Robust reference gene design and validation for expression studies in the large milkweed bug, Oncopeltus fasciatus, upon cardiac glycoside stress

Gene ◽  
2019 ◽  
Vol 710 ◽  
pp. 66-75 ◽  
Author(s):  
Fee Meinzer ◽  
Susanne Dobler ◽  
Alexander Donath ◽  
Jennifer N. Lohr
Keyword(s):  
Reference Gene ◽  
Cardiac Glycoside ◽  
Oncopeltus Fasciatus ◽  
Gene Design ◽  
Milkweed Bug ◽  
Expression Studies

Studies on the pteridines in the milkweed bug, Oncopeltus fasciatus (Dallas)

1966 ◽  
Vol 12 (11) ◽  
pp. 1411-1421 ◽  
Author(s):  
Hugh S. Forrest ◽  
Michael Menaker ◽  
Jennifer Alexander
Keyword(s):  
Oncopeltus Fasciatus ◽  
Milkweed Bug

scholarly journals Selection and Validation of Appropriate Reference Genes for Gene Expression Studies in Schima Superba

2021 ◽  
Author(s):  
Zhongyi Yang ◽  
Rui Zhang ◽  
Zhichun Zhou
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Reference Genes ◽  
Geometric Mean ◽  
Gene Transcript ◽  
Expression Stability ◽  
Schima Superba ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Different Tissues

Abstract Background Quantitative real-time PCR (qRT-PCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. Schima superba is a strong resistance and fast-growing timber specie. However, so far, reliable reference gene identifications have not been reported in S. superba. In this study, we screened and verified the stably expressed reference genes in different tissues of S. superba.Results Nineteen candidate reference genes were selected and evaluated for their expression stability in different tissues. Three software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate the reference gene transcript stabilities, and comprehensive stability ranking was generated by the geometric mean method. Our results identified that SsuACT was the most stable reference gene, SsuACT + SsuRIB was the best reference genes combination for different tissues. Finally, the stable and less stable reference genes were verified using the SsuSND1 expression in different tissues.Conclusions This is the first report to verify the appropriate reference genes for normalizing gene expression in S. superba for different tissues, which will facilitate future elucidation of gene regulations in this species, and useful references for relative species.

scholarly journals Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108746 ◽  
Author(s):  
Felipe de Almeida Dias ◽  
Luiz Ricardo da Costa Vasconcellos ◽  
Alexandre Romeiro ◽  
Marcia Attias ◽  
Thais Cristina Souto-Padrón ◽  
...  
Keyword(s):  
Oncopeltus Fasciatus ◽  
Milkweed Bug

The Hormonal Control of the Development of Hairs and Bristles in the Milkweed Bug, ONCOPELTUS FASCIATUS, DALL

1966 ◽  
Vol 44 (3) ◽  
pp. 507-522
Author(s):  
PETER A. LAWRENCE
Keyword(s):  
Juvenile Hormone ◽  
Mode Of Action ◽  
Hormonal Control ◽  
Oncopeltus Fasciatus ◽  
Corpora Allata ◽  
Moult Cycle ◽  
Dense Population ◽  
Milkweed Bug ◽  
Larval Stages ◽  
Hormonal Milieu

1. As in Rhodnius, the larval Oncopeltus has bristles which are supplemented at each moult. However, at metamorphosis a dense population of non-innervated hairs develops. 2. Implantation of corpora allata into 5th-stage larvae showed that the development of these hairs can be inhibited universally or locally by the juvenile hormone (JH). 3. Transplantations of integument between 5th-stage larvae of different stages in the moult cycle gave some information about the power of the host to synchronize the graft to its own moult cycle. 4. Transplantations between different larval stages showed that the grafted in tegument responded to the hormonal milieu of the host. 5. Adult integument was transplanted onto larvae to study the reversal of metamorphosis. It was found that the development of a supernumerary population of hairs depended on the integument passing through a moult cycle in the presence of JH. After two moults in the presence of JH, reversal of metamorphosis was found to vary over the surface of the transplant, being further advanced at the margin. At the edge of the graft properly formed larval bristles developed, while at the centre adult hairs were formed in adult cuticle. Intermediately formed bristles were found in the intervening areas. It is suggested that reactions associated with wounding are the cause of this heterogeneous result. 6. The significance of these results in relation to other work and to theories concerning the mode of action of the juvenile hormone is discussed.

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