The MpkA MAP kinase module regulates cell wall integrity signaling and pyomelanin formation in Aspergillus fumigatus

2009 ◽  
Vol 46 (12) ◽  
pp. 909-918 ◽  
Author(s):  
Vito Valiante ◽  
Radhika Jain ◽  
Thorsten Heinekamp ◽  
Axel A. Brakhage
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Map Kinase ◽  
Cell Wall Integrity

AfMkk2 is required for cell wall integrity signaling, adhesion, and full virulence of the human pathogen Aspergillus fumigatus

2010 ◽  
Vol 300 (7) ◽  
pp. 496-502 ◽  
Author(s):  
Franziska Dirr ◽  
Bernd Echtenacher ◽  
Jürgen Heesemann ◽  
Petra Hoffmann ◽  
Frank Ebel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Cell Wall Integrity ◽  
Human Pathogen

Pim1, a MAP kinase involved in cell wall integrity in Pichia pastoris

2001 ◽  
Vol 265 (4) ◽  
pp. 604-614 ◽  
Author(s):  
I.C. Cosano ◽  
H. Martín ◽  
M. Flández ◽  
C. Nombela ◽  
M. Molina
Keyword(s):  
Cell Wall ◽  
Pichia Pastoris ◽  
Map Kinase ◽  
Cell Wall Integrity

scholarly journals Phosphorylation of Aspergillus fumigatus PkaR impacts growth and cell wall integrity through novel mechanisms

FEBS Letters ◽  
2017 ◽  
Vol 591 (22) ◽  
pp. 3730-3744 ◽  
Author(s):  
Elliot K. Shwab ◽  
Praveen R. Juvvadi ◽  
Greg Waitt ◽  
Erik J. Soderblom ◽  
Martin A. Moseley ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Cell Wall Integrity

scholarly journals Geranylgeranyltransferase Cwg2-Rho4/Rho5 module is implicated in the Pmk1 MAP kinase-mediated cell wall integrity pathway in fission yeast

2015 ◽  
Vol 20 (4) ◽  
pp. 310-323 ◽  
Author(s):  
Akira Doi ◽  
Ayako Kita ◽  
Yuki Kanda ◽  
Takaya Uno ◽  
Keita Asami ◽  
...  
Keyword(s):  
Cell Wall ◽  
Map Kinase ◽  
Fission Yeast ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase C

PLoS Genetics ◽  
2014 ◽  
Vol 10 (9) ◽  
pp. e1004582 ◽  
Author(s):  
Ines Teichert ◽  
Eva Katharina Steffens ◽  
Nicole Schnaß ◽  
Benjamin Fränzel ◽  
Christoph Krisp ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Map Kinase ◽  
Scaffold Protein ◽  
Cell Wall Integrity ◽  
Activator Protein ◽  
Kinase C ◽  
Cell Wall Integrity Pathway

scholarly journals O-Mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) Is Crucial for Cell Wall Integrity and Conidium Morphology, Especially at an Elevated Temperature

2007 ◽  
Vol 6 (12) ◽  
pp. 2260-2268 ◽  
Author(s):  
Hui Zhou ◽  
Hongyan Hu ◽  
Lijuan Zhang ◽  
Ruoyu Li ◽  
Haomiao Ouyang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Elevated Temperature ◽  
Aspergillus Fumigatus ◽  
Cell Wall Integrity ◽  
Secretory Proteins ◽  
Experimental Conditions ◽  
Human Fungal Pathogen ◽  
Conidium Formation

ABSTRACT Protein O-mannosyltransferases initiate O mannosylation of secretory proteins, which are of fundamental importance in eukaryotes. In this study, the PMT gene family of the human fungal pathogen Aspergillus fumigatus was identified and characterized. Unlike the case in Saccharomyces cerevisiae, where the PMT family is highly redundant, only one member of each PMT subfamily, namely, Afpmt1, Afpmt2, and Afpmt4, is present in A. fumigatus. Mutants with a deletion of Afpmt1 are viable. In vitro and in vivo activity assays confirmed that the protein encoded by Afpmt1 acts as an O-mannosyltransferase (AfPmt1p). Characterization of the ΔAfpmt1 mutant showed that a lack of AfPmt1p results in sensitivity to elevated temperature and defects in growth and cell wall integrity, thereby affecting cell morphology, conidium formation, and germination. In a mouse model, Afpmt1 was not required for the virulence of A. fumigatus under the experimental conditions used.

scholarly journals Role of the Guanine Nucleotide Exchange Factor Rom2 in Cell Wall Integrity Maintenance of Aspergillus fumigatus

2012 ◽  
Vol 12 (2) ◽  
pp. 288-298 ◽  
Author(s):  
Sweta Samantaray ◽  
Michael Neubauer ◽  
Christoph Helmschrott ◽  
Johannes Wagener
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Guanine Nucleotide Exchange Factor ◽  
Cell Wall Integrity ◽  
Guanine Nucleotide ◽  
Nucleotide Exchange ◽  
Content Type ◽  
Guanine Nucleotide Exchange ◽  
Stress Sensors ◽  
Nucleotide Exchange Factor

ABSTRACTAspergillus fumigatusis a mold and the causal agent of invasive aspergillosis, a systemic disease with high lethality. Recently, we identified and functionally characterized three stress sensors implicated in the cell wall integrity (CWI) signaling of this pathogen, namely, Wsc1, Wsc3, and MidA. Here, we functionally characterize Rom2, a guanine nucleotide exchange factor with essential function for the cell wall integrity ofA. fumigatus. A conditionalrom2mutant has severe growth defects under repressive conditions and incorporates all phenotypes of the three cell wall integrity sensor mutants, e.g., the echinocandin sensitivity of the Δwsc1mutant and the Congo red, calcofluor white, and heat sensitivity of the ΔmidAmutant. Rom2 interacts with Rho1 and shows a similar intracellular distribution focused at the hyphal tips. Our results place Rom2 between the cell surface stress sensors Wsc1, Wsc3, MidA, and Rho1 and their downstream effector mitogen-activated protein (MAP) kinase module Bck1-Mkk2-MpkA.

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