Analysis of Chromatin Opening in Heterochromatic Non-Small Cell Lung Cancer Tumor-Initiating Cells in Relation to DNA-Damaging Antitumor Treatment

2018 ◽  
Vol 100 (1) ◽  
pp. 174-187 ◽  
Author(s):  
Mina Eriksson ◽  
Petra Hååg ◽  
Beata Brzozowska ◽  
Magdalena Lipka ◽  
Halina Lisowska ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Tumor Initiating Cells ◽  
Cancer Tumor ◽  
Chromatin Opening

scholarly journals Glycine Decarboxylase Activity Drives Non-Small Cell Lung Cancer Tumor-Initiating Cells and Tumorigenesis

Cell ◽  
2012 ◽  
Vol 148 (5) ◽  
pp. 1066 ◽  
Author(s):  
Wen Cai Zhang ◽  
Ng Shyh-Chang ◽  
He Yang ◽  
Amit Rai ◽  
Shivshankar Umashankar ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Decarboxylase Activity ◽  
Glycine Decarboxylase ◽  
Small Cell Lung ◽  
Tumor Initiating Cells ◽  
Cancer Tumor

scholarly journals Glycine Decarboxylase Activity Drives Non-Small Cell Lung Cancer Tumor-Initiating Cells and Tumorigenesis

Cell ◽  
2012 ◽  
Vol 148 (1-2) ◽  
pp. 259-272 ◽  
Author(s):  
Wen Cai Zhang ◽  
Ng Shyh-Chang ◽  
He Yang ◽  
Amit Rai ◽  
Shivshankar Umashankar ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Decarboxylase Activity ◽  
Glycine Decarboxylase ◽  
Small Cell Lung ◽  
Tumor Initiating Cells ◽  
Cancer Tumor

Phosphoprotein analysis reveals MEK inhibition as a way to target non-small cell lung cancer tumor initiating cells

2014 ◽  
Vol 90 (8) ◽  
pp. 718-726 ◽  
Author(s):  
Lovisa Lundholm ◽  
Petra Hååg ◽  
Therese Juntti ◽  
Rolf Lewensohn ◽  
Kristina Viktorsson
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Tumor Initiating Cells ◽  
Mek Inhibition ◽  
Cancer Tumor

scholarly journals Corrigendum: The Emerging Role of the c-MET-HGF Axis in Non-small Cell Lung Cancer Tumor Immunology and Immunotherapy

2020 ◽  
Vol 10 ◽  
Author(s):  
Helen F. Titmarsh ◽  
Richard O'Connor ◽  
Kevin Dhaliwal ◽  
Ahsan R. Akram
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Immunology ◽  
Small Cell ◽  
Small Cell Lung ◽  
Cancer Tumor

Non-Small Cell Lung Cancer Tumor Regression During CT Based Image Guided Radiotherapy

2007 ◽  
Vol 69 (3) ◽  
pp. S500-S501
Author(s):  
R.R. Allison ◽  
T. Jenkins ◽  
F. Kiriyama ◽  
Q.Q. Taylor ◽  
G.T. Dieck ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Regression ◽  
Small Cell ◽  
Small Cell Lung ◽  
Image Guided Radiotherapy ◽  
Image Guided ◽  
Cancer Tumor

scholarly journals Change in non-small-cell lung cancer tumor size in patients treated with nintedanib plus docetaxel: analyses from the Phase III LUME-Lung 1 study

2018 ◽  
Vol Volume 11 ◽  
pp. 4573-4582 ◽  
Author(s):  
Martin Reck ◽  
Anders Mellemgaard ◽  
Silvia Novello ◽  
Pieter E Postmus ◽  
Birgit Gaschler-Markefski ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Size ◽  
Small Cell ◽  
Phase Iii ◽  
Small Cell Lung ◽  
Cancer Tumor

scholarly journals 4198 Investigating the Role of Rab27B in Non-Small Cell Lung Cancer Tumor Initiating Cells

2020 ◽  
Vol 4 (s1) ◽  
pp. 98-98
Author(s):  
Danielle Beetler ◽  
Kayleah Beltran ◽  
Kayla Lewis ◽  
Verline Justilien
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Immunoblot Analysis ◽  
Small Cell ◽  
Small Gtpase ◽  
Small Cell Lung ◽  
Tumor Initiating Cells

OBJECTIVES/GOALS: Rab27B, a small GTPase, functions in exosome formation and secretion. Rab27B is overexpressed in non-small cell lung cancer (NSCLC) and predicts patient survival; however, little is known about its importance in NSCLC cells. Here, we investigated the role of Rab27B in NSCLC tumor initiating cells. METHODS/STUDY POPULATION: Tumor initiating cells (TICs) were enriched in a panel of NSCLC cell lines using low adherence spheroid cultures. QPCR and immunoblot analysis were used to compare Rab27B mRNA and protein expression, respectively, in adherent bulk cancer cells and TIC cultures. Lentiviral-packaged short hairpin RNAs (shRNAs) were used to knockdown Rab27B in PC9 and H1299 NSCLC TICs. The effects of Rab27B knockdown on PC9 and H1299 TIC expansion, transformed growth, and invasion were analyzed by MTT cell proliferation, soft agar colony formation, and Boyden chamber assays respectively. RESULTS/ANTICIPATED RESULTS: Quantitative PCR and immunoblot analysis showed that Rab27B expression is elevated in NSCLC TICs when compared to adherent bulk cancer cells. Efficient knockdown of Rab27B was achieved in PC9 and H1299 NSCLC TICs using two independent shRNA constructs. Rab27B knockdown cells exhibited decreased expansion as spheroid cultures, transformed growth, and invasion when compared to non-target shRNA control cells. Future experiments will focus on determining the importance of Rab27B in TIC exosome production and in vivo tumor growth and metastasis. DISCUSSION/SIGNIFICANCE OF IMPACT: Our results show that Rab27B is important in NSCLC TIC growth and invasion. Further studies are needed to determine the mechanism of Rab27B action. TICs have been linked to enhanced tumorigenic properties, suggesting that Rab27B could be a good candidate for therapeutic targeting of NSCLC TICs.

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