In situ lipase-catalyzed transesterification in rice bran for synthesis of fatty acid methyl ester

2018 ◽  
Vol 120 ◽  
pp. 140-146 ◽  
Author(s):  
Nakyung Choi ◽  
Da Som No ◽  
Heejin Kim ◽  
Byung Hee Kim ◽  
Jieun Kwak ◽  
...  
Fuel ◽  
2010 ◽  
Vol 89 (7) ◽  
pp. 1475-1479 ◽  
Author(s):  
Hong Lei ◽  
Xuefeng Ding ◽  
Hongxi Zhang ◽  
Xue Chen ◽  
Yunling Li ◽  
...  

2014 ◽  
Vol 68 ◽  
pp. 560-569 ◽  
Author(s):  
Vikram Kumar ◽  
Muthusivaramapandian Muthuraj ◽  
Basavaraj Palabhanvi ◽  
Aloke Kumar Ghoshal ◽  
Debasish Das

2021 ◽  
Vol 11 (1) ◽  
pp. 7
Author(s):  
Siriluck Pojjanapornpun ◽  
Apinya Cheewaphan ◽  
Akkaradech Nakornsadet ◽  
Salisa Chumsantea ◽  
Piraporn Sombutsuwan ◽  
...  

The analysis of the fatty acid (FA) profile requires multiple preparation steps, which are lipid extraction followed by derivatization of the FA into a fatty acid methyl ester (FAME). The procedures are time-consuming, and generally require large volumes of sample sizes and solvents. This report proposes a technique for the preparation of FAME from fresh horse mussels without a step of lipid extraction. A rapid in situ derivatization using N,N-dimethylformamide dimethyl acetal (DMF-DMA) methylation followed by alkali-transesterification was examined. In this method, acylglycerols and free fatty acids (medium to long-chain FA) of the sample are targeted to convert into FAME. Direct alkali-transesterification of the fresh sample gave only 58.7% FAME with 12.4% triglyceride and 21.1% FFA. The alkali in situ method showed low conversion efficiency due to the initial sample containing high contents of moisture and FFA (75.11% of the fresh sample and 14.3% of total oil, respectively). The reaction was developed by using two steps in situ derivatization. A 50 mg sample was methylated with 1 mL of DMF-DMA (100 °C, 15 min), followed by transesterified with 10 mL of 1% (w/v) NaOH in methanol (60 °C, 3 min). The conversion into FAME was monitored using size-exclusion HPLC with evaporative light-scattering detection. The column was a 100 Å Phenogel with toluene and 0.25% acetic acid as a mobile phase. The FAME yield of 79.9% with 7.8% triglyceride and 8.5% FFA was obtained. The two steps in situ derivatization gave a promising result with the higher conversion with lower FFA. It is a simple and rapid (less than 20 min) method that requires a low volume of sample and solvent for FAME preparation. However, increasing the conversion efficiency as well as the variety of samples should be further studied.


2014 ◽  
Vol 699 ◽  
pp. 625-631 ◽  
Author(s):  
Nunung Prabaningrum ◽  
Lukman Ismail ◽  
Duvvuri Subbarao

The response surface methodology based on central composite design was applied to optimize three reaction variables including the ratio of methanol volume to seed weight, catalyst concentration, and reaction temperature for conducting in-situ methanolysis of Jatropha curcas seeds. Using RSM, second-order polynomial equations were attained to predict the yield of fatty acid methyl ester. The optimum parameters had been determined which included 8.08 ratio of methanol volume to seed weight, 1.94 wt.% sodium hydroxide concentration, and 56°C reaction temperature. At this optimum condition, the highest biodiesel yield of (90.45 ± 0.25)% was achieved. The yield was similar to the predicted biodiesel yield of 90.98%. The properties of fatty acid methyl ester produced were in agreement with the standards of EN 14214 and ASTM D6751


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