IL-33 receptor expression on myeloid and plasmacytoid dendritic cells after allergen challenge in patients with allergic rhinitis

Abstract IL-27, as a pleiotropic cytokine, promotes the differentiation of naïve T cells to Th1, while suppressing Th2 and Th17 differentiation in the periphery. However, the role of IL-27 in the thymocyte development remains unknown. Here we showed that IL-27 was highly expressed in thymic plasmacytoid dendritic cells (pDCs) while its receptor expression was mainly detected in CD4+ single-positive (SP) thymocytes. Deletion of the p28 subunit in DCs resulted in a reduction of the most mature Qa-2+ subsets of CD4+ SP T cells. This defect was rescued by intrathymic administration of exogenous IL-27. In vitro differentiation assay further demonstrated that IL-27 alone was able to drive the maturation of the newly generated 6C10+CD69+CD4+ SP cells into Qa-2+ cells. Collectively, this study has revealed an important role of thymic DCs-derived IL-27 in the regulation of the phenotypic maturation of CD4+ SP thymocytes.

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Effects of myeloid and plasmacytoid dendritic cells on ILC2s in patients with allergic rhinitis

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2019.11.029 ◽

2020 ◽

Vol 145 (3) ◽

pp. 855-867.e8 ◽

Cited By ~ 3

Author(s):

Ya-Qi Peng ◽

Zi-Li Qin ◽

Shu-Bin Fang ◽

Zhi-Bin Xu ◽

Hong-Yu Zhang ◽

...

Keyword(s):

Allergic Rhinitis ◽

Dendritic Cells ◽

Plasmacytoid Dendritic Cells

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Role of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression

The Journal of Laryngology & Otology ◽

10.1017/s0022215108003241 ◽

2008 ◽

Vol 122 (12) ◽

pp. 1309-1317 ◽

Cited By ~ 7

Author(s):

T Mackle ◽

S S Gendy ◽

M Walsh ◽

R McConn-Walsh ◽

R W Costello ◽

...

Keyword(s):

Allergic Rhinitis ◽

Flow Measurement ◽

Allergen Challenge ◽

Treated Group ◽

Inspiratory Flow ◽

Receptor Expression ◽

Eosinophil Migration ◽

Before And After ◽

Sphingosine 1 Phosphate ◽

Peak Nasal Inspiratory Flow

AbstractObjective:Recent research has indicated that sphingosine 1-phosphate plays a role in allergy. This study examined the effect of allergen challenge on the expression of sphingosine 1-phosphate receptors on the eosinophils of allergic rhinitis patients, and the effect of steroid treatment on this expression.Study design:A prospective, non-randomised study.Methods:The study had three parts. Firstly, sphingosine 1-phosphate receptor expression on the eosinophils of allergic rhinitis patients and control patients was determined. Secondly, sphingosine 1-phosphate receptor expression was quantified pre- and post-allergen challenge, before and after a short course of fluticasone propionate; all patients underwent symptom scoring and peak nasal inspiratory flow measurement pre- and post-allergen challenge, both before and after steroid or saline treatment. Thirdly, the effect of sphingosine 1-phosphate on eosinophil migration was examined.Results:The eosinophils of both allergic rhinitis patients and controls expressed sphingosine 1-phosphate1, 3, 4, and 5. Eosinophils from all allergic rhinitis patients demonstrated up-regulation in sphingosine 1-phosphate expression after allergen challenge. These changes were statistically very significant for sphingosine 1-phosphate1, 4, and 5, and moderately significant for sphingosine 1-phosphate3. Sphingosine 1-phosphate receptor expression up-regulation was abolished in the steroid-treated group after allergen challenge; however, the saline-treated group showed no change in sphingosine 1-phosphate receptor expression after allergen challenge. Peak nasal inspiratory flow scores were significantly diminished after allergen challenge prior to treatment, but not after a course of topical nasal steroids. Sphingosine 1-phosphate induced eosinophil chemotaxis was increased following allergen challenge in allergic rhinitis subjects.Conclusions:Local intranasal steroid therapy acts directly to block allergen-induced up-regulation of sphingosine 1-phosphate receptors on the peripheral eosinophils of allergic rhinitis patients, and this is coincident with post-challenge peak nasal inspiratory flow measurement improvements. These observations support the idea that such an increase in sphingosine 1-phosphate receptor expression is clinically relevant in allergic rhinitis, with potential consequences for eosinophil migration and survival.

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Role of adenosine receptors in regulating chemotaxis and cytokine production of plasmacytoid dendritic cells

Blood ◽

10.1182/blood-2003-06-1959 ◽

2004 ◽

Vol 103 (4) ◽

pp. 1391-1397 ◽

Cited By ~ 118

Author(s):

Max Schnurr ◽

Tracey Toy ◽

Amanda Shin ◽

Gunther Hartmann ◽

Simon Rothenfusser ◽

...

Keyword(s):

Dendritic Cells ◽

Adenosine Receptors ◽

Plasmacytoid Dendritic Cells ◽

Receptor Expression ◽

Type I ◽

Cpg Oligodeoxynucleotides ◽

A2a Receptor ◽

A1 Receptor ◽

And Function

Abstract Plasmacytoid dendritic cells (PDCs) are potent regulators of immune function and the major source of type I interferon (IFN) following viral infection. PDCs are found at sites of inflammation in allergic reactions, autoimmune disorders, and cancer, but the mechanisms leading to the recruitment of PDCs to these sites remain elusive. During inflammation, adenosine is released and functions as a signaling molecule via adenosine receptors. This study analyzes adenosine receptor expression and function in human PDCs. Adenosine was found to be a potent chemotactic stimulus for immature PDCs via an A1 receptor–mediated mechanism. The migratory response toward adenosine was comparable to that seen with CXCL12 (stromal-derived factor-1α [SDF-1α), the most potent chemotactic stimulus identified thus far for immature PDCs. Upon maturation, PDCs down-regulate the A1 receptor, resulting in a loss of migratory function. In contrast, mature PDCs up-regulate the A2a receptor, which is positively coupled to adenylyl cyclase and has been implicated in the down-regulation of DC cytokine-producing capacity. We show that in mature PDCs adenosine reduces interleukin-6 (IL-6), IL-12, and IFN-α production in response to CpG oligodeoxynucleotides (ODN). These findings indicate that adenosine may play a dual role in PDC-mediated immunity by initially recruiting immature PDCs to sites of inflammation and by subsequently limiting the extent of the inflammatory response induced by mature PDCs by inhibiting their cytokine-producing capacity.

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