Evaluation of immunochromatographic test in early serological diagnosis of dengue fever

The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest). The data obtained with sera of leprosy patients, patients who have been in contact with leprosy, and healthy donors indicate that the most promising antigenic component is a BSA conjugate with two synthetic epitopes - a disaccharide derivative of PGL-1 and a branched hexasaccharide fragment of LAM. The leprosy LF serotest with such glycoconjugate demonstrated the greatest diagnostic sensitivity for main forms of leprosy - paucibacillary (PB) and multibacillary (MB)

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Rapid serological diagnosis of melioidosis: an evaluation of a prototype immunochromatographic test

Pathology ◽

10.1080/00313020500058516 ◽

2005 ◽

Vol 37 (2) ◽

pp. 169-171 ◽

Cited By ~ 6

Author(s):

Seong C. Chuah ◽

Glenda Gilmore ◽

Robert E. Norton

Keyword(s):

Serological Diagnosis ◽

Immunochromatographic Test

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Prevalence of dengue virus infection among population of Bhaili visiting tertiary health institution; Chhattisgarh

Asian Journal of Medical Sciences ◽

10.3126/ajms.v11i2.27345 ◽

2020 ◽

Vol 11 (2) ◽

pp. 30-34

Author(s):

Dhruba Hari Chandi

Keyword(s):

Virus Infection ◽

Early Detection ◽

Dengue Virus ◽

Dengue Fever ◽

Dengue Infection ◽

Rapid Test ◽

Age Group ◽

Immunochromatographic Test ◽

Dengue Virus Infection ◽

Health Institution

Background: Dengue fever, dengue haemorrhagic fever and dengue shock syndrome of infection is distinct clinical forms which is caused by Dengue Virus that belong to a member of the Flaviviridae family. It is most important arthropod borne viral disease that causes morbidity and mortality. Dengue virus infection is a major public health problem growing in worldwide and it is estimated about 2.5 billion people of world are at risk of this infection. In India several parts of the country it is an endemic disease. Aims and Objective: The current work is an attempt to review current perspectives of dengue infection among population of Bhilai visiting tertiary health institution. In study also we have compared the serological profiles of the dengue cases. Material and Methods: Samples received were performing retrospective analysis in the department of microbiology and process in the departmental microbiology laboratory obtaining during monsoon season. The duration of fever (in days) and other relevant clinical information were recorded from the requisition form. Rapid test for dengue as NS1Ag, IgG and IgM test were performed in accordance with the manufacturer’s instructions. The dengue NS1Ag, IgG and IgM rapid test is an in vitro immunochromatographic test (ICT) which is a onestep assay designed for the qualitative determination of dengue NS1Ag, IgG and IgM in human serum for the diagnosis dengue infection. Result: During the study period total 1308 serum samples were collected from suspected Dengue fever patients. Among 1308 samples 412(31.5%) samples were found to be positive dengue fever. In the month of August maximum number of sample were received. Out of 1308 patients with dengue suspected patients 792(60.6%) were male and 516(39.4%) were female. Among 60.6% of male 28.2% were positive and out of 39.4% of female 36.6% were positive for dengue. Age between 10-20 years old (55.83%) patients were most affected age group and 70-80 years old (8.77%) age group were least affected. Among the dengue positive patients, dengue parameter associated with thrombocytopenia was also recorded which showed that there was no significant difference between the parameters in relation to thrombocytopenia. Conclusion: Especially in developing countries like India where there are poor resources for diagnosis of dengue infection, the sensitivity of these tests is more than immunochromatographic Test (ICT). By NS1 assay early detection of dengue infection can help in early confirmation and management of this before its gets complicate. Therefore commercial available dengue NS1 antigen test kits provided additional laboratory diagnostic tool for early detection of dengue.

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Evaluation of a Rapid Device for Serological Diagnosis of Leishmania infantum Infection in Dogs as an Alternative to Immunofluorescence Assay and Western Blotting

Clinical and Vaccine Immunology ◽

10.1128/cvi.00719-12 ◽

2013 ◽

Vol 20 (5) ◽

pp. 657-659 ◽

Cited By ~ 9

Author(s):

E. Ferroglio ◽

S. Zanet ◽

W. Mignone ◽

M. Poggi ◽

A. Trisciuoglio ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Western Blotting ◽

Indirect Immunofluorescence ◽

Leishmania Infantum ◽

Immunofluorescence Assay ◽

Serological Diagnosis ◽

Immunochromatographic Test ◽

Serum Samples ◽

Content Type ◽

Test Speed

ABSTRACTIn this study, we compared a rapid immunochromatographic test (Speed Leish K; BVT Groupe Virbac, La Seyne sur Mer, France) with an indirect immunofluorescence assay (IFAT) and Western blotting (WB) for the detection ofLeishmania infantumantibodies in dogs. A total of 250 serum samples were collected from 125L. infantum-positive and 125L. infantum-negative dogs. Among the positive samples, 81 were strongly positive at low IFAT dilutions, while 44 were low-reactivity sera (IFAT titers, 1:40 to 1:80). The sensitivity and specificity of the Speed Leish K were 96.3% and 100%, respectively, compared with those of the IFAT. When IFAT low-reactivity sera (titers, 1:40 or 1:80) were tested with the Speed Leish K, using WB results as a reference, the sensitivities were 93.75% for sera with a 1:80 titer and 73.33% for sera with a 1:40 titer, and the specificity was 100%. The Speed Leish K is easy to use and performs well, so it can be considered a quick and reliable tool for the diagnosis ofL. infantuminfection in dogs.

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