scholarly journals Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

2018 ◽  
Vol 73 ◽  
pp. 69-71 ◽  
Author(s):  
Praphathip Eamsobhana ◽  
Anchalee Tungtrongchitr ◽  
Darawan Wanachiwanawin ◽  
Hoi-Sen Yong
Keyword(s):  
Serological Diagnosis ◽  
Immunochromatographic Test

The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia

2018 ◽  
Vol 117 (9) ◽  
pp. 2913-2919 ◽  
Author(s):  
Daiki Mizushima ◽  
Tovuu Amgalanbaatar ◽  
Batdorj Davaasuren ◽  
Nthatisi Innocentia Molefe ◽  
Banzragch Battur ◽  
...  
Keyword(s):  
Rural Areas ◽  
Serological Diagnosis ◽  
Immunochromatographic Test

THE USE OF SYNTHETIC GLYCOCONJUGATES AS COMPONENTS OF THE IMMUNOCHROMATOGRAPHIC TEST FOR RAPID SEROLOGICAL DIAGNOSIS OF LEPROSY

2020 ◽  
Vol 65 (5) ◽  
pp. 289-293
Author(s):  
A. G. Korolyova-Ushakova ◽  
E. V. Baranova ◽  
S. G. Ignatov ◽  
G. N. Fedyukina ◽  
P. V. Solov’ev ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serological Diagnosis ◽  
Diagnostic Sensitivity ◽  
Immunochromatographic Test ◽  
Antigenic Component ◽  
Healthy Donors ◽  
Leprosy Patients

The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest). The data obtained with sera of leprosy patients, patients who have been in contact with leprosy, and healthy donors indicate that the most promising antigenic component is a BSA conjugate with two synthetic epitopes - a disaccharide derivative of PGL-1 and a branched hexasaccharide fragment of LAM. The leprosy LF serotest with such glycoconjugate demonstrated the greatest diagnostic sensitivity for main forms of leprosy - paucibacillary (PB) and multibacillary (MB)

Rapid serological diagnosis of melioidosis: an evaluation of a prototype immunochromatographic test

Pathology ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 169-171 ◽  
Author(s):  
Seong C. Chuah ◽  
Glenda Gilmore ◽  
Robert E. Norton
Keyword(s):  
Serological Diagnosis ◽  
Immunochromatographic Test

scholarly journals Evaluation of a Rapid Device for Serological Diagnosis of Leishmania infantum Infection in Dogs as an Alternative to Immunofluorescence Assay and Western Blotting

2013 ◽  
Vol 20 (5) ◽  
pp. 657-659 ◽  
Author(s):  
E. Ferroglio ◽  
S. Zanet ◽  
W. Mignone ◽  
M. Poggi ◽  
A. Trisciuoglio ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Western Blotting ◽  
Indirect Immunofluorescence ◽  
Leishmania Infantum ◽  
Immunofluorescence Assay ◽  
Serological Diagnosis ◽  
Immunochromatographic Test ◽  
Serum Samples ◽  
Content Type ◽  
Test Speed

ABSTRACTIn this study, we compared a rapid immunochromatographic test (Speed Leish K; BVT Groupe Virbac, La Seyne sur Mer, France) with an indirect immunofluorescence assay (IFAT) and Western blotting (WB) for the detection ofLeishmania infantumantibodies in dogs. A total of 250 serum samples were collected from 125L. infantum-positive and 125L. infantum-negative dogs. Among the positive samples, 81 were strongly positive at low IFAT dilutions, while 44 were low-reactivity sera (IFAT titers, 1:40 to 1:80). The sensitivity and specificity of the Speed Leish K were 96.3% and 100%, respectively, compared with those of the IFAT. When IFAT low-reactivity sera (titers, 1:40 or 1:80) were tested with the Speed Leish K, using WB results as a reference, the sensitivities were 93.75% for sera with a 1:80 titer and 73.33% for sera with a 1:40 titer, and the specificity was 100%. The Speed Leish K is easy to use and performs well, so it can be considered a quick and reliable tool for the diagnosis ofL. infantuminfection in dogs.

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