Development of a one-tube extraction and amplification method for DNA analysis of sperm and epithelial cells recovered from forensic samples by laser microdissection

2012 ◽  
Vol 6 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Melanie Meredith ◽  
Jo-Anne Bright ◽  
Sarah Cockerton ◽  
Sue Vintiner
Keyword(s):  
Epithelial Cells ◽  
Dna Analysis ◽  
Laser Microdissection ◽  
Amplification Method ◽  
Forensic Samples

scholarly journals Laser Microdissection for Rapid Separation of Spermatozoa or Male Epithelial Cells from Cell Mixtures from Sexual Assault Evidence, and Other Applications for Forensic Analysis

2006 ◽  
Vol 12 (S02) ◽  
pp. 332-333
Author(s):  
A Lee
Keyword(s):  
Sexual Assault ◽  
Epithelial Cells ◽  
Laser Microdissection ◽  
Forensic Analysis ◽  
Rapid Separation

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

scholarly journals Innocent until proven guilty: Privacy-preserving search over a central CODIS criminal database from the field

2020 ◽  
Author(s):  
Jacob A. Blindenbach ◽  
Karthik A. Jagadeesh ◽  
Gill Bejerano ◽  
David J. Wu
Keyword(s):  
Justice System ◽  
Dna Analysis ◽  
Crime Scene ◽  
The United States ◽  
Dna Index ◽  
Dna Profile ◽  
Cryptographic Algorithm ◽  
Forensic Samples ◽  
Dna Profiles ◽  
Database Match

AbstractThe presumption of innocence (i.e., the principle that one is considered innocent until proven guilty) is a cornerstone of the criminal justice system in many countries, including the United States. DNA analysis is an important tool for criminal investigations1. In the U.S. alone, it has already aided in over half a million investigations using the Combined DNA Index System (CODIS) and associated DNA databases2. CODIS includes DNA profiles of crime scene forensic samples, convicted offenders, missing persons and more. The CODIS framework is currently used by over 50 other countries3 including much of Europe, Canada, China and more. During investigations, DNA samples can be collected from multiple individuals who may have had access to, or were found near a crime scene, in the hope of finding a single criminal match4. Controversially, CODIS samples are sometimes retained from adults and juveniles despite not yielding any database match4–6. Here we introduce a cryptographic algorithm that finds any and all matches of a person’s DNA profile against a CODIS database without revealing anything about the person’s profile to the database provider. With our protocol, matches are immediately identified as before; however, individuals who do not match anything in the database retain their full privacy. Our novel algorithm runs in 40 seconds on a CODIS database of 1,000,000 entries, enabling its use to privately screen potentially-innocent suspects even in the field.

Contact-Free Isolation of Sperm and Epithelial Cells by Laser Microdissection and Pressure Catapulting

2005 ◽  
Vol 1 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Stephan Seidl ◽  
Renate Burgemeister ◽  
Roland Hausmann ◽  
Peter Betz ◽  
Thomas Lederer
Keyword(s):  
Epithelial Cells ◽  
Laser Microdissection ◽  
Contact Free

Laser Microdissection Separation of Pure Spermatozoa from Epithelial Cells for Short Tandem Repeat Analysis*

2006 ◽  
Vol 51 (4) ◽  
pp. 748-757 ◽  
Author(s):  
Christine T. Sanders ◽  
Nick Sanchez ◽  
Jack Ballantyne ◽  
Daniel A. Peterson
Keyword(s):  
Epithelial Cells ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Laser Microdissection ◽  
Repeat Analysis ◽  
Short Tandem

scholarly journals Past, Present, and Future of DNA Typing for Analyzing Human and Non-Human Forensic Samples

2021 ◽  
Vol 9 ◽  
Author(s):  
Deidra Jordan ◽  
DeEtta Mills
Keyword(s):  
Capillary Electrophoresis ◽  
Genetic Analysis ◽  
Dna Analysis ◽  
Dna Typing ◽  
Single Strand Conformation Polymorphism ◽  
Forensic Analysis ◽  
Forensic Dna ◽  
Forensic Dna Analysis ◽  
Forensic Samples ◽  
Next Generation Sequencing Ngs

Forensic DNA analysis has vastly evolved since the first forensic samples were evaluated by restriction fragment length polymorphism (RFLP). Methodologies advanced from gel electrophoresis techniques to capillary electrophoresis and now to next generation sequencing (NGS). Capillary electrophoresis was and still is the standard method used in forensic analysis. However, dependent upon the information needed, there are several different techniques that can be used to type a DNA fragment. Short tandem repeat (STR) fragment analysis, Sanger sequencing, SNapShot, and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) are a few of the techniques that have been used for the genetic analysis of DNA samples. NGS is the newest and most revolutionary technology and has the potential to be the next standard for genetic analysis. This review briefly encompasses many of the techniques and applications that have been utilized for the analysis of human and nonhuman DNA samples.

scholarly journals Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria

2008 ◽  
Vol 74 (13) ◽  
pp. 4231-4235 ◽  
Author(s):  
Stefanie Gloess ◽  
Hans-Peter Grossart ◽  
Martin Allgaier ◽  
Stefan Ratering ◽  
Michael Hupfer
Keyword(s):  
Activated Sludge ◽  
Lake Sediment ◽  
Environmental Samples ◽  
Dna Analysis ◽  
Laser Microdissection ◽  
Taxonomic Identification ◽  
Phylogenetic Characterization ◽  
Uncultured Bacteria ◽  
Novel Approach

ABSTRACT Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.

scholarly journals Sequential changes of bronchial epithelial cells in heavy smokers. Fluorescence cytophotometric nuclear DNA analysis.

Haigan ◽  
1986 ◽  
Vol 26 (1) ◽  
pp. 11-18
Author(s):  
Masashiro Yamazaki ◽  
Masayoshi Takahashi ◽  
Masahiko Fujii ◽  
Kei Hagiwara ◽  
Kinya Hayakawa
Keyword(s):  
Epithelial Cells ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Heavy Smokers
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