A new biotechnological source of rosmarinic acid and surface flavonoids: Hairy root cultures of Dracocephalum kotschyi Boiss

2013 ◽  
Vol 50 ◽  
pp. 256-263 ◽  
Author(s):  
Mohammad Fattahi ◽  
Vahideh Nazeri ◽  
Laura Torras-Claveria ◽  
Fatemeh Sefidkon ◽  
Rosa M. Cusido ◽  
...  
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Dracocephalum Kotschyi

Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

2007 ◽  
Vol 24 (7) ◽  
pp. 969-972 ◽  
Author(s):  
Sook Young Lee ◽  
Hui Xu ◽  
Yong Kyung Kim ◽  
Sang Un Park
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Agastache Rugosa

scholarly journals The c4h, tat, hppr and hppd Genes Prompted Engineering of Rosmarinic Acid Biosynthetic Pathway in Salvia miltiorrhiza Hairy Root Cultures

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e29713 ◽  
Author(s):  
Ying Xiao ◽  
Lei Zhang ◽  
Shouhong Gao ◽  
Saengking Saechao ◽  
Peng Di ◽  
...  
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Biosynthetic Pathway ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures

scholarly journals Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures

2021 ◽  
Author(s):  
Parvaneh Abrishamchi ◽  
Samaneh Attaran Dowom ◽  
Tayebeh Radjabian ◽  
Seyed Alireza Salami
Keyword(s):  
Methyl Jasmonate ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Hairy Root ◽  
Hairy Roots ◽  
Rosmarinic Acid ◽  
Total Flavonoid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Salvianolic Acid

Abstract Phenolic acids, as the predominant secondary metabolites of Salvia species, are largely used in pharmaceutical industries. The main aim of the study was to establish hairy root cultures of Salvia virgata Jacq. Also, the effects of methyl jasmonate (22.4 and 11.2 ppm), Ag+ ions (5 and 2.5 ppm), and yeast extract (100 and 50 ppm) were assessed on total phenol, total flavonoid, rosmarinic acid, salvianolic acid A, and caffeic acid contents in the hairy roots after 1, 3 and 5 days of exposure. Results showed the used Agrobacterium rhizogenes strains (A4, ATCC15834, R1000, GM1534, and C58C1) differed in their ability to induce hairy roots on leaf explants. The transformed roots were molecularly confirmed using rolC gene and the highest transformation frequency (56 %) was obtained by ATCC 15834 strain. Among the established hairy root lines, the highest amount of rosmarinic acid (0.45 ± 0.01 mg/g DW) and dry root biomass (2.29 ± 0.04 g) was obtained in AT3, the line which was induced by ATCC 15834 strain. The maximum accumulation of total phenol (123.6 ± 0.93 mg GAE/g DW), total flavonoid (5.09 ± 0.07 mg QUE/g DW), rosmarinic acid (18.45 ± 0.8 mg/g DW), salvianolic acid A (2.11 ± 0.04 mg/g DW) and caffeic acid (2.61 ± 0.02 mg/g DW) was observed in the hairy roots elicited with 22.4 ppm methyl jasmonate on day three after treatment. The results support that elicitation could be an effective procedure for the improvement of caffeic acid derivatives production in S. virgata hairy root cultures.

Rosmarinic acid and related phenolics in hairy root cultures of Ocimum basilicum

1996 ◽  
Vol 42 (2) ◽  
pp. 431-434 ◽  
Author(s):  
Hiromi Tada ◽  
Yoshie Murakami ◽  
Toshio Omoto ◽  
Koichiro Shimomura ◽  
Kanji Ishimaru
Keyword(s):  
Hairy Root ◽  
Rosmarinic Acid ◽  
Ocimum Basilicum ◽  
Hairy Root Cultures ◽  
Root Cultures
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