Stability of some atypical antipsychotics in human plasma, haemolysed whole blood, oral fluid, human serum and calf serum

2013 ◽  
Vol 229 (1-3) ◽  
pp. 151-156 ◽  
Author(s):  
Danielle S. Fisher ◽  
Suzanne J. Partridge ◽  
Simon A. Handley ◽  
Robert J. Flanagan
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Whole Blood ◽  
Oral Fluid ◽  
Atypical Antipsychotics ◽  
Calf Serum

LC–MS/MS of some atypical antipsychotics in human plasma, serum, oral fluid and haemolysed whole blood

2013 ◽  
Vol 229 (1-3) ◽  
pp. 145-150 ◽  
Author(s):  
Danielle S. Fisher ◽  
Suzanne J. Partridge ◽  
Simon A. Handley ◽  
Lewis Couchman ◽  
Phillip E. Morgan ◽  
...  
Keyword(s):  
Human Plasma ◽  
Whole Blood ◽  
Oral Fluid ◽  
Atypical Antipsychotics

Plasma Thromboplastin Component (Christmas Factor, Factor IX) Levels in Stored Human Blood and Plasma

1960 ◽  
Vol 04 (03) ◽  
pp. 376-388 ◽  
Author(s):  
J Dieter Geratz ◽  
John B. Graham
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Whole Blood ◽  
Room Temperature ◽  
Close Agreement ◽  
Factor Ix ◽  
Deficient Patient ◽  
Glass Tubes ◽  
Disodium Hydrogen Citrate ◽  
Bank Blood

Summary1. PTC activity was assayed in 26 units of human plasma prepared from whole blood stored for 3 weeks at 4° C. The plasma had been frozen and stored at — 20° C for additional periods ranging from a few days to 4 months. High PTC activity was still present in the plasma at the end of this period, the activity averaging 95% of normal.2. The PTC activity of 19 samples of “reclaimed“ plasma stored for an additional 6 months at — 20° C decreased by an average of 23%. This decrease was statistically significant.3. Liquid plasma kept at room temperature for 5½—7½ months contained no PTC activity.4. Lyophilized plasma stored at room temperature for 6—8 years contained an average of 30% PTC activity. Lyophilized plasma stored at — 20° C for 4 years contained 68% PTC activity.5. ACD and disodium hydrogen citrate anticoagulant solutions served equally well in preserving PTC activity in whole blood stored in glass tubes over a period of 3 weeks at 4° C.6. “Reclaimed“ plasma from outdated bank blood provided effective hemostasis in two operations for the removal of 20 teeth from a severely PTC-deficient patient.7. The high PTC activity of “reclaimed“ plasma was confirmed by the close agreement between the PTC level expected in a PTC deficient patient after transfusion of such plasma and that observed.

Factor V from Human Plasma

1961 ◽  
Vol 05 (01) ◽  
pp. 001-020
Author(s):  
Douglas M. Surgenor ◽  
Nancy A. Wilson ◽  
Anne S. Henry
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Kinetic Data ◽  
Human Factor ◽  
Kinetic Studies ◽  
Partial Purification ◽  
Factor V ◽  
Two Stage ◽  
Plasma Factor ◽  
Tissue Thromboplastin

SummaryA method is described for the partial purification of a human plasma factor which accelerates the conversion of prothrombin to thrombin in the presence of tissue thromboplastin. This factor may be dried from the frozen state, and may be kept in stable dry form for long periods of time. The quantitative assay of this activity is done in a classical two-stage prothrombin system using tissue thromboplastin and calcium. From its properties, it is concluded that this activity corresponds to factor V, labile factor and plasma Ac-globulin.Chemical and kinetic studies reveal that human factor V is active in plasma and is destroyed by thrombin. Human serum has little or no factor V activity.These results thus fail to support the postulated activation of factor V during clotting. All of the kinetic data are consistent with an enzymatic role for factor V in the formation of tissue prothrombin activator (thromboplastin).

Abstract 238: Incubation of MDCO216 With Human Serum or Plasma Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Increases Prebeta-1 HDL and Causes a Shift From Small to Large Alpha HDL Particles

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Herman J Kempen ◽  
Dorota B Schranz ◽  
Bela Asztalos ◽  
Elias Jeyarajah ◽  
James Otvos ◽  
...  
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Cholesterol Efflux ◽  
Time Dependent ◽  
Plaque Burden ◽  
Strong Increase ◽  
2D Electrophoresis ◽  
Cholesterol Efflux Capacity ◽  
J774 Cells ◽  
Synergistic Increase

MDCO216 is a complex of dimeric apoA-IMilano and POPC, shown to reduce atherosclerotic plaque burden. Here we studied the effect of incubation of human plasma or serum with MDCO216 on cholesterol efflux capacity from J774 cells, on prebeta-1 HDL and various HDL subfractions. At clinically relevant concentrations MDCO216 by itself markedly stimulates global and ABCA1-mediated cholesterol efflux. When incubated with human serum a time-dependent synergistic increase of the ABCA1 mediated efflux capacity is observed. Using the Sekisui ELISA for prebeta-1 HDL, MDCO216 itself is poorly detected. Prebeta-1 HDL is rapidly lost when human plasma alone is incubated at 37 o C. However, incubation of human plasma with MDCO216 at 37 o C causes a robust generation of new prebeta-1 HDL. 2D-Electrophoresis followed by immunoblotting with a general apoA-I antibody that also detects apoA-IM confirms the increase in prebeta-1 HDL (having a different mobility than pure MDCO216 particles), and shows a concomitant disappearance of alpha-3 HDL, alpha-4 HDL and MDCO216, and an increase in alpha-1 and alpha-2 HDL. NMR analysis of plasma incubated with MDCO216 at 47 o C confirms very rapid disappearance of small HDL and increase of medium and large HDL particles. In conclusion, incubation of human plasma or serum with MDCO216 strongly enhances ABCA1-mediated cholesterol efflux capacity, causes a strong increase of prebeta-1 HDL and drastically changes HDL subfraction distribution, consistent with anti-atherosclerotic activity.

Phthalate Metabolites in Paired Human Serum and Whole Blood

2021 ◽  
Author(s):  
Weili Mao ◽  
Nan Zhao ◽  
Meirong Zhao ◽  
jin hangbiao
Keyword(s):  
Human Serum ◽  
Whole Blood ◽  
Phthalate Metabolites
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