Immune-responsive lysozymes from hemocytes of the American dog tick, Dermacentor variabilis and an embryonic cell line of the Rocky Mountain wood tick, D. andersoni

2004 ◽  
Vol 34 (12) ◽  
pp. 1235-1246 ◽  
Author(s):  
Jason A. Simser ◽  
Kevin R. Macaluso ◽  
Albert Mulenga ◽  
Abdu F. Azad
Keyword(s):  
Cell Line ◽  
Rocky Mountain ◽  
Dermacentor Variabilis ◽  
Embryonic Cell ◽  
American Dog Tick ◽  
Embryonic Cell Line ◽  
Dog Tick ◽  
Rocky Mountain Wood Tick

scholarly journals Isolation of a Spotted Fever Group Rickettsia, Rickettsia peacockii, in a Rocky Mountain Wood Tick, Dermacentor andersoni, Cell Line

2001 ◽  
Vol 67 (2) ◽  
pp. 546-552 ◽  
Author(s):  
Jason A. Simser ◽  
Ann T. Palmer ◽  
Ulrike G. Munderloh ◽  
Timothy J. Kurtti
Keyword(s):  
Cell Line ◽  
Citrate Synthase ◽  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Rocky Mountain ◽  
Embryonic Cell ◽  
Host Cells ◽  
Spotted Fever Group ◽  
Dermacentor Andersoni ◽  
Rocky Mountain Wood Tick

ABSTRACT An embryonic cell line (DAE100) of the Rocky Mountain wood tick,Dermacentor andersoni, was observed by microscopy to be chronically infected with a rickettsialike organism. The organism was identified as a spotted fever group (SFG) rickettsia by PCR amplification and sequencing of portions of the 16S rRNA, citrate synthase, Rickettsia genus-specific 17-kDa antigen, and SFG-specific 190-kDa outer membrane protein A (rOmpA) genes. Sequence analysis of a partial rompA gene PCR fragment and indirect fluorescent antibody data for rOmpA and rOmpB indicated that this rickettsia was a strain (DaE100R) of Rickettsia peacockii, an SFG species presumed to be avirulent for both ticks and mammals.R. peacockii was successfully maintained in a continuous culture of DAE100 cells without apparent adverse effects on the host cells. Establishing cell lines from embryonic tissues of ticks offers an alternative technique for isolation of rickettsiae that are transovarially transmitted.

Experiments on Oral Secretion of the Rocky Mountain Wood Tick, Dermacentor andersoni Stiles (Acarina: Ixodidae)

1957 ◽  
Vol 89 (1) ◽  
pp. 1-5 ◽  
Author(s):  
J. D. Gregson
Keyword(s):  
British Columbia ◽  
Pacific Northwest ◽  
Rocky Mountain ◽  
Dermacentor Variabilis ◽  
Oral Secretion ◽  
Amblyomma Maculatum ◽  
Dermacentor Andersoni ◽  
The Pacific ◽  
Tick Paralysis ◽  
Rocky Mountain Wood Tick

Tick paralysis continues to be one of the most baffling and fascinating tickborne diseases in Canada. It was first reported in this country by Todd in 1912. Since then about 250 human cases, including 28 deaths, have been recorded from British Columbia. Outbreaks in cattle have affected up to 400 animals at a time, with losses in a herd as high as 65 head. Although the disease is most common in the Pacific northwest, where it is caused by the Rocky Mountain wood tick, Dermacentor andersoni Stiles, it has lately been reported as far south as Florida and has been produced by Dermacentor variabilis Say, Amblyomma maculatum Koch, and A. americanum (L.) (Gregson, 1953). The symptoms include a gradual ascending symmetrical flaccid paralysis. Apparently only man, sheep, cattle, dogs, and buffalo (one known instance) are susceptible, but even these may not necessarily be paralysed.

Synganglion transcriptome and developmental global gene expression in adult females of the American dog tick, Dermacentor variabilis (Acari: Ixodidae)

2011 ◽  
Vol 20 (4) ◽  
pp. 465-491 ◽  
Author(s):  
B. W. Bissinger ◽  
K. V. Donohue ◽  
S. M. S. Khalil ◽  
C. M. Grozinger ◽  
D. E. Sonenshine ◽  
...  
Keyword(s):  
Gene Expression ◽  
Global Gene Expression ◽  
Dermacentor Variabilis ◽  
American Dog Tick ◽  
Adult Females ◽  
Dog Tick
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