Ultrasound contrast agent, Levovist microbubbles are phagocytosed by Kupffer cells—In vitro and in vivo studies

2006 ◽  
Vol 35 (4) ◽  
pp. 235-237 ◽  
Author(s):  
H IIJIMA ◽  
F MORIYASU ◽  
T MIYAHARA ◽  
K YANAGISAWA
Keyword(s):  
Contrast Agent ◽  
Kupffer Cells ◽  
Ultrasound Contrast Agent ◽  
In Vivo Studies ◽  
Ultrasound Contrast

scholarly journals Accelerated sonothrombolysis with Definity in a xenographic porcine cerebral thromboembolism model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Robert T. Kleven ◽  
Kunal B. Karani ◽  
Nicole Hilvert ◽  
Samantha M. Ford ◽  
Karla P. Mercado-Shekhar ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Combined Treatment ◽  
Ultrasound Contrast Agent ◽  
Vascular Occlusion ◽  
Recombinant Tissue Plasminogen Activator ◽  
Pulsed Ultrasound ◽  
Side Hole ◽  
Ultrasound Contrast

AbstractAdjuvant ultrasound at 2 MHz with or without an ultrasound contrast agent improves the rate of thrombus resolution by recombinant tissue plasminogen activator (rt-PA) in laboratory and clinical studies. A sub-megahertz approach can further expand this therapy to a subset of patients with an insufficient temporal bone window, improving efficacy in unselected patient populations. The aim of this study was to determine if a clinical ultrasound contrast agent (UCA), Definity, and 220 kHz pulsed ultrasound accelerated rt-PA thrombolysis in a preclinical animal model of vascular occlusion. The effect of Definity and ultrasound on thrombus clearance was first investigated in vitro and subsequently tested in a xenographic porcine cerebral thromboembolism model in vivo. Two different microcatheter designs (end-hole, multi-side-hole) were used to infuse rt-PA and Definity at the proximal edge or directly into clots, respectively. Sonothrombolysis with Definity increased clot mass loss relative to saline or rt-PA alone in vitro, only when rt-PA was administered directly into clots via a multi-side-hole microcatheter. Combined treatment with rt-PA, Definity, and ultrasound in vivo increased the rate of reperfusion up to 45 min faster than clots treated with rt-PA or saline. In this porcine cerebral thromboembolism model employing retracted human clots, 220 kHz ultrasound, in conjunction with Definity increased the probability of early successful reperfusion with rt-PA.

Using a Commercial Ultrasound Contrast Agent for Viral-Mediated Gene Transfer In Vitro and In Vivo

2007 ◽  
Author(s):  
Candace M. Howard ◽  
Flemming Forsberg ◽  
Ji-Bin Liu ◽  
Daniel A. Merton ◽  
Corrado Minimo ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast

Novel Difolate Targeting Nano-Level Ultrasound Contrast Agent for Therapy of Breast Cancer Tumor Cells

2021 ◽  
Vol 13 (7) ◽  
pp. 1295-1303
Author(s):  
Guangheng Liu ◽  
Xiangfeng Yang ◽  
Qiming Niu ◽  
Wenkui Sun
Keyword(s):  
Breast Cancer ◽  
Contrast Agent ◽  
Tumor Cells ◽  
Folate Receptor ◽  
Ultrasound Contrast Agent ◽  
Hydroxyl Group ◽  
Binding Ability ◽  
Ultrasound Contrast ◽  
Mcf 7

ABSTRACTA new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS) and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized. The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane, surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.

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