Mo1369 Fluorescence in Situ Hybridization (FISH) for Endoscopic Diagnosis of Pancreatobiliary Malignancy: a Systematic Review and Meta-Analysis

2015 ◽  
Vol 81 (5) ◽  
pp. AB396
Author(s):  
Tarun Rustagi ◽  
Thomas Mccarty
Keyword(s):  
Systematic Review ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Meta Analysis ◽  
Endoscopic Diagnosis

scholarly journals Concordance Rate between HER2 Immunohistochemistry and in Situ Hybridization in Gastric Carcinoma: Systematic Review and Meta-Analysis

2016 ◽  
Vol 31 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Jung-Soo Pyo ◽  
Jin Hee Sohn ◽  
Woo Ho Kim
Keyword(s):  
Systematic Review ◽  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Diagnostic Accuracy ◽  
Diagnostic Test ◽  
Meta Analysis ◽  
Diagnostic Test Accuracy ◽  
Test Accuracy ◽  
Significant Difference

Purpose The aim of this study was to investigate the diagnostic accuracy of HER2 immunohistochemistry (IHC) in gastric carcinoma (GC) through a systematic review, meta-analysis and diagnostic test accuracy review. Method The current study included 12,679 GC cases and 181 subsets in 45 eligible studies. We performed concordance analysis between HER2 IHC and in situ hybridization (ISH) in GC. Diagnostic test accuracy was analyzed and the area under the curve (AUC) on the summary receiver operating characteristic (SROC) curve was calculated. Results HER2 amplification rates were 3.0%, 31.8%, and 93.0% in the IHC score 0/1+, 2+, and 3+ groups, respectively. The concordance rates between IHC and ISH were 0.969 (95% confidence interval [CI] 0.962-0.975), 0.393 (95% CI 0.331-0.458) and 0.915 (95% CI 0.882-0.939) in the HER2 IHC score 0/1+, 2+, and 3+ groups, respectively. For all the HER2 IHC score groups, the positive rates were higher in the silver ISH (SISH) subgroup than in the fluorescence ISH (FISH) and chromogenic ISH (CISH) subgroups. In diagnostic test accuracy review, the pooled sensitivity and specificity were 0.86 (95% CI 0.84-0.87) and 0.91 (95% CI 0.90-0.91). The AUC on SROC curve was 0.958. However, there was no significant difference in the values of AUC between the ISH methods. Conclusions Our results showed that HER2 IHC was well concordant with ISH in HER2 IHC score 0/1+ or 3+. Although this meta-analysis showed higher diagnostic accuracy of HER2 IHC, more detailed criteria for HER2 IHC score 2+ cases will be required to predict HER2 status.

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