scholarly journals Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test

2015 ◽  
pp. 33-39 ◽  
Author(s):  
Tomomi Takano ◽  
Tsutomu Hohdatsu
Keyword(s):  
Serological Diagnosis ◽  
Immunochromatographic Test ◽  
Coronavirus Infection ◽  
Feline Coronavirus

scholarly journals Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests

2014 ◽  
Vol 196 ◽  
pp. 1-6 ◽  
Author(s):  
Tomomi Takano ◽  
Yuka Ishihara ◽  
Masafumi Matsuoka ◽  
Shoko Yokota ◽  
Yukie Matsuoka-Kobayashi ◽  
...  
Keyword(s):  
Serological Diagnosis ◽  
Nucleocapsid Proteins ◽  
Coronavirus Infection ◽  
Feline Coronavirus

scholarly journals The Population Diversity of Candidate Genes for Resistance/Susceptibility to Coronavirus Infection in Domestic Cats: An Inter-Breed Comparison

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 778
Author(s):  
Jana Bubenikova ◽  
Leona Vychodilova ◽  
Karla Stejskalova ◽  
Jan Futas ◽  
Jan Oppelt ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Single Gene ◽  
Differential Susceptibility ◽  
Snp Markers ◽  
Population Diversity ◽  
Population Substructure ◽  
Domestic Cats ◽  
Coronavirus Infection ◽  
Breed Comparison ◽  
Feline Coronavirus

Feline coronavirus (FCoV) is a complex pathogen causing feline infectious peritonitis (FIP). Host genetics represents a factor contributing to the pathogenesis of the disease. Differential susceptibility of various breeds to FIP was reported with controversial results. The objective of this study was to compare the genetic diversity of different breeds on a panel of candidate genes potentially affecting FCoV infection. One hundred thirteen cats of six breeds were genotyped on a panel of sixteen candidate genes. SNP allelic/haplotype frequencies were calculated; pairwise FST and molecular variance analyses were performed. Principal coordinate (PCoA) and STRUCTURE analyses were used to infer population structure. Interbreed differences in allele frequencies were observed. PCoA analysis performed for all genes of the panel indicated no population substructure. In contrast to the full marker set, PCoA of SNP markers associated with FCoV shedding (NCR1 and SLX4IP) showed three clusters containing only alleles associated with susceptibility to FCoV shedding, homozygotes and heterozygotes for the susceptibility alleles, and all three genotypes, respectively. Each cluster contained cats of multiple breeds. Three clusters of haplotypes were identified by PCoA, two clusters by STRUCTURE. Haplotypes of a single gene (SNX5) differed significantly between the PCoA clusters.

scholarly journals Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

2018 ◽  
Vol 73 ◽  
pp. 69-71 ◽  
Author(s):  
Praphathip Eamsobhana ◽  
Anchalee Tungtrongchitr ◽  
Darawan Wanachiwanawin ◽  
Hoi-Sen Yong
Keyword(s):  
Serological Diagnosis ◽  
Immunochromatographic Test

scholarly journals Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wayne Vuong ◽  
Muhammad Bashir Khan ◽  
Conrad Fischer ◽  
Elena Arutyunova ◽  
Tess Lamer ◽  
...  
Keyword(s):  
Drug Target ◽  
Covalent Modification ◽  
Human Coronavirus ◽  
Feline Infectious Peritonitis ◽  
Drug Candidates ◽  
Main Protease ◽  
Coronavirus Infection ◽  
Reversible Formation ◽  
Nanomolar Range ◽  
Feline Coronavirus

Abstract The main protease, Mpro (or 3CLpro) in SARS-CoV-2 is a viable drug target because of its essential role in the cleavage of the virus polypeptide. Feline infectious peritonitis, a fatal coronavirus infection in cats, was successfully treated previously with a prodrug GC376, a dipeptide-based protease inhibitor. Here, we show the prodrug and its parent GC373, are effective inhibitors of the Mpro from both SARS-CoV and SARS-CoV-2 with IC50 values in the nanomolar range. Crystal structures of SARS-CoV-2 Mpro with these inhibitors have a covalent modification of the nucleophilic Cys145. NMR analysis reveals that inhibition proceeds via reversible formation of a hemithioacetal. GC373 and GC376 are potent inhibitors of SARS-CoV-2 replication in cell culture. They are strong drug candidates for the treatment of human coronavirus infections because they have already been successful in animals. The work here lays the framework for their use in human trials for the treatment of COVID-19.

scholarly journals Antiviral Effects of Hydroxychloroquine and Type I Interferon on In Vitro Fatal Feline Coronavirus Infection

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 576 ◽  
Author(s):  
Tomomi Takano ◽  
Kumi Satoh ◽  
Tomoyoshi Doki ◽  
Taishi Tanabe ◽  
Tsutomu Hohdatsu
Keyword(s):  
Viral Infections ◽  
Viral Disease ◽  
High Morbidity ◽  
Type I ◽  
Feline Infectious Peritonitis ◽  
Antiviral Effects ◽  
Immune Mediated ◽  
Coronavirus Infection ◽  
Feline Coronavirus

Feline infectious peritonitis (FIP) is a viral disease with a high morbidity and mortality by the FIP virus (FIPV, virulent feline coronavirus). Several antiviral drugs for FIP have been identified, but many of these are expensive and not available in veterinary medicine. Hydroxychloroquine (HCQ) is a drug approved by several countries to treat malaria and immune-mediated diseases in humans, and its antiviral effects on other viral infections (e.g., SARS-CoV-2, dengue virus) have been confirmed. We investigated whether HCQ in association with interferon-ω (IFN-ω) is effective for FIPV in vitro. A total of 100 μM of HCQ significantly inhibited the replication of types I and II FIPV. Interestingly, the combination of 100 μM of HCQ and 104 U/mL of recombinant feline IFN-ω (rfIFN-ω, veterinary registered drug) increased its antiviral activity against type I FIPV infection. Our study suggested that HCQ and rfIFN-ω are applicable for treatment of FIP. Further clinical studies are needed to verify the combination of HCQ and rIFN-ω will be effective and safe treatment for cats with FIP.

scholarly journals Differential effect of cholesterol on type I and II feline coronavirus infection

2015 ◽  
Vol 161 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Tomomi Takano ◽  
Yui Satomi ◽  
Yuu Oyama ◽  
Tomoyoshi Doki ◽  
Tsutomu Hohdatsu
Keyword(s):  
Differential Effect ◽  
Type I ◽  
Coronavirus Infection ◽  
Feline Coronavirus

scholarly journals Feline coronavirus infection

2021 ◽  
Vol 273 ◽  
pp. 02025
Author(s):  
Aleksey Ermakov ◽  
Tatyana Lipilkina ◽  
Pavel Lipilkin ◽  
Igor Popov
Keyword(s):  
Primary Prevention ◽  
Therapeutic Interventions ◽  
Feline Infectious Peritonitis ◽  
Clinical Diagnostic ◽  
Coronavirus Infection ◽  
Near Future ◽  
Feline Coronavirus ◽  
Prevention And Therapy

The main feature of feline coronavirus infection is its manifestation in the form of peritonitis. Feline infectious peritonitis is a highly lethal disease that lacks primary prevention and therapy. Therefore, feline infectious peritonitis is an epizootic problem in the near future. In our review, we demonstrate the current clinical, diagnostic, and therapeutic interventions for feline infectious peritonitis, as well as hypotheses of origin.

scholarly journals Feline Coronavirus infections and feline infectious peritonitis

2018 ◽  
Vol 50 (3) ◽  
pp. 199
Author(s):  
A. TZIVARA (Α. ΤΖΙΒΑΡΑ) ◽  
S. K. KRITAS (Σ.Κ. ΚΡΗΤΑΣ)
Keyword(s):  
Gastrointestinal Disease ◽  
Clinical Signs ◽  
Asymptomatic Infection ◽  
Temperature Sensitive ◽  
Feline Infectious Peritonitis ◽  
Antibody Titers ◽  
Coronavirus Infection ◽  
Different Strains ◽  
Physical Findings ◽  
Feline Coronavirus

Cats are susceptible to infection with several different strains of feline Coronavirus. Depending on the involved strain, clinical signs may range from asymptomatic infection to gastrointestinal disease or fibrinous serositis and disseminated vasculitis, commonly known as feline infectious peritonitis (FIP). Excretion of virus by infected cats into the environment occurs by faeces, oronasal secretions and urine. The feline coronaviruses are rapidly inactivated by most disinfectants. Clinical diagnosis of Coronavirus infection is made by evaluating the case history, physical findings, laboratory results, Coronavirus antibody titers and tissue biopsy. A temperature-sensitive feline infectious peritonitis virus vaccine has become available for healthy 16 week of age or older cats.

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