Real Time Pcr Detection Of Aspergllus Fumigatus, Aspergillus Flavus And Aspergillus Niger Based On Polyketide Synthase Gene

In this study, a set of real-time PCR primers for the detection of aflatoxin in peanut was successfully designed according to the AFLR gene of Aspergillus niger. Upstream primer was 5'-AACCTGATGACGACTGATAT-3'; Downstream primer was 5'-AGCACCTTGAGAACGATAA-3'; The real-time PCR detection system established in this study had good specificity, and the sensitivity of DNA detection can reach 3×10-13 ng/ul. The correlation coefficient between the contents of aflatoxin in peanut samples and PCR Ct of Real-time was-0.718, and the correlation coefficient was significantly negative.

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Development of a Real Time PCR System for Detection of Penicillium nordicum and for Monitoring Ochratoxin A Production in Foods by Targeting the Ochratoxin Polyketide Synthase Gene

Systematic and Applied Microbiology ◽

10.1078/0723202041438419 ◽

2004 ◽

Vol 27 (4) ◽

pp. 501-507 ◽

Cited By ~ 74

Author(s):

Rolf Geisen ◽

Zsuzsanna Mayer ◽

Anja Karolewiez ◽

Paul Färber

Keyword(s):

Real Time ◽

Ochratoxin A ◽

Real Time Pcr ◽

Polyketide Synthase ◽

Penicillium Nordicum ◽

Polyketide Synthase Gene

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Faculty Opinions recommendation of Broadly targeted triplex real-time PCR detection of influenza A, B and C viruses based on the nucleoprotein gene and a novel "MegaBeacon" probe strategy.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.713998087.789552802 ◽

2012 ◽

Author(s):

Sanjay Tyagi

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Influenza A ◽

Pcr Detection ◽

Nucleoprotein Gene

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Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study

Microorganisms ◽

10.3390/microorganisms9040800 ◽

2021 ◽

Vol 9 (4) ◽

pp. 800

Author(s):

Francesca Servadei ◽

Silvestro Mauriello ◽

Manuel Scimeca ◽

Bartolo Caggiano ◽

Marco Ciotti ◽

...

Keyword(s):

Viral Load ◽

Observational Study ◽

Real Time ◽

Real Time Pcr ◽

Pcr Detection ◽

Viral Rna ◽

Clinical Documentation ◽

Post Mortem ◽

Medical Assistance ◽

Time Points

The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 h; T2 12 h; T3 24 h) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. SARS-CoV-2 viral RNA was still detectable in 70.3% of cases within 2 h after death and in 66,6% of cases up to 24 h after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 h post-mortem (T3) in comparison to that evaluated 2 h after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 h after death) from 4 subjects who were negative at T1 (2 h after death). The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.

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