Printer center nanoparticles alter the DNA repair capacity of human bronchial airway epithelial cells

NanoImpact ◽  
2022 ◽  
pp. 100379
Author(s):  
Dimitrios Bitounis ◽  
Qiansheng Huang ◽  
Sneh M. Toprani ◽  
Magdiel I. Setyawati ◽  
Nathalia Oliveira ◽  
...  
Keyword(s):  
Dna Repair ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Repair Capacity ◽  
Dna Repair Capacity

scholarly journals Recent Advances in Molecular Biology of Human Bocavirus 1 and Its Applications

2021 ◽  
Vol 12 ◽  
Author(s):  
Liting Shao ◽  
Weiran Shen ◽  
Shengqi Wang ◽  
Jianming Qiu
Keyword(s):  
Gene Expression ◽  
Dna Repair ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Structural Proteins ◽  
Human Bocavirus ◽  
Airway Epithelial ◽  
Raav Vector ◽  
Human Airway ◽  
Non Coding Rna

Human bocavirus 1 (HBoV1) was discovered in human nasopharyngeal specimens in 2005. It is an autonomous human parvovirus and causes acute respiratory tract infections in young children. HBoV1 infects well differentiated or polarized human airway epithelial cells in vitro. Unique among all parvoviruses, HBoV1 expresses 6 non-structural proteins, NS1, NS1-70, NS2, NS3, NS4, and NP1, and a viral non-coding RNA (BocaSR), and three structural proteins VP1, VP2, and VP3. The BocaSR is the first identified RNA polymerase III (Pol III) transcribed viral non-coding RNA in small DNA viruses. It plays an important role in regulation of viral gene expression and a direct role in viral DNA replication in the nucleus. HBoV1 genome replication in the polarized/non-dividing airway epithelial cells depends on the DNA damage and DNA repair pathways and involves error-free Y-family DNA repair DNA polymerase (Pol) η and Pol κ. Importantly, HBoV1 is a helper virus for the replication of dependoparvovirus, adeno-associated virus (AAV), in polarized human airway epithelial cells, and HBoV1 gene products support wild-type AAV replication and recombinant AAV (rAAV) production in human embryonic kidney (HEK) 293 cells. More importantly, the HBoV1 capsid is able to pseudopackage an rAAV2 or rHBoV1 genome, producing the rAAV2/HBoV1 or rHBoV1 vector. The HBoV1 capsid based rAAV vector has a high tropism for human airway epithelia. A deeper understanding in HBoV1 replication and gene expression will help find a better way to produce the rAAV vector and to increase the efficacy of gene delivery using the rAAV2/HBoV1 or rHBoV1 vector, in particular, to human airways. This review summarizes the recent advances in gene expression and replication of HBoV1, as well as the use of HBoV1 as a parvoviral vector for gene delivery.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial

Differential regulation of the transcriptomic and secretomic landscape of sensor and effector funtions of human airway epithelial cells

Pneumologie ◽  
2018 ◽  
Vol 72 (S 01) ◽  
pp. S100-S101
Author(s):  
R Lehmann ◽  
M Müller ◽  
TE Klassert ◽  
D Driesch ◽  
M Stock ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Differential Regulation ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells
Sign in / Sign up

Export Citation Format

Share Document