidy (PGT-A) with trophectoderm biopsy from January 2019 to December 2020. The ploidy status of embryos was determined by next generation sequencing (NGS). Results Altogether 2531 blastocysts from 839 PGT-A cycles were evaluated. The euploid rate of day 5 blastocysts seemed to be significantly higher than that of day 6 blastocysts, either from the same ovarian stimulation (OS) cycles (49.9% vs 35.7%, P < 0.001) or from different OS cycles (48.2% vs 27.8%, P < 0.001). Both the younger maternal age (adjusted OR = 0.917, 95% CI: 0.892–0.944, P < 0.001) and day 5 stage (adjusted OR = 1.735, 95% CI: 1.415–2.127, P < 0.001) were independently associated with the greater euploid rate of blastocysts. However, after single euploid embryo transfer, the clinical outcomes of day 5 blastocysts were comparable to those of day 6 blastocysts, no matter whether they were from the same OS cycles or not. Conclusions Our results revealed that day 5 blastocysts possess a higher euploid rate than day 6 blastocysts independent of the OS cycles. Giving priority to a day 5 blastocyst over day 6 blastocyst will increase the likelihood to select single euploid embryo for transfer in non-PGT cycles.

Evidence for an adverse effect of elevated serum estradiol concentrations on embryo implantation

1988 ◽  
Vol 49 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Robert Forman ◽  
Joelle Belaisch-Allart ◽  
Nicholas Fries ◽  
Andre Hazout ◽  
Jacques Testart ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Embryo Implantation ◽  
Elevated Serum ◽  
Serum Estradiol

scholarly journals Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium

2017 ◽  
Vol 59 (2) ◽  
pp. 105-119 ◽  
Author(s):  
Kamran Ullah ◽  
Tanzil Ur Rahman ◽  
Hai-Tao Pan ◽  
Meng-Xi Guo ◽  
Xin-Yan Dong ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Controlled Ovarian Hyperstimulation ◽  
Endometrial Receptivity ◽  
Protein Profiling ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Serum Estradiol ◽  
Endometrial Cells ◽  
Ishikawa Cells ◽  
Estradiol Levels

Previous studies have shown that increasing estradiol concentrations had a toxic effect on the embryo and were deleterious to embryo adhesion. In this study, we evaluated the physiological impact of estradiol concentrations on endometrial cells to reveal that serum estradiol levels probably targeted the endometrium in controlled ovarian hyperstimulation (COH) protocols. An attachment model of human choriocarcinoma (JAr) cell spheroids to receptive-phase endometrial epithelial cells and Ishikawa cells treated with different estradiol (10−9 M or 10−7 M) concentrations was developed. Differentially expressed protein profiling of the Ishikawa cells was performed by proteomic analysis. Estradiol at 10−7 M demonstrated a high attachment rate of JAr spheroids to the endometrial cell monolayers. Using iTRAQ coupled with LC–MS/MS, we identified 45 differentially expressed proteins containing 43 significantly upregulated and 2 downregulated proteins in Ishikawa cells treated with 10−7 M estradiol. Differential expression of C3, plasminogen and kininogen-1 by Western blot confirmed the proteomic results. C3, plasminogen and kininogen-1 localization in human receptive endometrial luminal epithelium highlighted the key proteins as possible targets for endometrial receptivity and interception. Ingenuity pathway analysis of differentially expressed proteins exhibited a variety of signaling pathways, including LXR/RXR activation pathway and acute-phase response signaling and upstream regulators (TNF, IL6, Hmgn3 and miR-140-3p) associated with endometrial receptivity. The observed estrogenic effect on differential proteome dynamics in Ishikawa cells indicates that the human endometrium is the probable target for serum estradiol levels in COH cycles. The findings are also important for future functional studies with the identified proteins that may influence embryo implantation.

scholarly journals Chronic endometritis screening in patients who experience euploid embryo implantation failure does not improve IVF outcomes after a subsequent euploid FET

2019 ◽  
Vol 112 (3) ◽  
pp. e166
Author(s):  
Carlos Hernandez-Nieto ◽  
Joseph A. Lee ◽  
Daniel E. Stein ◽  
Tanmoy Mukherjee ◽  
Benjamin Sandler ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Implantation Failure ◽  
Chronic Endometritis ◽  
Ivf Outcomes ◽  
Euploid Embryo

scholarly journals Description of euploid embryo implantation outcome by morphokinetic investigation

2015 ◽  
Vol 104 (3) ◽  
pp. e283-e284
Author(s):  
A. Tejera ◽  
M. Stoppa ◽  
M. Meseguer ◽  
A. Capalbo ◽  
M. Florensa ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Euploid Embryo

CHRONIC ENDOMETRITIS (CE) BY CD138 IN AN INFERTILE POPULATION: A NON-SELECTION STUDY DISCOVERING BASELINE PREVALENCE AND EFFECT ON EARLY EUPLOID EMBRYO IMPLANTATION

2020 ◽  
Vol 114 (3) ◽  
pp. e210
Author(s):  
Jennifer K. Blakemore ◽  
David L. Keefe ◽  
David H. McCulloh ◽  
Alexis Masbou ◽  
James A. Grifo
Keyword(s):  
Embryo Implantation ◽  
Chronic Endometritis ◽  
Euploid Embryo

scholarly journals THE SECRETOME OF THE EUPLOID EMBRYO IMPLANTATION

2021 ◽  
Vol 116 (3) ◽  
pp. e146-e147
Author(s):  
Mariana Moraes Piccolomini ◽  
Lucas Yamakami ◽  
Irineu s Massaia ◽  
Renato Tomioka ◽  
Edson G. Lo Turco ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Euploid Embryo

Efek Kadar Serum Estradiol Fase Bleeding Siklus Menstruasi Pada Aktivitas Fibroblas Dermis Manusia

2019 ◽  
Vol 45 (1) ◽  
Author(s):  
Graciella Regina ◽  
Sri Awalia Febriana ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Mtt Assay ◽  
Ex Vivo ◽  
Serum Estradiol ◽  
Sirius Red

Pembedahan elektif selama fase bleeding siklus menstruasi sering dihindari terkait gangguan koagulasi. Salah satu hormon yang berhubungan dengan proses penyembuhan luka adalah estrogen. Kadar estrogen berfluktuasi sepanjang siklus menstruasi dan berada pada kadar terendah selama fase bleeding. Penelitian eksperimental ex vivo dilakukan pada 16 perempuan berusia 18–40 tahun yang memiliki siklus menstruasi teratur. Darah vena subjek diambil sebanyak 5ml pada fase bleeding dan ovulasi. Kemampuan penyembuhan luka dari masing-masing serum dinilai dengan mengukur proliferasi fibroblas dan deposisi kolagen fibroblas kulit. Ovulasi ditentukan dengan uji pakis saliva, kadar estradiol serum diukur menggunakan Cobas Elecsys®, proliferasi fibroblas menggunakan MTT assay, dan deposisi kolagen dengan sirius red. Hasil penelitian menunjukkan rerata kadar serum estradiol pada fase bleeding dan ovulasi berturut-turut adalah 29,6±10,5pg/dl dan 180,1±164,5pg/dl. Rerata indeks proliferasi fibroblas yang dipajankan pada fase bleeding dan ovulasi adalah 1,09±0,63 dan 1,44±0,66. Rerata densitas optik kolagen fibroblas yang terpajan serum fase bleeding dan ovulasi adalah 0,47±0,2 dan 0,54±0,14. Seluruhnya menunjukkan perbedaan yang bermakna secara statistik (p<0,05). Serum fase bleeding memiliki kemampuan penyembuhan luka yang lebih rendah dibandingkan dengan serum fase ovulasi. Kebijakan untuk tidak melakukan pembedahan elektif selama fase bleeding, selain terkait dengan gangguan pembekuan darah juga terkait dengan proses penyembuhan luka.Kata Kunci:  menstruasi, estradiol, ovulasi, proliferasi fibroblas, deposisi kolagen, penyembuhan luka

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