Epidemiology and molecular characterization of rotavirus A in bats and rhesus macaques at human-wildlife interfaces in Bangladesh

INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.

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Detection and Molecular Characterization of Rotavirus and Picobirnavirus in Wild Avians from Amazon Forest

10.1101/2020.09.15.297689 ◽

2020 ◽

Author(s):

José Wandilson Barboza Duarte Júnior ◽

Elaine Hellen Nunes Chagas ◽

Ana Carolina Silva Serra ◽

Lizandra Caroline dos Santos Souto ◽

Edvaldo Tavares da Penha Júnior ◽

...

Keyword(s):

Molecular Characterization ◽

Clinical Signs ◽

Wild Birds ◽

Interspecies Transmission ◽

Amazon Forest ◽

Rotavirus A ◽

Exotic Birds ◽

Veterinary Hospital ◽

Nsp3 Gene

ABSTRACTThe present study reports the detection and molecular characterization of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG) and picobirnavirus (PBV) in fecal specimens of wild and exotic birds (n = 23) from different cities of Pará state, which were hospitalized at Veterinary Hospital of the Federal University of Pará, Brazil, between January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, equine-like G3 and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in 1 of the 23 samples tested and was classified in the Genogroup I. The findings represent the first report of the circulation of RVA, RVD, RVF, RVG and PBV genotypes in wild birds in Brazil and suggest the possible interspecies transmission of RVs and PBVs.

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Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

Transboundary and Emerging Diseases ◽

10.1111/tbed.13431 ◽

2019 ◽

Vol 67 (2) ◽

pp. 956-966 ◽

Cited By ~ 1

Author(s):

Ariful Islam ◽

Mohammad Enayet Hossain ◽

Najmul Haider ◽

Melinda K. Rostal ◽

Sanjoy Kumar Mukharjee ◽

...

Keyword(s):

Molecular Characterization ◽

Macaca Mulatta ◽

Rhesus Macaques ◽

Group A Rotavirus ◽

Group A

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Molecular characterization of porcine rotavirus A from India revealing zooanthroponotic transmission

Animal Biotechnology ◽

10.1080/10495398.2020.1868486 ◽

2021 ◽

pp. 1-13

Author(s):

Gazanfar Abass ◽

Zunjar Baburao Dubal ◽

Kaushal K. Rajak ◽

Balasaheb M. Kale ◽

Abhay Raorane ◽

...

Keyword(s):

Molecular Characterization ◽

Rotavirus A ◽

Porcine Rotavirus

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Molecular Characterization of Rotavirus A Associated with Outbreaks of Acute Gastroenteritis in Sarawak in 2001 and 2007

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2008.05.229 ◽

2008 ◽

Vol 12 ◽

pp. e91-e92

Author(s):

M.S. Sum ◽

D. Perera ◽

N. Ramji ◽

F. Elie ◽

M.J. Cardosa

Keyword(s):

Molecular Characterization ◽

Acute Gastroenteritis ◽

Rotavirus A

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Detection and molecular characterization of rotavirus a antigen in fecal samples from diarrheic and non-diarrheic pigs in Maputo city and province, Southern of Mozambique

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2018.11.179 ◽

2019 ◽

Vol 79 ◽

pp. 70

Author(s):

S.S. Boene ◽

E. João ◽

B. Munlela ◽

E. Nabetse ◽

A. Cala ◽

...

Keyword(s):

Molecular Characterization ◽

Fecal Samples ◽

Rotavirus A ◽

Maputo City

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Non-structural Enterotoxin (NSP4) Gene based Molecular Characterization of Caprine and Ovine Rotavirus A, India

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.4.09 ◽

2020 ◽

Vol 14 (4) ◽

pp. 2303-2311

Author(s):

Shubhankar Sircar ◽

Prashant Kumar ◽

Mohd Ikram Ansari ◽

Sudipta Bhat ◽

Jobin Jose Kattoor ◽

...

Keyword(s):

Molecular Characterization ◽

Genetic Characterization ◽

Full Length ◽

Viral Gastroenteritis ◽

Enterotoxin Gene ◽

Nsp4 Gene ◽

Future Studies ◽

Rotavirus A ◽

Large Animals

Rotavirus A (RVA) causes viral gastroenteritis in humans and animals, including calves, piglets, and foals. The current study reports the genetic characterization of the full-length enterotoxin gene, NSP4, from caprine and ovine species. Upon characterizing eight full-length NSP4 genes by sequencing, it was found that the four caprine and three ovine RVAs NSP4 genes are of E2 genotype and the sole ovine RVA isolate was found to be of E1 genotype. In the sequence and phyloanalysis of the NSP4 gene the seven E2 genotypes clustered with bovine, human, and caprine isolates from India and Bangladesh, respectively. The E1 genotype of ovine RVA was closer to human RVA isolate from India. The nucleotide per cent identity analysis revealed that all E2 genotype strains of caprine and ovine species ranged from 88.4% to 90.4% and it was found common to both the reference human RVA isolates DS-1 and AU-1. Whereas, the E1 genotype ovine strain clustered with human RVA isolates with 93.1% nucleotide per cent identity. The RVA strains circulating in caprine and ovine populations may share a common origin which is usually found in artiodactyl species because humans share a common dwelling with animals. Future studies are needed to confirm these findings of their relationship with humans and large animals.

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