Understanding Physical Developer (PD): Part II – Is PD targeting eccrine constituents?

2015 ◽  
Vol 257 ◽  
pp. 488-495 ◽  
Author(s):  
Mackenzie de la Hunty ◽  
Sébastien Moret ◽  
Scott Chadwick ◽  
Chris Lennard ◽  
Xanthe Spindler ◽  
...  
Keyword(s):  
Physical Developer

Examination of the Steps Leading up to the Physical Developer Process for Developing Fingerprints

2007 ◽  
Vol 52 (2) ◽  
pp. 320-329 ◽  
Author(s):  
Jeffrey Daniel Wilson ◽  
Antonio A. Cantu ◽  
George Antonopoulos ◽  
Marc J. Surrency
Keyword(s):  
Physical Developer

Understanding physical developer (PD): Part I – Is PD targeting lipids?

2015 ◽  
Vol 257 ◽  
pp. 481-487 ◽  
Author(s):  
Mackenzie de la Hunty ◽  
Sébastien Moret ◽  
Scott Chadwick ◽  
Chris Lennard ◽  
Xanthe Spindler ◽  
...  
Keyword(s):  
Physical Developer

scholarly journals A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction.

1989 ◽  
Vol 37 (10) ◽  
pp. 1563-1565 ◽  
Author(s):  
I Merchenthaler ◽  
J Stankovics ◽  
F Gallyas
Keyword(s):  
Physical Development ◽  
New Technique ◽  
Step Method ◽  
Substrate Solution ◽  
Peroxidase Reaction ◽  
Additional Step ◽  
Highly Sensitive ◽  
One Step ◽  
A New Technique ◽  
Physical Developer

We have developed a new technique which makes silver intensification of the oxidatively polymerized diaminobenzidine (DAB), the endproduct of peroxidase reaction, less laborious without any loss in selectivity or sensitivity. The new technique is based on two strategies: (a) increasing the argyrophilia of the DAB by modifying its polymerization with Ni ions, and (b) decreasing tissue argyrophila by using a mildly acidic physical developer instead of the alkaline one previously presented. Because the nickel modification takes place in the DAB substrate solution, i.e., in the final step of the peroxidase reaction, only one additional step, the physical development, must be carried out if intensification is needed.

scholarly journals Replacing Synperonic® N in the Physical Developer Fingermark Visualisation Process: Pseudo-Operational Trial and Parameter Studies

2021 ◽  
pp. 110916
Author(s):  
Emily M. Cartledge ◽  
Zi Ying Guo ◽  
Stephen M. Bleay ◽  
Vaughn G. Sears ◽  
Laura J. Hussey
Keyword(s):  
Physical Developer

scholarly journals Experiments on the Mechanism of Silver Staining

1955 ◽  
Vol s3-96 (33) ◽  
pp. 103-115
Author(s):  
A. PETERS
Keyword(s):  
Silver Staining ◽  
Protective Action ◽  
The Other ◽  
Physical Developer ◽  
Nerve Staining

The effect of a series of photographic developers on the final silver-staining picture has been investigated. Ten common developers were used, but of these only hydroquinone, chloroquinol, pyrogallol, and p-aminophenol, were found to be of general use. The other developers were either so weak in their action that the final staining was light and incomplete, or so powerful that a differentiated nerve staining was not produced. For silver staining to be effected nuclei of reduced silver should be present in the section. These nuclei act as centres for the deposition of additional silver reduced by the developer; the additional silver may either be derived from that combined with the sections during impregnation or from the developing solution itself. Whether or ot the additional silver is deposited in such a way as to produce differentiated nerve staining depends on the properties of the developer and on the composition of the developing solution. The redox- and ‘bromide’-potentials, the sulphite and hydrogen n concentrations in the developing solution, and the protective action of the tissue components of the section all play a part in determining the final staining picture. A new glycine-containing physical developer and a gold thiocyanate physical developer are described.

Electron Microscopic Radioautography: The Sites of Nuclear RNA Synthesis in Early Embryonic Cells

1968 ◽  
Vol 26 ◽  
pp. 4-5
Author(s):  
Shuichi Karasaki
Keyword(s):  
Rna Synthesis ◽  
Sea Water ◽  
Ultrastructural Organization ◽  
Embryonic Cells ◽  
Electron Microscopic ◽  
Interphase Nuclei ◽  
Thin Sections ◽  
Arbacia Punctulata ◽  
The Relationship ◽  
Physical Developer

The relationship between RNA synthesis and ultrastructural organization of chromatin fibrils was examined in the interphase nuclei of the sea urchin embryos (Arbacia punctulata). The embryos at various stages of development were treated for 1 or 3 hours in sea water with 50 μC/ml of 3H-5-uridine. The samples were fixed in OSO4 or glutaraldehyde (GTA) and embedded in Epon or glycol methacrylate (GMA). Radioautographs were made by applying a thin film of Ilford L4 emulsion directly from an expandable wire loop to thin sections mounted on grids. Following exposure of 10 to 20 weeks, they were developed in Microdol-X or physical developer.In the cleavage and early blastula embryos, each interphase nucleus contains diffusely distributed chromatin and shows only very low incorporation of 3H-uridine into RNA (Fig.1). During these periods, many nucleolus-like bodies occur within the nucleus although they are not labeled with the isotope.

Experiments on the Mechanism of Silver Staining

1955 ◽  
Vol s3-96 (35) ◽  
pp. 301-315
Author(s):  
A. PETERS
Keyword(s):  
Silver Staining ◽  
Autocatalytic Reaction ◽  
Solution Temperature ◽  
Quantitative Results ◽  
Physical Developer

The quantitative aspects of silver staining of sections have been investigated with radioactive silver (Ag111). The concentrations of reducible silver, developed silver, and silver nuclei in the sections were determined, but it is doubtful if the values obtained for silver nuclei are significant. All three forms of silver increased with pH, time, and the concentration of silver in the impregnating solution. Temperature of impregnation had little effect on the uptake of reducible silver, but increased the developed silver, presumably by increasing the silver nuclei. An increase in the temperature of a hydroquinone-sulphite developer increased the amount of reducible silver reduced by the developer. The deposition of silver by a glycine physical developer was shown to follow a curve which was reasonably consistent with the assumption of a typical autocatalytic reaction. The uptake of silver by non-nervous tissues provided evidence that the process is not specific for nerves; the final specificity of staining is determined during development. The quantitative results are consistent with the hypothesis that the histidine in the sections is responsible for the combination of reducible silver.

scholarly journals An immunogold-silver staining method for detection of cell-surface antigens in light microscopy.

1986 ◽  
Vol 34 (7) ◽  
pp. 935-939 ◽  
Author(s):  
M De Waele ◽  
J De Mey ◽  
W Renmans ◽  
C Labeur ◽  
P Reynaert ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Light Microscopy ◽  
Silver Staining ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
T Cell Subsets ◽  
Staining Procedure ◽  
Cell Surface Antigens ◽  
Physical Developer

An immunogold-silver staining technique for detection of cell-surface antigens in cell suspensions was developed. Leukocyte cell suspensions were first incubated with monoclonal antibodies directed against cell-surface antigens and then with colloidal gold-labeled goat anti-mouse antibodies. Cytocentrifuge preparations of the cell suspensions were immersed in a physical developer containing silver lactate and hydroquinone as reducing substance. The preparations were then counterstained and mounted. In light microscopy, cells reacting with the monoclonal antibodies showed dark granules on their surface membrane. An optimal morphology, as revealed by a May-Grünwald-Giemsa counterstain, permitted accurate cell identification. The labeling was influenced by the gold particle diameter and the concentration of the gold reagents, by the duration of incubation in the physical developer, and by the composition and temperature of this medium. The T-cell subsets enumerated with this method in the peripheral blood of normal adults were identical to those found with other methods. The sensitivity of the technique was comparable with that of immunofluorescence microscopy. This immunogold-silver staining procedure proved to be a reliable tool for detection of cell-surface antigens in light microscopy.

Modifications to the Silver Physical Developer

2003 ◽  
Vol 48 (5) ◽  
pp. 2003044 ◽  
Author(s):  
David Burow ◽  
Donald Seifert ◽  
Antonio A. Cantu
Keyword(s):  
Physical Developer
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