Targeting a polyketide synthase gene for Aspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR

2007 ◽  
Vol 115 (3) ◽  
pp. 313-318 ◽  
Author(s):  
Ali Atoui ◽  
Florence Mathieu ◽  
Ahmed Lebrihi
Keyword(s):  
Real Time ◽  
Ochratoxin A ◽  
Real Time Pcr ◽  
Polyketide Synthase ◽  
Aspergillus Carbonarius ◽  
Polyketide Synthase Gene

Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius

2014 ◽  
Vol 179 ◽  
pp. 10-17 ◽  
Author(s):  
Antonia Gallo ◽  
Benjamin P. Knox ◽  
Kenneth S. Bruno ◽  
Michele Solfrizzo ◽  
Scott E. Baker ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Aspergillus Carbonarius ◽  
Identification And Characterization

A comparative study of DNA extraction methods to be used in real-time PCR based quantification of ochratoxin A-producing molds in food products

Food Control ◽  
2012 ◽  
Vol 25 (2) ◽  
pp. 666-672 ◽  
Author(s):  
Alicia Rodríguez ◽  
Mar Rodríguez ◽  
M. Isabel Luque ◽  
Annemarie F. Justesen ◽  
Juan J. Córdoba
Keyword(s):  
Comparative Study ◽  
Real Time ◽  
Ochratoxin A ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Food Products ◽  
Extraction Methods ◽  
Dna Extraction Methods

scholarly journals Detection of ochratoxin A by aptamer-assisted real-time PCR-based assay (Apta-qPCR)

2017 ◽  
Vol 17 (8) ◽  
pp. 923-930 ◽  
Author(s):  
Harshvardhan Modh ◽  
Thomas Scheper ◽  
Johanna-Gabriela Walter
Keyword(s):  
Real Time ◽  
Ochratoxin A ◽  
Real Time Pcr

scholarly journals DEVELOPMENT OF THE REAL-TIME PCR METHODOLOGY FOR TESTING MYCOTOXIGENIC MICROORGANISMS IN GRAPE MARC

2021 ◽  
Vol 28 (3) ◽  
pp. 187-194
Author(s):  
Rodica Sturza ◽  
◽  
Valentin Mitin ◽  
Irina Mitina ◽  
Dan Zgardan ◽  
...  
Keyword(s):  
Real Time ◽  
Ochratoxin A ◽  
Real Time Pcr ◽  
Modern Society ◽  
Fungal Species ◽  
Peptide Sequence ◽  
Wine Production ◽  
The Real ◽  
Grape Marc ◽  
Industrial Waste Management

Agro-industrial waste management is an important problem of modern society as agriculture and food industry are important sources of waste. Wine production generates a considerable amount of winemaking waste (grape marc). Grape marc can be a source of natural dyes, antioxidants and could have various applications, if it is confirmed that it does not contain technogenic contaminants or unwanted microorganisms, for example, producers of mycotoxins. The paper developed the Real -Time Polymerase Chain Reaction (Real-Time PCR) methodology for testing the presence of potentially mycotoxogenic fungal species capable of producing ochratoxin A (OTA), which could be applied before grape marc processing. Based on the non-ribosomal peptide sequence of OTA, involved in ochratoxin biosynthesis, the primers have been developed for the detection of microorganisms potentially capable of producing ochratoxin A.

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