<p>Cellulose enzymes are widely use for biowashing process. It was derived from Aspergillus niger fermentation using rice straw and husk as inoculum medium. In this research, cellulose enzyme was applicated on denim fabric with various enzyme concentration 1%, 2%, 3% owf at 60<sup>o</sup>C and pH 7, liquor ratio 1: 20 for 30 minutes. Characterization of fabric are analized using Scanning Electrone Microscope shows morphological transformation indicate the decrease fabric tensile strenght and stiffness. Denim fabric treatment by 3% cellulose enzyme using rice straw substrate gives morphological and fabric properties characterization similiar with denim fabric treatment by commecial cellulose enzyme.</p>