Performance and characterization of the microbial community structures in the activated sludge from wastewater treatment plant at high altitudes in Tibet of China

2018 ◽  
Vol 106 ◽  
pp. 108-115 ◽  
Author(s):  
Jin Xu ◽  
Peifang Wang ◽  
Yi Li ◽  
Lihua Niu
2021 ◽  
Vol 4 ◽  
Author(s):  
Angelantonio Calabrese ◽  
Erika Loi ◽  
Laura Mandrelli ◽  
Massimo Blonda

The growing biomass in the wastewater treatment plant is called "activated sludge"; usually it is mainly composed by bacteria (95%) and by Protozoa and Metazoans (5%). The sludge biological composition is a good indicator of the wastewater treatment plant state of health. In this study, an initial characterization of the microorganisms present in the activated sludge is carried out through DNA analysis.The aim of this activity is the development of the procedures for the characterization of the sludge of the biological oxidation lines, to identify microorganism throught a new approach using biomolecular analysis and the definition of new standard protocols.


2011 ◽  
Vol 4 (5) ◽  
pp. 628-642 ◽  
Author(s):  
Olga Sánchez ◽  
Laura Garrido ◽  
Irene Forn ◽  
Ramon Massana ◽  
Manuel Ignacio Maldonado ◽  
...  

2008 ◽  
Vol 57 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Pamela Principi ◽  
Federica Villa ◽  
Barbara Giussani ◽  
Elisabetta Zanardini ◽  
Francesca Cappitelli ◽  
...  

2012 ◽  
Vol 66 (10) ◽  
pp. 2155-2161 ◽  
Author(s):  
Purnika Damindi Ranasinghe ◽  
Hiroyasu Satoh ◽  
Mamoru Oshiki ◽  
Kenshiro Oshima ◽  
Wataru Suda ◽  
...  

The diversity of bacterial groups in activated sludge from large- and small-scale wastewater treatment plants was explored by barcoded pyrosequencing of 16S rRNA gene. Activated sludge samples (three small and 17 large scale) were collected from 12 wastewater treatment plants to clarify precise taxonomy and relative abundances. DNA was extracted, and amplified by 4 base barcoded 27f/519r primer set. The 454 Titanium (Roche) pyrosequences were obtained and analyses performed by Quantitative Insight Into Microbial Ecology (QIIME) with around 100,000 reads. Sequence statistics were computed, while constructing a phylogenetic tree and heatmap. Computed results explained total microbial diversity at phylum and class level and resolution was further extended to Operational Taxonomic Unit (OTU) based taxonomic assignment for investigating community distribution based on individual sample. Composition of sequence reads were compared and microbial community structures for large- and small-scale treatment plants were identified as major phyla (Proteobacteria and Bacteroidetes) and classes (Betaproteobacteria and Bacteroidetes). Also, family level breakdowns were explained and differences in family Nitrospiraceae and phylum Actinobacteria found at their species level were also illustrated. Thus, the pyrosequencing method provides high resolution insight into microbial community structures in activated sludge that might have been unnoticed with conventional approaches.


1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.


1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.


1997 ◽  
Vol 36 (2-3) ◽  
pp. 1-8 ◽  
Author(s):  
P. Grau ◽  
B. P. Da-Rin

An unusually severe case of toxicity accompanied by activated sludge filamentous bulking was observed at the wastewater treatment plant Sao Paulo-Barueri. Treatment efficiency of the plant, operated without major problems for more than five years before, was significantly hindered for almost six months. Occurrence of toxic shocks was confirmed partly directly but mostly indirectly by inhibition of nitrification and biological phenomena related to toxicity. Several measures adopted, including the recycled activated sludge chlorination, are described in the paper.


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