More effective removal of antibiotic resistance genes from excess sludge by microwave integrated fenton treatment

2020 ◽  
Vol 149 ◽  
pp. 104920
Author(s):  
Guolan Wang ◽  
Dahang Deng ◽  
Chunyan Hu ◽  
Liping Lou ◽  
Ling Luo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Excess Sludge ◽  
Effective Removal

scholarly journals Combined membrane filtration and 265 nm UV irradiation for effective removal of cell free antibiotic resistance genes from feed water and concentrate

2020 ◽  
Vol 598 ◽  
pp. 117676 ◽  
Author(s):  
Pawel Krzeminski ◽  
Edward Feys ◽  
Marc Anglès d'Auriac ◽  
Aina Charlotte Wennberg ◽  
Muhammad Umar ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Uv Irradiation ◽  
Resistance Genes ◽  
Membrane Filtration ◽  
Antibiotic Resistance Genes ◽  
Feed Water ◽  
Effective Removal

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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