Genetic population studies on 15 NGM™ STR loci in central Poland population

2012 ◽  
Vol 6 (4) ◽  
pp. e119-e120 ◽  
Author(s):  
Maciej Jedrzejczyk ◽  
Renata Jacewicz ◽  
Stefan Szram ◽  
Jaroslaw Berent
Zootaxa ◽  
2021 ◽  
Vol 4948 (2) ◽  
pp. 151-183
Author(s):  
DANIEL CHIRIVI JOYA

Mexico is the country with the largest diversity of the genus Phrynus (Amblypygi: Phrynidae); however, many species could remain hidden due to the conservative morphology of the group. Two of the Mexican species, P. operculatus, and P. jalisco show enough variation, between populations from different localities, to be considered as possible species complexes. The scarce taxonomic information of these species and the few analyses of their variation could cause misidentifications and sub estimation of the diversity. Specimens morphologically similar to P. operculatus from different Mexican localities were analyzed, using characters from the male and female genitals. Four new species are described, presenting illustrations of SEM for the male genitals. Details of the morphologic variation in this group of species are discussed, as well as the usefulness of sexual characters like the size proportions, and sclerotization in the female gonopods, and ornaments in males, to improve the diagnoses of the Mexican Phrynus. The known distribution of P. operculatus is large in comparison with those of the other species, after the result of this research, the possibility that it is a larger species complex is evident, thus, it is necessary to increase the morphologic and genetic population studies in this group. 


2013 ◽  
Vol 7 (3) ◽  
pp. e56-e58 ◽  
Author(s):  
Panayotis Katsaloulis ◽  
Konstantina Tsekoura ◽  
Maria Vouropoulou ◽  
Penelope Miniati

2002 ◽  
Vol 130 (1) ◽  
pp. 55-57 ◽  
Author(s):  
Piotr Kuzniar ◽  
Ireneusz Soltyszewski ◽  
Rafal Ploski

2009 ◽  
Vol 125 (1) ◽  
pp. 111-119 ◽  
Author(s):  
L. H. P. van de Goor ◽  
M. T. Koskinen ◽  
W. A. van Haeringen
Keyword(s):  

2019 ◽  
Vol 27 (3) ◽  
pp. 207-211
Author(s):  
Renata Neves Biancalana ◽  
Fabio Raposo do Amaral ◽  
Cibele Biondo

AbstractBased on microsatellite prospection, we isolated and characterized 21 microsatellite markers for the Sooty Swift (Cypseloides fumigatus) and tested the cross-amplification in the White-collared Swift (Streptoprocne zonaris). Both species are New World species included in the Apodidae family. From these 21, only 13 loci were polymorphic in the Sooty Swift, and their levels of polymorphism were surprisingly low compared to related species. Cross-amplification in the White-collared Swift was successful for 11 loci of the 13 polymorphic found for the Sooty Swift, but seven were monomorphic and four were biallelic. The microsatellites described here could be useful in future genetic population studies for Sooty Swifts and related species.


2008 ◽  
Vol 1 (1) ◽  
pp. 337-339
Author(s):  
R. Jacewicz ◽  
M. Jedrzejczyk ◽  
M. Ludwikowska ◽  
J. Berent

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 687-703 ◽  
Author(s):  
Tijana Banjanac ◽  
Marijana Skoric ◽  
Mario Belamaric ◽  
Jasmina Nestorovic-Zivkovic ◽  
Danijela Misic ◽  
...  

Available scientific literature reports very few microsatellite markers derived from tetraploid genomes using de novo transcriptome sequencing, mostly because their gain usually represents a major computational challenge due to complicated combinatorics during assembly of sequence reads. Here we present a novel approach for mining polymorphic microsatellite loci from transcriptome data in a tetraploid species with no reference genome available. Pairs of 114 bp long de novo sequenced transcriptome reads of Centaurium erythraea were merged into short contigs of 170-200 bp each. High accuracy assembly of the pairs of reads was accomplished by a minimum of 14 bp overlap. Sequential bioinformatics operations involved fully free and open-source software and were performed using an average personal computer. Out of the 13 150 candidate contigs harboring SSR motifs obtained in a final output, we randomly chose 16 putative markers for which we designed primers. We tested the effectiveness of the established bioinformatics approach by amplifying them in eight different taxa within the genus Centaurium having various ploidy levels (diploids, tetraploids and hexaploids). Nine markers displayed polymorphism and/or transferability among studied taxa. They provided 54 alleles in total, ranging from 2 to 14 alleles per locus. The highest number of alleles was observed in C. erythraea, C. littorale and a hybridogenic taxon C. pannonicum. The developed markers are qualified to be used in genetic population studies on declining natural populations of Centaurium species, thus providing valuable information to evolutionary and conservation biologists. The developed cost-effective methodology provides abundant de novo assembled short contigs and holds great promise to mine numerous additional EST-SSR-containing markers for possible use in genetics population studies of tetraploid taxa within the genus Centaurium.


2021 ◽  
Author(s):  
Vanessa Bernardes ◽  
Devanir M. Murakami ◽  
Nair Bizão ◽  
Tamara N. Souza ◽  
Marcos J. da Silva ◽  
...  

Abstract Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.


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