Suitable solutions for reconstituting the ultrasound contrast agent “Levovist” used in contrast echocardiogram: In vitro and in vivo evaluation of the influence of osmotic pressure

2009 ◽  
Vol 136 (3) ◽  
pp. 335-340 ◽  
Author(s):  
Yumi Shiina ◽  
Nobusada Funabashi ◽  
Kiyomi Teramoto ◽  
Masae Uehara ◽  
Hiroyuki Takaoka ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Osmotic Pressure ◽  
Ultrasound Contrast Agent ◽  
In Vivo Evaluation ◽  
Ultrasound Contrast

scholarly journals Accelerated sonothrombolysis with Definity in a xenographic porcine cerebral thromboembolism model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Robert T. Kleven ◽  
Kunal B. Karani ◽  
Nicole Hilvert ◽  
Samantha M. Ford ◽  
Karla P. Mercado-Shekhar ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Combined Treatment ◽  
Ultrasound Contrast Agent ◽  
Vascular Occlusion ◽  
Recombinant Tissue Plasminogen Activator ◽  
Pulsed Ultrasound ◽  
Side Hole ◽  
Ultrasound Contrast

AbstractAdjuvant ultrasound at 2 MHz with or without an ultrasound contrast agent improves the rate of thrombus resolution by recombinant tissue plasminogen activator (rt-PA) in laboratory and clinical studies. A sub-megahertz approach can further expand this therapy to a subset of patients with an insufficient temporal bone window, improving efficacy in unselected patient populations. The aim of this study was to determine if a clinical ultrasound contrast agent (UCA), Definity, and 220 kHz pulsed ultrasound accelerated rt-PA thrombolysis in a preclinical animal model of vascular occlusion. The effect of Definity and ultrasound on thrombus clearance was first investigated in vitro and subsequently tested in a xenographic porcine cerebral thromboembolism model in vivo. Two different microcatheter designs (end-hole, multi-side-hole) were used to infuse rt-PA and Definity at the proximal edge or directly into clots, respectively. Sonothrombolysis with Definity increased clot mass loss relative to saline or rt-PA alone in vitro, only when rt-PA was administered directly into clots via a multi-side-hole microcatheter. Combined treatment with rt-PA, Definity, and ultrasound in vivo increased the rate of reperfusion up to 45 min faster than clots treated with rt-PA or saline. In this porcine cerebral thromboembolism model employing retracted human clots, 220 kHz ultrasound, in conjunction with Definity increased the probability of early successful reperfusion with rt-PA.

Using a Commercial Ultrasound Contrast Agent for Viral-Mediated Gene Transfer In Vitro and In Vivo

2007 ◽  
Author(s):  
Candace M. Howard ◽  
Flemming Forsberg ◽  
Ji-Bin Liu ◽  
Daniel A. Merton ◽  
Corrado Minimo ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast
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