Stem cell-based dental tissue regeneration is a new and exciting field that has the potential to transform the way that we practice dentistry. It is, however, imperative its clinical application is supported by solid basic and translational research. In this way, the full extent of the potential risks involved in the use of these technologies will be understood, and the means to prevent them will be discovered. Therefore, the aim of this chapter is to analyze the state-of-the-science with regard to dental pulp stem cell research in dental tissue engineering, the new developments in biomimetic scaffold materials customized for dental tissue applications, and to give a prospectus with respect to translational approaches of these research findings towards clinical application.