Because the existing pulse signal acquisition system cannot measure pulse pressure accurately, this article designs a hardware circuit which adopts AT89C51 as the core to adjust pulse signal condition by acquiring pulse signal with the piezoelectric sensor. The system can filter the noise of signal through wavelet transform. Combining with virtual instrument technology, the circuit can manage and display the pulse waveform smoothly, and finish abnormal alarm and spectrum analysis, etc. A pulse waveform database is set up, which lays the foundation for extracting the waveform characteristics to analyze humans physiological characteristic. Compared with traditional instrument, the new system hardware is based on the development of virtual instrument, which greatly shortens the development cycle and cost, having the characteristics of versatility and ease of development maintenance.