An optimal window for the fabrication of Edible Polyelectrolyte Complex Nanotubes (EPCNs) from bovine serum albumin (BSA) and sodium alginate

2018 ◽  
Vol 77 ◽  
pp. 336-346 ◽  
Author(s):  
L. Maldonado ◽  
J. Kokini
2020 ◽  
Vol 137 (34) ◽  
pp. 49002 ◽  
Author(s):  
Violeta Paşcalău ◽  
Mihaela Tertis ◽  
Emoke Pall ◽  
Maria Suciu ◽  
Traian Marinca ◽  
...  

1998 ◽  
Vol 12 (2) ◽  
pp. 127-132 ◽  
Author(s):  
Susann Neiser ◽  
Kurt I. Draget ◽  
Olav Smidsrød

2012 ◽  
Vol 625 ◽  
pp. 206-209
Author(s):  
Wen Kui Cui ◽  
Kong Yin Zhao ◽  
Jun Fu Wei ◽  
Guo Xiang Cheng

Calcium polyacrylate/alginate (CPA/A) hybrid polymer microspheres with the imprinting of bovine serum albumin (BSA) were prepared via Ca2+ crosslinking of sodium polyacrylate (SPA), BSA and sodium alginate (SA) in inverse suspension. The specific rebinding properties of BSA imprinted hydrogel microspheres (CPA/A MIPMs) were investigated by controlling pH value from the viewpoint of adjusting the process of gelling, removing template and rebinding. The optimized pH values in gelling, removing template and rebinding process was 4.1, 8.3 and 4.2, respectively.


Author(s):  
G. D. Gagne ◽  
M. F. Miller

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.


Sign in / Sign up

Export Citation Format

Share Document