Isolation and characterization of a plasmid DNA from periodontopathogenic bacterium, Eikenella corrodens 1073, which affects pilus formation and colony morphology

Gene ◽  
2005 ◽  
Vol 351 ◽  
pp. 143-148 ◽  
Author(s):  
Hiroyuki Azakami ◽  
Hiromi Akimichi ◽  
Masakatsu Usui ◽  
Hiromichi Yumoto ◽  
Shigeyuki Ebisu ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Colony Morphology ◽  
Eikenella Corrodens ◽  
Isolation And Characterization

Isolation and Plasmid Characterization of a Lactobacillus Species Involved in the Manufacture of Fermented Sausage1

1985 ◽  
Vol 48 (12) ◽  
pp. 1028-1035 ◽  
Author(s):  
DENNIS A. ROMERO ◽  
LARRY L. McKAY
Keyword(s):  
Lactic Acid ◽  
Polyethylene Glycol ◽  
Plasmid Dna ◽  
Acid Production ◽  
Gas Production ◽  
Lactobacillus Species ◽  
Erythromycin Resistance ◽  
Streptococcus Lactis ◽  
Isolation And Characterization

Isolation and characterization of a Lactobacillus species capable of proper acid production in a sausage environment is described. The isolate from sausage, categorized as a lactobacillus in the subgenus Streptobacterium, was designated Lactobacillus sp. DR1. Growth occurred at 5 and 42°C but not at 45°C. Fructose, galactose, glucose, mannose, melibiose, N-acetylglucosamine, ribose, sucrose and trehalose were fermented. Gas production from glucose was not observed. In MRS glucose broth, D(−) and L(+) lactic acid were produced. Lactobacillus sp. DR1 contained a single cryptic plasmid of approximately 30 megadaltons (Mdal). In sausage fermentation trials, both Lactobacillus sp. DR1 and plasmid-free derivative DR1C lowered the pH to below 5.3 after 8 h in the smokehouse. Conjugation was demonstrated through the transfer of plasmid pAMβ1, which encodes erythromycin resistance, from Streptococcus lactis 2301β to Lactobacillus sp. DR1. Mutanolysin-generated protoplasts could be regenerated using 0.5 M ammonium chloride, lactose, maltose or sucrose as osmotic stabilizers. Regeneration frequencies ranged from less than 1.0% up to 35%; however, transformation of Lactobacillus sp. DR1 protoplasts by plasmid DNA in the presence of polyethylene glycol (PEG) was unsuccessful.

scholarly journals Isolation and Characterization of Plasmid DNA in Lactobacillus acidophilus.

1991 ◽  
Vol 55 (8) ◽  
pp. 2051-2056 ◽  
Author(s):  
Kazuo KANATANI ◽  
Kazushi YOSHIDA ◽  
Takatsugu TAHARA ◽  
Hirosumi MIURA ◽  
Masaru SAKAMOTO ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Lactobacillus Acidophilus ◽  
Isolation And Characterization

Isolation and characterization of plasmid DNA fromRuminococcus

1987 ◽  
Vol 16 (2) ◽  
pp. 97-100 ◽  
Author(s):  
Roderick V. Asmundson ◽  
William J. Kelly
Keyword(s):  
Plasmid Dna ◽  
Isolation And Characterization

Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples

2019 ◽  
pp. 3-20
Author(s):  
Kaitlin A. Tagg ◽  
Carola Venturini ◽  
Muhammad Kamruzzaman ◽  
Andrew N. Ginn ◽  
Sally R. Partridge
Keyword(s):  
Plasmid Dna ◽  
Dna Isolation ◽  
Clinical Samples ◽  
Isolation And Characterization

Isolation and Characterization of Plasmid DNA inLactobacillus acidophilus

1991 ◽  
Vol 55 (8) ◽  
pp. 2051-2156
Author(s):  
Kazuo Kanatani ◽  
Kazushi Yoshida ◽  
Takatsugu Tahara ◽  
Hirosumi Miura ◽  
Masaru Sakamoto ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of plasmid DNA from Bacteroides melaninogenicus subspecies melaninogenicus.

1983 ◽  
Vol 25 (4) ◽  
pp. 1158-1161 ◽  
Author(s):  
Yoshimitsu Abiko ◽  
Hideo Onose ◽  
Shigeno Saito ◽  
Hisashi Takiguchi
Keyword(s):  
Plasmid Dna ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Rugose Form of Vibrio cholerae O139 Strain MO10

1999 ◽  
Vol 67 (2) ◽  
pp. 958-963 ◽  
Author(s):  
Yoshimitsu Mizunoe ◽  
Sun Nyunt Wai ◽  
Akemi Takade ◽  
Shin-Ichi Yoshida
Keyword(s):  
Cell Surface ◽  
Vibrio Cholerae ◽  
Microscopic Examination ◽  
Biofilm Formation ◽  
Nutrient Starvation ◽  
Colony Morphology ◽  
Isolation And Characterization ◽  
Slime Layer ◽  
Vibrio Cholerae O139

ABSTRACT An extracellular exopolysaccharide (slime) is produced byVibrio cholerae O139 MO10 in response to nutrient starvation. The presence of this slime layer on the cell surface and its subsequent release have been shown to be associated with biofilm formation and the change from a normal smooth colony morphology to a rugose one. An immunoelectron microscopic examination demonstrated that there is an epitope common to the exopolysaccharide antigen of V. cholerae O1 and that of O139 MO10.

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