Characterization of an indigenous quorum quenching bacterium and its effect on the SMP, EPS, and microbial community of sludge mixture during wastewater treatment

2020 ◽  
Vol 152 ◽  
pp. 104995 ◽  
Author(s):  
Xinjiao Dong ◽  
Rui Zhu ◽  
Yaru Li ◽  
Jing Qin ◽  
Shimei Ge
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Quorum Quenching

Effects of Quorum Quenching on the Microbial Community of Biofilm in an Anoxic/Oxic MBR for Wastewater Treatment

2016 ◽  
Vol 26 (9) ◽  
pp. 1593-1604 ◽  
Author(s):  
Sung Jun Jo ◽  
Hyeokpil Kwon ◽  
So-Yeon Jeong ◽  
Sang Hyun Lee ◽  
Hyun-Suk Oh ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Quorum Quenching

Prediction of the O2 Conditions in the Selector

1994 ◽  
Vol 29 (7) ◽  
pp. 229-237 ◽  
Author(s):  
J. Kruit ◽  
F. Boley ◽  
L. J. A. M. Jacobs ◽  
T. W. M. Wouda
Keyword(s):  
Wastewater Treatment ◽  
Pilot Plant ◽  
Wastewater Treatment Plants ◽  
Coarse Filter ◽  
Plant Research ◽  
Influent Characterization

Influent characterization and biosorption experiments were carried out with settled influent of seven wastewater treatment plants to study the influence of O2 in the selector in relation to the success of developing good settling properties of the sludge. In previous years working selectors were installed and/or pilot plant research was carried out at these wastewater treatment plants. Characterization of the influent was done with help of standard COD and BOD measurements with help of a coarse filter. The research has elucidated that the presence of O2 in the selector, at initial sludge loadings of 3.5-6.5 kg BOD/kg MLSS.d, is important for producing good settling properties of the sludge when the sum of readily biodegradable COD and rapidly hydrolysable COD is greater than 40%. When the sum of sludge COD and slow hydrolysable COD is greater than 50% an unaerated selector can be used.

scholarly journals Pollutant-Removing Biofilter Strains Associated with High Ammonia and Hydrogen Sulfide Removal Rate in a Livestock Wastewater Treatment Facility

2021 ◽  
Vol 13 (13) ◽  
pp. 7358
Author(s):  
Dong-Hyun Kim ◽  
Hyun-Sik Yun ◽  
Young-Saeng Kim ◽  
Jong-Guk Kim
Keyword(s):  
Wastewater Treatment ◽  
Hydrogen Sulfide ◽  
Microbial Community ◽  
Microbial Communities ◽  
Illumina Miseq ◽  
Illumina Miseq Sequencing ◽  
Miseq Sequencing ◽  
Treatment Facility ◽  
Livestock Wastewater ◽  
Wastewater Treatment Facility

This study analyzed the microbial community metagenomically to determine the cause of the functionality of a livestock wastewater treatment facility that can effectively remove pollutants, such as ammonia and hydrogen sulfide. Illumina MiSeq sequencing was used in analyzing the composition and structure of the microbial community, and the 16S rRNA gene was used. Through Illumina MiSeq sequencing, information such as diversity indicators as well as the composition and structure of microbial communities present in the livestock wastewater treatment facility were obtained, and differences between microbial communities present in the investigated samples were compared. The number of reads, operational taxonomic units, and species richness were lower in influent sample (NLF), where the wastewater enters, than in effluent sample (NL), in which treated wastewater is found. This difference was greater in June 2019 than in January 2020, and the removal rates of ammonia (86.93%) and hydrogen sulfide (99.72%) were also higher in June 2019. In both areas, the community composition was similar in January 2020, whereas the influent sample (NLF) and effluent sample (NL) areas in June 2019 were dominated by Proteobacteria (76.23%) and Firmicutes (67.13%), respectively. Oleiphilaceae (40.89%) and Thioalkalibacteraceae (12.91%), which are related to ammonia and hydrogen sulfide removal, respectively, were identified in influent sample (NLF) in June 2019. They were more abundant in June 2019 than in January 2020. Therefore, the functionality of the livestock wastewater treatment facility was affected by characteristics, including the composition of the microbial community. Compared to Illumina MiSeq sequencing, fewer species were isolated and identified in both areas using culture-based methods, suggesting Illumina MiSeq sequencing as a powerful tool to determine the relevance of microbial communities for pollutant removal.

scholarly journals Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei
Keyword(s):  
Wastewater Treatment ◽  
Xylanase Activity ◽  
Pulp And Paper ◽  
Optimal Conditions ◽  
Paper Wastewater ◽  
Termite Hindgut ◽  
Uncultured Microorganisms ◽  
Metagenomic Approach ◽  
Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

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