Sequence-based diversity of 23 autosomal STR loci in Koreans investigated using an in-house massively parallel sequencing panel

2017 ◽  
Vol 30 ◽  
pp. 134-140 ◽  
Author(s):  
Eun Hye Kim ◽  
Hwan Young Lee ◽  
So Yeun Kwon ◽  
Eun Young Lee ◽  
Woo Ick Yang ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Parallel Sequencing ◽  
Str Loci ◽  
Autosomal Str

scholarly journals High polymorphism detected by massively parallel sequencing of autosomal STRs using old blood samples from a Chinese Han population

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Wenshen Dai ◽  
Yajiao Pan ◽  
Xiaochen Sun ◽  
Riga Wu ◽  
Luo Li ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Chinese Han Population ◽  
Massively Parallel ◽  
Library Preparation ◽  
Chinese Han ◽  
Discrimination Power ◽  
Blood Samples ◽  
Parallel Sequencing ◽  
Han Population ◽  
Str Loci

AbstractThe development of massively parallel sequencing (MPS) has quickly changed forensic short tandem repeat (STR) genotyping. By providing detailed sequence information, MPS technology may be used as an alternative or additional method to overcome the limitations of capillary electrophoresis-based STR profiling. Most current NGS processes are labour-intensive with regard to library preparation and require high-quality DNA template. In this study, a 16-plex STR typing system (SeqType®R16) was used to achieve direct library preparation without DNA extraction and adaptor ligation. The efficiency of this system was tested in 601 individuals, including 593 old blood samples from the Chinese Han population and eight positive controls. It took approximately 4 hours for library preparation, including blood direct multiplex PCR (1.5 hours), mixing of the product (15 minutes), single tube purification (2 hours) and quantification (15 minutes). The results showed that MPS presented a broader allele range and higher discrimination power. Except for FGA and D19S433, the allele number almost doubled or more than doubled at all complex STR loci and simple STR loci, including D13S317, D16S539, D5S818, and D7S820. The range of discrimination power increased from 0.8008–0.9572 to 0.8401–0.9753, and the culminated matching probability decreased from 1.7 × 10−15 to 1.1 × 10−17.

Estimating number of contributors in massively parallel sequencing data of STR loci

2019 ◽  
Vol 38 ◽  
pp. 15-22 ◽  
Author(s):  
Brian A Young ◽  
Katherine Butler Gettings ◽  
Bruce McCord ◽  
Peter M. Vallone
Keyword(s):  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Sequencing Data ◽  
Parallel Sequencing ◽  
Str Loci

Investigation into the sequence structure of 23 Y chromosomal STR loci using massively parallel sequencing

2016 ◽  
Vol 25 ◽  
pp. 132-141 ◽  
Author(s):  
So Yeun Kwon ◽  
Hwan Young Lee ◽  
Eun Hye Kim ◽  
Eun Young Lee ◽  
Kyoung-Jin Shin
Keyword(s):  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Sequence Structure ◽  
Parallel Sequencing ◽  
Str Loci

The First Autosomal STR Population Data of Kinh Ethinic Group in Vietnam by Using Massively Parallel Sequencing

2021 ◽  
Vol 57 (8) ◽  
pp. 985-988
Author(s):  
N. S. Pham ◽  
H. L. Tran ◽  
T. H. T. Nguyen ◽  
V. H. Nguyen ◽  
H. Hoang ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Population Data ◽  
Massively Parallel ◽  
Parallel Sequencing ◽  
Autosomal Str

Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems

2019 ◽  
Vol 133 (5) ◽  
pp. 1369-1380 ◽  
Author(s):  
Elena I. Zavala ◽  
Swetha Rajagopal ◽  
George H. Perry ◽  
Ivana Kruzic ◽  
Željana Bašić ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Massively Parallel Sequencing ◽  
Dna Typing ◽  
Dna Degradation ◽  
Massively Parallel ◽  
Parallel Sequencing ◽  
Autosomal Str
Sign in / Sign up

Export Citation Format

Share Document