scholarly journals A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction.

1989 ◽  
Vol 37 (10) ◽  
pp. 1563-1565 ◽  
Author(s):  
I Merchenthaler ◽  
J Stankovics ◽  
F Gallyas

We have developed a new technique which makes silver intensification of the oxidatively polymerized diaminobenzidine (DAB), the endproduct of peroxidase reaction, less laborious without any loss in selectivity or sensitivity. The new technique is based on two strategies: (a) increasing the argyrophilia of the DAB by modifying its polymerization with Ni ions, and (b) decreasing tissue argyrophila by using a mildly acidic physical developer instead of the alkaline one previously presented. Because the nickel modification takes place in the DAB substrate solution, i.e., in the final step of the peroxidase reaction, only one additional step, the physical development, must be carried out if intensification is needed.

Endoscopy ◽  
1998 ◽  
Vol 30 (08) ◽  
pp. 730-733 ◽  
Author(s):  
P. Vilmann ◽  
S. Hancke ◽  
T. Pless ◽  
J. D. Schell-Hincke ◽  
F. W. Henriksen

2019 ◽  
Vol 11 (22) ◽  
pp. 2937-2942 ◽  
Author(s):  
Mingsong Zhu ◽  
Yurui Jia ◽  
Lizhi Peng ◽  
Jifu Ma ◽  
Xiangru Li ◽  
...  

A lateral flow immunoassay was developed to improve clinical care compared with conventional brucellosis screening methods. Detection is dual-color in format using dyed, carboxyl-functionalized latex microspheres synthesized with a one-step method.


2017 ◽  
Vol 53 (37) ◽  
pp. 5121-5124 ◽  
Author(s):  
B. Fortuni ◽  
Y. Fujita ◽  
M. Ricci ◽  
T. Inose ◽  
R. Aubert ◽  
...  

A one-step method to form gold nanostars on a polydimethylsiloxane film is proposed for highly-sensitive SERS substrates.


1991 ◽  
Vol 5 (1-4) ◽  
pp. 253-255 ◽  
Author(s):  
Giorgio Sberveglieri ◽  
Silvio Groppelli ◽  
Paolo Nelli ◽  
Alberto Camanzi

1995 ◽  
Vol 149 ◽  
pp. 33-37
Author(s):  
K. Taylor ◽  
N.G. Douglas

AbstractPlanetary Nebulae are proving to be powerful tertiary distance indicators as well as sensitive probes of a galaxy’s velocity field and mass distribution. We present a new technique for determining the positions, luminosities and radial velocities of the PNe in an external galaxy which may enable a one-step measurement of all these parameters.


2019 ◽  
Vol 7 (11) ◽  
pp. 6401-6410 ◽  
Author(s):  
Jizhen Zhang ◽  
Shayan Seyedin ◽  
Si Qin ◽  
Peter A. Lynch ◽  
Zhiyu Wang ◽  
...  

Here, we report a one-step method to produce highly conducting poly(3,4-ethylenedioxythiophene):poly(4-styrenesulfonate) fibers that enables applications in fast response and highly sensitive touch sensors, body moisture monitoring, and long fiber-shaped supercapacitors.


Author(s):  
Gianluca Frasca-Caccia ◽  
Peter E. Hydon

AbstractThis paper introduces a new symbolic-numeric strategy for finding semidiscretizations of a given PDE that preserve multiple local conservation laws. We prove that for one spatial dimension, various one-step time integrators from the literature preserve fully discrete local conservation laws whose densities are either quadratic or a Hamiltonian. The approach generalizes to time integrators with more steps and conservation laws of other kinds; higher-dimensional PDEs can be treated by iterating the new strategy. We use the Boussinesq equation as a benchmark and introduce new families of schemes of order two and four that preserve three conservation laws. We show that the new technique is practicable for PDEs with three dependent variables, introducing as an example new families of second-order schemes for the potential Kadomtsev–Petviashvili equation.


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