scholarly journals Geranylgeranyltransferase Cwg2-Rho4/Rho5 module is implicated in the Pmk1 MAP kinase-mediated cell wall integrity pathway in fission yeast

2015 ◽  
Vol 20 (4) ◽  
pp. 310-323 ◽  
Author(s):  
Akira Doi ◽  
Ayako Kita ◽  
Yuki Kanda ◽  
Takaya Uno ◽  
Keita Asami ◽  
...  
Keyword(s):  
Cell Wall ◽  
Map Kinase ◽  
Fission Yeast ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase C

PLoS Genetics ◽  
2014 ◽  
Vol 10 (9) ◽  
pp. e1004582 ◽  
Author(s):  
Ines Teichert ◽  
Eva Katharina Steffens ◽  
Nicole Schnaß ◽  
Benjamin Fränzel ◽  
Christoph Krisp ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Map Kinase ◽  
Scaffold Protein ◽  
Cell Wall Integrity ◽  
Activator Protein ◽  
Kinase C ◽  
Cell Wall Integrity Pathway

scholarly journals The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae

2003 ◽  
Vol 49 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Helene Martin-Yken ◽  
Adilia Dagkessamanskaia ◽  
Fadi Basmaji ◽  
Arnaud Lagorce ◽  
Jean Francois
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Map Kinase ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis

2018 ◽  
Vol 29 (19) ◽  
pp. 2259-2279 ◽  
Author(s):  
Yi-Hua Zhu ◽  
Joanne Hyun ◽  
Yun-Zu Pan ◽  
James E. Hopper ◽  
Josep Rizo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Cell Types ◽  
Cell Wall Integrity ◽  
Uncharacterized Protein ◽  
Division Plane ◽  
Division Site ◽  
Protein Receptor ◽  
Cell Wall Integrity Pathway

Cytokinesis is a complicated yet conserved step of the cell-division cycle that requires the coordination of multiple proteins and cellular processes. Here we describe a previously uncharacterized protein, Ync13, and its roles during fission yeast cytokinesis. Ync13 is a member of the UNC-13/Munc13 protein family, whose animal homologues are essential priming factors for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex assembly during exocytosis in various cell types, but no roles in cytokinesis have been reported. We find that Ync13 binds to lipids in vitro and dynamically localizes to the plasma membrane at cell tips during interphase and at the division site during cytokinesis. Deletion of Ync13 leads to defective septation and exocytosis, uneven distribution of cell-wall enzymes and components of cell-wall integrity pathway along the division site and massive cell lysis during cell separation. Interestingly, loss of Ync13 compromises endocytic site selection at the division plane. Collectively, we find that Ync13 has a novel function as an UNC-13/Munc13 protein in coordinating exocytosis, endocytosis, and cell-wall integrity during fission yeast cytokinesis.

Pneumocystis carinii BCK1 Complements the Saccharomyces cerevisiae Cell Wall Integrity Pathway

2003 ◽  
Vol 50 (s1) ◽  
pp. 676-677 ◽  
Author(s):  
PAWAN K. VOHRA ◽  
THEODORE J. KOTTOM ◽  
ANDREW H. LIMPER ◽  
CHARLES F. THOMAS
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Pneumocystis Carinii ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals Protein O-mannosylation is crucial for cell wall integrity, septation and viability in fission yeast

2005 ◽  
Vol 57 (1) ◽  
pp. 156-170 ◽  
Author(s):  
Tobias Willer ◽  
Martin Brandl ◽  
Matthias Sipiczki ◽  
Sabine Strahl
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Cell Wall Integrity

scholarly journals Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence

mBio ◽  
2020 ◽  
Vol 11 (5) ◽  
Author(s):  
Aílton Pereira da Costa Filho ◽  
Guilherme Thomaz Pereira Brancini ◽  
Patrícia Alves de Castro ◽  
Clara Valero ◽  
Jaire Alves Ferreira Filho ◽  
...  
Keyword(s):  
Cell Wall ◽  
G Protein ◽  
Galleria Mellonella ◽  
G Protein Coupled Receptors ◽  
Cell Wall Integrity ◽  
Fungal Development ◽  
Content Type ◽  
Cell Wall Integrity Pathway ◽  
Mycotoxin Biosynthesis ◽  
G Protein Coupled

ABSTRACT G-protein coupled receptors (GPCRs) are extracellular signaling receptors that sense environmental cues. Fungi sense their environment primarily through GPCR-mediated signaling pathways, which, in turn, regulate fungal development, metabolism, virulence, and mycotoxin biosynthesis. Aspergillus fumigatus is an important human pathogen that causes aspergillosis, a heterogeneous group of diseases that present a wide range of clinical manifestations. Here, we investigate in detail the role of the GPCRs GprM and GprJ in growth and gene expression. GprM and GprJ are important for melanin production and the regulation of the cell wall integrity (CWI) pathway. Overexpression of gprM and gprJ causes a 20 and 50% reduction in growth rate compared to the wild-type (WT) strain and increases sensitivity to cell wall-damaging agents. Phosphorylation of the CWI protein kinase MpkA is increased in the ΔgprM and ΔgprJ strains and decreased in the overexpression mutants compared to the WT strain. Furthermore, differences in cell wall polysaccharide concentrations and organization were observed in these strains. Transcriptome sequencing suggests that GprM and GprJ negatively regulate genes encoding secondary metabolites (SMs). Mass spectrometry analysis confirmed that the production of fumagillin, pyripyropene, fumigaclavine C, fumiquinazoline, and fumitremorgin is reduced in the ΔgprM and ΔgprJ strains, at least partially through the activation of MpkA. Overexpression of grpM also resulted in the regulation of many transcription factors, with AsgA predicted to function downstream of GprM and MpkA signaling. Finally, we show that the ΔgprM and ΔgprJ mutants are reduced in virulence in the Galleria mellonella insect model of invasive aspergillosis. IMPORTANCE A. fumigatus is the main etiological agent of invasive pulmonary aspergillosis, a life-threatening fungal disease that occurs in severely immunocompromised humans. Withstanding the host environment is essential for A. fumigatus virulence, and sensing of extracellular cues occurs primarily through G-protein coupled receptors (GPCRs) that activate signal transduction pathways, which, in turn, regulate fungal development, metabolism, virulence, and mycotoxin biosynthesis. The A. fumigatus genome encodes 15 putative classical GPCRs, with only three having been functionally characterized to date. In this work, we show that the two GPCRs GprM and GprJ regulate the phosphorylation of the mitogen-activated protein kinase MpkA and thus control the regulation of the cell wall integrity pathway. GprM and GprJ are also involved in the regulation of the production of the secondary metabolites fumagillin, pyripyropene, fumigaclavine C, fumiquinazoline, melanin, and fumitremorgin, and this regulation partially occurs through the activation of MpkA. Furthermore, GprM and GprJ are important for virulence in the insect model Galleria mellonella. This work therefore functionally characterizes two GPCRs and shows how they regulate several intracellular pathways that have been shown to be crucial for A. fumigatus virulence.

The effect of the cwf14 gene of fission yeast on cell wall integrity is associated with rho1

2016 ◽  
Vol 54 (2) ◽  
pp. 98-105
Author(s):  
Dong-Uk Kim ◽  
Shinae Maeng ◽  
Hyemi Lee ◽  
Miyoung Nam ◽  
Sook-Jeong Lee ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fission Yeast ◽  
Cell Wall Integrity
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