Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant

2015 ◽  
Vol 70 ◽  
pp. 158-162 ◽  
Author(s):  
Ailian Zhang ◽  
Hongzhen Wang ◽  
Qingsong Shao ◽  
Mengjie Xu ◽  
Wangshu Zhang ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Ornamental Plant ◽  
Commercial Application ◽  
Anoectochilus Roxburghii

scholarly journals In Vitro Propagation of Traditional Italian Hazelnut Cultivars as a Tool for the Valorization and Conservation of Local Genetic Resources

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 562-566 ◽  
Author(s):  
Loretta Bacchetta ◽  
Maria Aramini ◽  
Claudia Bernardini ◽  
Eddo Rugini
Keyword(s):  
Genetic Resources ◽  
In Vitro Propagation ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Commercial Application ◽  
Additional Information ◽  
Traditional Cultivars ◽  
Definition Of

The hazelnut (Corylus avellana) is one of the most important crops in the Mediterranean basin. The availability of efficient and reliable in vitro propagation could valorize the local genetic resources. Different studies have been carried out for the definition of an efficient hazelnut micropropagation protocol. These have usually been performed on the most important cultivars, but the application of the micropropagation protocol to the minor ones has produced contradictory results and the technique sometimes had less success than the traditional one. The aim of this work was to gather knowledge and additional information on the in vitro performance of some minor cultivars in comparison with the most used for micropropagation. A revised procedure for the specific medium formulation is suggested. The sterilization and culture establishment phases are discussed in detail. The role of zeatin and 6-benzylamminopurine (BA) in shoot proliferation in the Italian traditional cultivars is compared to improve this phase. The rooting stage proves to be one of the most crucial steps in achieving a large-scale commercial application of hazelnut micropropagation.

In vitro propagation of medicinal and ornamental plant Lysimachia davurica

2017 ◽  
Vol 82 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Yanni Zhang ◽  
◽  
Dongling Sun ◽  
Shangchun Hu ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ornamental Plant

scholarly journals Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Leticia Mascarenhas Pereira Barbosa ◽  
Vespasiano Borges de Paiva Neto ◽  
Leonardo Lucas Carnevalli Dias ◽  
Reginaldo Alves Festucci-Buselli ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

scholarly journals Chemical Characterization of Narcissus poeticus from Sirente –Velino (Apennines - Italy): Galantamine Accumulation and Distribution of Allergenic Compounds in the Flower

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200
Author(s):  
Donatella Ferri ◽  
Carla Ubaldi ◽  
Giordana Marcozzi ◽  
Paolo Fasciani ◽  
Loretta Bacchetta ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Chemical Characterization ◽  
Potential Source ◽  
Flower Stem ◽  
Narcissus Poeticus ◽  
Extraction Solvents ◽  
Plant Flower

Species of Narcissus (family Amaryllidaceae) are a potential source for large-scale extraction of alkaloids and fragrances. The bulbs typically accumulate a large number of alkaloids, including galantamine, a benzazepine alkaloid proven to be a cholinesterase inhibitor and which is used in the treatment of Alzheimer's disease. The presence of galantamine in N. poeticus L. collected in Abruzzo (Italy) was assessed and several levels of alkaloid were found in all parts of the plant (flower, stem, bulb and root) and not only in the bulb. The amount of galantamine obtained was tested by using two different extraction solvents. Extraction of N. poeticus absolute from the flowers was also performed, as this product is an important floral note in perfumery, and the distribution of allergenic compounds in the coronas and in the tepals was assessed. Moreover, the in vitro propagation of N. poeticus was tested as it may be a valuable resource from which to produce biomolecules, as an alternative to chemical synthetic processes.

In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Riaz Rehman ◽  
Muhammad Chaudhary ◽  
Khalid Khawar ◽  
Gang Lu ◽  
Abdul Mannan ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Induction Rate

AbstractPresent study describes rapid in vitro propagation of Caralluma tuberculata, a traditional medicinal plant, and antioxidant potential of calli and plants extracts. The highest callus induction rate (93.3%) with maximum weight of calli 5.2 g was achieved from shoot tip explants on MS medium supplemented with 9.04 μM 2,4-D and 4.44 μM BA. The maximum shoot induction rate (71.1%) with mean number of shoots 3.66 ± 1.53 and 4.6 cm average shoot length was observed on 13.32 μM BA, 4.52 μM 2,4-D and 2.89 μM GA3 appended in MS medium. The developed shoots were best rooted in the presence of 5.07 μM IAA with 3.0 ± 0.15 roots per plantlet. The plants were successfully acclimatized under in vivo conditions. The plants and calli extracts exhibited good antioxidant activities, however, plant extract activities were more pronounced. The phenolic compounds in plant and calli extracts were 0.16% and 0.057%, respectively. While the flavonoids were 0.092% in plant and 0.039% in calli extract. Total Phenolics, flavonoids; DPPH radical scavenging activity and reducing power potential distributed among different fractions depending upon polarity of the solvent. The highest DPPH scavenging activity and reducing power was exhibited by water fractions; 4.95 mg/mL and 0.729 OD at 10 mg/mL, respectively. The micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant. Furthermore, antioxidant value describes importance of this valuable plant as food and medicine.

scholarly journals Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant - Muscari armeniacum Leichtlin. ex Baker

2013 ◽  
Vol 22 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Mustafa Abul Kalam Azad ◽  
Muhammad Nurul Amin
Keyword(s):  
In Vitro Propagation ◽  
Average Length ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Leaf Sheath ◽  
Ornamental Plant ◽  
Garden Soil ◽  
Ex Vitro ◽  
Auxin Concentration

In vitro propagation system has been developed for an important ornamental and medicinal plant, Muscari armeniacum Leichtil. ex Bak. A range of a cytokinin and auxin concentration has been investigated for axillary bulblet proliferation, and direct and indirect adventitious bulblet regeneration from the explants whole bulb, one fourth part of bulb, bulb-scale of ex vitro (field grown mature bulb), and only leaf-sheath explants of in vitro grown bulblet. Axillary bulblet regeneration occurred on MS containing 2.0 - 8.0 ?M BAP or Kn. Direct adventitious bulblets were induced successfully on MS basal medium supplemented with various concentrations of BAP or Kn (1.0 - 4.0 ?M) in combi-nation of either NAA, IBA, or 2,4-D (0.5 - 4.0 ?M). The maximum frequency of adventitious bulblets regeneration occurred from both bulb-scale and leaf-sheath explants on MS with 4.0 ?M BAP and 2.0 ?M NAA, IBA, or 2,4-D. The highest frequency (95.5%) of indirect adventitious bulblets was obtained from in vitro grown leaf-sheathderived callus on MS containing 4.0 ?M BAP with 1.0 ?M 2,4-D whereas, highest number (80.2) and average length (55.5 cm) of bulblets were obtained on MS supplemented with 4.0 ?M BAP and 1.0 ?M NAA. In vitro grown bullets was rooted successfully on MS with 0.5 - 4.0 ?M of IBA, NAA, or IAA. The rooted bulblets were transferred to garden soil and successfully established under ex vitro environment. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14200 Plant Tissue Cult. & Biotech. 22(2): 113-126, 2012 (December)

scholarly journals A REVISED METHOD FOR SUCKER STERILIZATION TO SUPPORT THE IN VITRO PROPAGATION OF SAGO PALM (Metroxylon sagu Rottb.)

2014 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Teuku Tajuddin ◽  
Karyanti . ◽  
Tati Sukarnih ◽  
Nadirman Haska
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Method ◽  
Large Area ◽  
Sago Palm ◽  
Superior Genotypes ◽  
Metroxylon Sagu

Hutan sagu (Metroxylon sagu Rottb.) dapat ditemukan dalam area yang cukup luas di wilayah Maluku dan Papua. Besarnya keanekaragaman hayati dari pohon sagu dapat dilihat di areal ini. Pohon sagu tumbuh secara alami terutama di daerah dataran atau rawa dengan sumber yang air melimpah. Tanaman sagu dapat diperbanyak dengan metode generatif melalui biji, dan vegetatif melalui tunas anakan. Dalam rangka mendukung perbanyakan pohon induk yang unggul secara in vitro dalam skala besar, perbaikan metode sterilisasi tunas anakan mutlak diperlukan. Tunas anakan muda (15-20 cm) yang diperoleh dari Propinsi Papua digunakan sebagai eksplan. Tujuan percobaan sterilisasi ini dilakukan untuk mendukung perbanyakan pohon sagu secara in vitro. Pada percobaan ini antibiotik digunakan untuk membersihkan jaringan internal eksplan dari jamur dan bakteri. Hasil percobaan ini menunjukkan bahwa campuran alkohol dan antibiotik dapat menekan pertumbuhan kontaminan.Kata kunci: Antibiotik, kontaminan jamur dan bakteri, kultur in vitro, metode sterilisasi, sagu ABSTRACTNatural sago (Metroxylon sagu Rottb.) forest can be found in large area in Maluku and Papua regions. There are wide genetic diversities of sago palm found in these areas. This palm grows along riverbanks and in swampy areas which are not suitable for other crops. Sago palm is propagated generatively by seed and vegetatively by suckers. With the purpose of establishing the in vitro culture method for a large-scale of mass clonally propagation of superior genotypes of sago palm, generating sterilized explants are very important. Young suckers (15-20 cm) obtained from areas of Papua Province were used as explants. The sterilization experiments were carrying out to support the tissue culture of sago palm. Sterilization was conducted using antibiotics in order to get rid of fungi and bacteria from inner part of explants tissues. The results showed that from all sterilization methods tested, the best result was treatment using alcohol and antibiotic as disinfectant agents.Keywords: Antibiotics, fungi and bacteria contaminants, in vitro culture, sterilization method, sago palm

scholarly journals Cold tolerance of entomopathogenic nematodes

2009 ◽  
Vol 62 ◽  
pp. 396-396
Author(s):  
F. Ali ◽  
D.A. Wharton
Keyword(s):  
Large Scale ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Freeze Substitution ◽  
Slow Freezing ◽  
Steinernema Feltiae ◽  
Commercial Application ◽  
Subzero Temperature ◽  
Cold Stage

Entomopathogenic nematodes in the Steinernematidae and Heterorhabditidae families are lethal parasites of insects They have great potential as biological control agents since they can be mass produced in vitro However their limited shelf life is the major impediment to their large scale commercial application Attempts to base a storage technology on partial desiccation and anhydrobiosis have met with limited success The natural capacity of entomopathogenic nematodes for freezing tolerance could be an alternative to be exploited as a first step towards developing a method for their longterm storage In the present laboratory experiments the entomopathogenic nematode Steinernema feltiae has shown to be freezetolerant if frozen at relatively high subzero temperature with survival near the level of controls However slow freezing at high subzero temperature (1C) enhanced the nematodes survival at lower temperatures (down to 12C) Freezing of the nematodes was confirmed under the cold stage microscope although the mechanism of survival in the slow freezing regime was difficult to visualise through cold stage microscopy This mechanism needs to be investigated further via a different technique such as freeze substitution and electron microscopy Neither acclimation nor rapid cold hardening improved the survival of nematodes significantly

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