Assessment of DNA damage by comet assay and fast halo assay in buccal epithelial cells of Indian women chronically exposed to biomass smoke

2011 ◽  
Vol 214 (4) ◽  
pp. 311-318 ◽  
Author(s):  
Nandan Kumar Mondal ◽  
Purba Bhattacharya ◽  
Manas Ranjan Ray
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Indian Women ◽  
Biomass Smoke ◽  
Buccal Epithelial Cells

scholarly journals Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

10.5772/62760 ◽  
2016 ◽  
Author(s):  
J. Sánchez-Alarcón ◽  
M. Milić ◽  
S. Gómez-Arroyo ◽  
J. M. R. Montiel-González ◽  
R. Valencia-Quintana
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Buccal Epithelial Cells

scholarly journals DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay

2019 ◽  
Vol 18 ◽  
pp. e191430
Author(s):  
Garima Rawat ◽  
Aadithya B Urs ◽  
Anita Chakravarti ◽  
Priya Kumar
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Oral Submucous Fibrosis ◽  
Tail Length ◽  
Buccal Cells ◽  
Buccal Epithelial Cells ◽  
Significant Difference ◽  
Potentially Malignant ◽  
The Mean

Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of DNA damage; lymphocytesare the most commonly used cells for performing comet assay. Utilisation of buccal epithelial cells in comet assay can be a minimally invasive and rapid method.  The present study compared the efficacy of comet assay in assessing DNA damage in buccal cells over peripheral blood leucocytes (PBLs) in oral potentially malignant and malignant disorders. Methods: The study included fifty five patients each of Leukoplakia, Oral Submucous Fibrosis (OSMF) and OSCC along with fifty five healthy individuals as control. Buccal epithelial cells were collected from all the selected subjects. DNA damage was evaluated bymeasuring the mean tail length (µm). Results: A significantly increased mean tail length (µm) and higher DNA damage were found in OSCC (26.1096 + 1.84355) and there was a progressive stepwise increase in mean tail length from control(8.4982 + 0.93307) to PMD [leukoplakia (14.6105 + 0.71857); OSMF (12.5009 + 1.12694)] to OSCC.The mean tail length in different habit groups was greater than controls, though no significant difference was noted between habit groups. The mean tail length of buccal cells was significantly greater than the mean tail length of PBLs in all study groups and controls. Conclusion: Hence, use of comet assay on buccal epithelial cells can prove to be beneficiary for evaluation of DNA damage.

scholarly journals Ocular Cell Lines and Genotoxicity Assessment

2020 ◽  
Vol 17 (6) ◽  
pp. 2046 ◽  
Author(s):  
Eliana B. Souto ◽  
Joana R. Campos ◽  
Raquel Da Ana ◽  
Carlos Martins-Gomes ◽  
Amélia M. Silva ◽  
...  
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Cell Lines ◽  
Human Body ◽  
Ex Vivo ◽  
Drug Formulation ◽  
Screening Tests ◽  
Published Data ◽  
Special Focus

Genotoxicity screening tests aim to evaluate if and to what extent a compound in contact with the human body (e.g., a drug molecule, a compound from the environment) interacts with DNA. The comet assay is a sensitive method used to predict the risk of DNA damage in individual cells, as it quantifies the tape breaks, being the alkaline version (pH > 13) the most commonly used in the laboratory. Epithelial cells serve as biomatrices in genotoxicity assessments. As ca. 80% of solid cancers are of epithelial origin, the quantification of the DNA damage upon exposure of epithelial cells to a drug or drug formulation becomes relevant. Comet assays run in epithelial cells also have clinical applications in human biomonitoring, which assesses whether and to what extent is the human body exposed to environmental genotoxic compounds and how such exposure changes over time. Ocular mucosa is particularly exposed to environmental assaults. This review summarizes the published data on the genotoxicity assessment in estimating DNA damage in epithelial cells with a special focus on ocular cell lines. General comet assay procedures for ex vivo and in vivo epithelium samples are also described.

scholarly journals DNA Damage Assessment in Buccal Epithelial Cells as Marker of Oral Cancer amongst Smokeless Tobacco (Khaini) and Alcohol Users

2019 ◽  
Vol 04 (11) ◽  
pp. 773-780
Author(s):  
Sarim Ahmad ◽  
Seema Sharma ◽  
Ahmed SS ◽  
Yasar Hasan Siddique ◽  
Uroosa Tabassum ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oral Cancer ◽  
Epithelial Cells ◽  
Smokeless Tobacco ◽  
Damage Assessment ◽  
Buccal Epithelial Cells ◽  
Alcohol Users

Investigation of Cellular Function and DNA Integrity during 2D in vitro Culture of Human Salivary Gland Epithelial Cells

2019 ◽  
Vol 208 (1-2) ◽  
pp. 66-75
Author(s):  
Marc Burghartz ◽  
Johannes Taeger ◽  
Marco Metzger ◽  
Agmal Scherzad ◽  
Thomas Gehrke ◽  
...  
Keyword(s):  
Dna Damage ◽  
Salivary Gland ◽  
Epithelial Cells ◽  
Comet Assay ◽  
In Vitro Culture ◽  
Cellular Function ◽  
Dna Integrity ◽  
Human Salivary Gland ◽  
Salivary Gland Epithelial Cells

In vitro culture of human salivary gland epithelial cells (SGEC) is still a challenge. A high quantity and quality of cells are needed for the cultivation of 3D matrices. Furthermore, it is known that DNA damage is supposed to be an important factor involved in carcinogenesis. This study investigates cellular function and DNA integrity of human SGEC during 3 passage steps in 2 groups (group 1: n = 10; group 2: n = 9). Cellular function was analyzed by immunofluorescence, transmission electron microscopy (TEM), and quantitative real-time polymerase chain reaction (qPCR). DNA integrity was tested via the comet assay. Immunohistochemistry and qPCR results showed stable α-amylase and pan-cytokeratin levels; TEM revealed functional cells; and no significant DNA damage could be detected in the comet assay during 3 culture steps. The study shows that not only at cellular but also at DNA level human SGEC can be safely quantified over 3 passages for preclinical tissue engineering without loss of differentiation and function.

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