scholarly journals Molecular detection of Mycoplasma pneumoniae among patients with severe respiratory and influenza-like illness in South Africa, 2012-2013

2014 ◽  
Vol 21 ◽  
pp. 345 ◽  
Author(s):  
M. Carrim ◽  
N. Wolter ◽  
M. du Plessis ◽  
L. de Gouveia ◽  
S. Walaza ◽  
...  
Keyword(s):  
South Africa ◽  
Mycoplasma Pneumoniae ◽  
Molecular Detection ◽  
Influenza Like Illness

scholarly journals Influenza Epidemiology and Vaccine Effectiveness among Patients with Influenza-Like Illness, Viral Watch Sentinel Sites, South Africa, 2005–2009

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94681 ◽  
Author(s):  
Genevie M. Ntshoe ◽  
Johanna M. McAnerney ◽  
Stefano Tempia ◽  
Lucille Blumberg ◽  
Jocelyn Moyes ◽  
...  
Keyword(s):  
South Africa ◽  
Vaccine Effectiveness ◽  
Influenza Like Illness

scholarly journals Molecular Detection of Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia Requiring Hospitalization

2001 ◽  
Vol 39 (3) ◽  
pp. 1184-1186 ◽  
Author(s):  
J. W. Dorigo-Zetsma ◽  
R. P. Verkooyen ◽  
H. P. van Helden ◽  
H. van der Nat ◽  
J. M. van den Bosch
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Molecular Detection ◽  
Community Acquired Pneumonia

The First Molecular Detection of Aichi Virus 1 in Raw Sewage and Mussels Collected in South Africa

2018 ◽  
Vol 11 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Oikwathaile Onosi ◽  
Nicole S. Upfold ◽  
Michael D. Jukes ◽  
Garry A. Luke ◽  
Caroline Knox
Keyword(s):  
South Africa ◽  
Molecular Detection ◽  
Aichi Virus

scholarly journals Improved performance of saliva for the detection of the B.1.351 variant in South Africa

2021 ◽  
Author(s):  
Chun Yat Chu ◽  
Gert Marais ◽  
Christoffel Opperman ◽  
Deelan Doolabh ◽  
Arash Iranzadeh ◽  
...  
Keyword(s):  
South Africa ◽  
Marked Improvement ◽  
Molecular Detection ◽  
Cape Town ◽  
The Novel ◽  
Wild Type ◽  
Rt Pcr ◽  
Diagnostic Laboratory ◽  
Percent Agreement ◽  
Improved Performance

Assessment of the unknown performance of saliva for the detection of the novel SARS-CoV-2 variant of concern (VOC) B.1.351 (501Y.V2) lineage is essential as saliva has been shown to be an equivalent, less invasive and a less costly alternative to nasopharyngeal swabs for the molecular detection of SARS-CoV-2 infection in pre-variant studies. Between 1st August 2020 and 16th January 2021, we enrolled 410 eligible ambulatory participants who presented to Groote Schuur Hospital (GSH) in Cape Town, South Africa for SARS-CoV-2 testing. Of these, 300 were enrolled prior to, and 110 after, the initial detection and replacement of wild-type by the B.1.351 variant. All participants provided a supervised self-collected mid-turbinate (MT) and saliva (SA) swab, in addition to the standard HCW collected NP swab which were all tested by RT-PCR in an accredited diagnostic laboratory. Positive percent agreement to NP swab for SA swabs pre- and post-variant were 51.5% and 72.5% respectively while these values for MT swabs were 75.8% and 77.5%. The negative percent agreement for all swab types during all periods was >98%. The basis for this marked improvement of SA swabs as a diagnostic sample for B.1.351 virus is still being investigated.

scholarly journals Molecular detection of Babesia rossi and Hepatozoon sp. in African wild dogs (Lycaon pictus) in South Africa

2008 ◽  
Vol 157 (1-2) ◽  
pp. 123-127 ◽  
Author(s):  
Paul Tshepo Matjila ◽  
Andrew L. Leisewitz ◽  
Frans Jongejan ◽  
Henk J. Bertschinger ◽  
Barend L. Penzhorn
Keyword(s):  
South Africa ◽  
Molecular Detection ◽  
Lycaon Pictus ◽  
African Wild Dogs ◽  
Babesia Rossi ◽  
Wild Dogs

scholarly journals Preharvest Detection and Postharvest Incidence of Phlyctema vagabunda on ‘Cripps Pink’ Apples in South Africa

Plant Disease ◽  
2020 ◽  
Vol 104 (3) ◽  
pp. 841-846 ◽  
Author(s):  
Alana Den Breeyen ◽  
Jessica Rochefort ◽  
Andre Russouw ◽  
Julia Meitz-Hopkins ◽  
Cheryl L. Lennox
Keyword(s):  
South Africa ◽  
Management Practices ◽  
Molecular Detection ◽  
Disease Incidence ◽  
Postharvest Disease ◽  
Western Cape ◽  
Pome Fruit ◽  
Current Management ◽  
Detection Tool ◽  
Fruit Growing

Phlyctema vagabunda is responsible for significant postharvest losses in ‘Cripps Pink’ apples in South Africa. The first objective of this study was to determine the presence and incidence of P. vagabunda on stored commercial ‘Cripps Pink’ apple in five major pome fruit growing regions in the Western Cape. As the fungus remains latent until postharvest, the second objective was to develop a rapid molecular detection tool to determine the presence of P. vagabunda on asymptomatic ‘Cripps Pink’ apples from two commercial orchards. Postharvest disease incidence in ‘Cripps Pink’ apples in the Western Cape ranged from 0 to 73% in the 2010/2011, 0 to 6% in 2011/2012, and 0 to 30% in 2012/2013 seasons. P. vagabunda spores were also detected on ‘Cripps Pink’ fruit from December to February and from March to April. In December and January, P. vagabunda spores were detected on the ‘Hillieri’ crab apple pollinator. The knowledge that P. vagabunda conidia are present on the surface of ‘Cripps Pink’ apples and ‘Hillieri’ crab apples during the growing season could be applied to improving current management practices in the orchards.

scholarly journals Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

2020 ◽  
Vol 91 ◽  
Author(s):  
Tsepo A. Ramatla ◽  
Nthabiseng Mphuthi ◽  
Taole Ramaili ◽  
Moeti O. Taioe ◽  
Oriel M.M. Thekisoe ◽  
...  
Keyword(s):  
South Africa ◽  
Virulence Genes ◽  
Molecular Detection ◽  
Salmonella Spp

scholarly journals Genomics-Informed Molecular Detection of Xanthomonas vasicola pv. vasculorum Strains Causing Severe Bacterial Leaf Streak of Corn

2020 ◽  
Vol 110 (6) ◽  
pp. 1174-1179 ◽  
Author(s):  
Michael J. Stulberg ◽  
Gem Santillana ◽  
David J. Studholme ◽  
Beth Kasiborski ◽  
Mary Ortiz-Castro ◽  
...  
Keyword(s):  
United States ◽  
South Africa ◽  
Molecular Detection ◽  
Severe Disease ◽  
The United States ◽  
Whole Genome ◽  
Propidium Monoazide ◽  
Bacterial Leaf Streak ◽  
Specific Subset ◽  
Taqman Qpcr

Xanthomonas vasicola pv. vasculorum (syn. X. campestris pv. vasculorum) was initially identified as the causal agent of bacterial leaf streak of corn in South Africa. The pathovar vasculorum causes disease on sugarcane and corn, but a subset of these strains was noted for its increased disease severity in corn. This subset was reclassified as X. campestris pv. zeae in the early 1990s and was found to have slightly different biochemical and genetic properties than isolates from sugarcane. There has been an emergence of X. campestris pv. zeae-like strains of X. vasicola pv. vasculorum in both the United States and Argentina since 2010. We performed whole genome sequencing on U.S. isolates to confirm their identity. Informed by comparative genomics, we then developed specific TaqMan qPCR and loop-mediated isothermal amplification (LAMP) assays for the detection of this specific subset of X. vasicola pv. vasculorum strains. The qPCR 4909 assay was tested against 27 xanthomonads (diverse representation), 32 DNA extractions from corn leaves confirmed as positive or negative for the bacterium, 41 X. vasicola pv. vasculorum isolates from corn in the United States and Argentina, and 31 additional bacteria associated with corn, sugarcane, or sorghum. In all cases the assay was shown to be specific for the X. vasicola pv. vasculorum isolates that cause more severe disease on corn. We then tested the LAMP 166 assay against the 27 xanthomonads and 32 corn leaf DNA samples, and we found this assay was also specific for this subset of X. vasicola pv. vasculorum isolates. We also developed a live/dead cells distinction protocol using propidium monoazide prior to DNA extraction for analyzing seed washes using these assays. These two detection assays can be useful for both diagnosticians and researchers to specifically identify the X. vasicola pv. vasculorum isolates that cause more severe symptoms on corn.

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