In Vivo Melanoma Imaging on Living Mice Using Dynamic Nuclear Polarization Magnetic Resonance Imaging

AbstractMagnetic Resonance Imaging combined with hyperpolarized 13C-labelled metabolic contrast agents produced via dissolution Dynamic Nuclear Polarization can, non-invasively and in real-time, report on tissue specific aberrant metabolism. However, hyperpolarization equipment is expensive, technically demanding and needs to be installed on-site for the end-user. In this work, we provide a robust methodology that allows remote production of the hyperpolarized 13C-labelled metabolic contrast agents. The methodology, built on photo-induced thermally labile radicals, allows solid sample extraction from the hyperpolarization equipment and several hours’ lifetime of the 13C-labelled metabolic contrast agents at appropriate storage/transport conditions. Exemplified with [U-13C, d7]-D-glucose, we remotely produce hyperpolarized 13C-labelled metabolic contrast agents and generate above 10,000-fold liquid-state Magnetic Resonance signal enhancement at 9.4 T, keeping on-site only a simple dissolution device.

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13C-labeled biochemical probes for the study of cancer metabolism with dynamic nuclear polarization-enhanced magnetic resonance imaging

Cancer & Metabolism ◽

10.1186/s40170-015-0136-2 ◽

2015 ◽

Vol 3 (1) ◽

Cited By ~ 21

Author(s):

Lucia Salamanca-Cardona ◽

Kayvan R. Keshari

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Dynamic Nuclear Polarization ◽

Nuclear Polarization ◽

Cancer Metabolism ◽

Resonance Imaging

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Monitoring urea transport in rat kidney in vivo using hyperpolarized 13C magnetic resonance imaging

AJP Renal Physiology ◽

10.1152/ajprenal.00640.2011 ◽

2012 ◽

Vol 302 (12) ◽

pp. F1658-F1662 ◽

Cited By ~ 30

Author(s):

Cornelius von Morze ◽

Robert A. Bok ◽

Jeff M. Sands ◽

John Kurhanewicz ◽

Daniel B. Vigneron

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Nuclear Polarization ◽

Collecting Duct ◽

Rat Kidney ◽

Resonance Imaging ◽

Hyperpolarized 13C ◽

New Methods ◽

Medullary Collecting Duct

Urea functions as a key osmolyte in the urinary concentrating mechanism of the inner medulla. The urea transporter UT-A1 is upregulated by antidiuretic hormone, facilitating faster equilibration of urea between the lumen and interstitium of the inner medullary collecting duct, resulting in the formation of more highly concentrated urine. New methods in dynamic nuclear polarization, providing ∼50,000-fold enhancement of nuclear magnetic resonance signals in the liquid state, offer a novel means to monitor this process in vivo using magnetic resonance imaging. In this study, we detected significant signal differences in the rat kidney between acute diuretic and antidiuretic states, using dynamic 13C magnetic resonance imaging following a bolus infusion of hyperpolarized [13C]urea. More rapid medullary enhancement was observed under antidiuresis, consistent with known upregulation of UT-A1.

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