Changes in the activity, dissociation, aggregation, and the secondary and tertiary structures of a thaumatin-like protein with a high polyphenol oxidase activity induced by high pressure CO2

2014 ◽  
Vol 23 ◽  
pp. 68-78 ◽  
Author(s):  
Renjie Li ◽  
Yongtao Wang ◽  
Wanfeng Hu ◽  
Xiaojun Liao
Keyword(s):  
High Pressure ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Polyphenol Oxidase Activity ◽  
Tertiary Structures ◽  
High Pressure Co2

Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw (Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage

2019 ◽  
Vol 25 (8) ◽  
pp. 711-722
Author(s):  
Robert G Brannan ◽  
Ahmed Faik ◽  
Ryan Goelz ◽  
Sivakumar Pattathil
Keyword(s):  
High Pressure ◽  
Cell Wall ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Cell Walls ◽  
High Pressure Processing ◽  
Cell Wall Composition ◽  
Refrigerated Storage ◽  
Polyphenol Oxidase Activity ◽  
Asimina Triloba

This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw ( Asimina triloba) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage. We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning. Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition. Results show that both varieties have typical type I primary cell walls of flowering dicots. However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls. High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.

Impact of high pressure processing on color, bioactive compounds, polyphenol oxidase activity, and microbiological attributes of pumpkin purée

2015 ◽  
Vol 22 (3) ◽  
pp. 235-245 ◽  
Author(s):  
Francisco González-Cebrino ◽  
Rocío Durán ◽  
Jonathan Delgado-Adámez ◽  
Rebeca Contador ◽  
Rosario Ramírez Bernabé
Keyword(s):  
High Pressure ◽  
Polyphenol Oxidase ◽  
Bioactive Compounds ◽  
Oxidase Activity ◽  
High Pressure Processing ◽  
Polyphenol Oxidase Activity

Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels

1997 ◽  
Vol 34 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Antonio Rescigno ◽  
Francesca Sollai ◽  
Andrea C Rinaldi ◽  
Giulia Soddu ◽  
Enrico Sanjust
Keyword(s):  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Polyphenol Oxidase Activity ◽  
Activity Staining ◽  
Polyacrylamide Electrophoresis
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