scholarly journals Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

iScience ◽  
2021 ◽  
Vol 24 (3) ◽  
pp. 102168 ◽  
Author(s):  
Jiaoqi Gao ◽  
Ning Gao ◽  
Xiaoxin Zhai ◽  
Yongjin J. Zhou
Keyword(s):  
Genome Editing ◽  
Methylotrophic Yeast ◽  
Ogataea Polymorpha

Glucose regulation in the methylotrophic yeast Hansenula (Ogataea) polymorpha is mediated by a putative transceptor Gcr1

2018 ◽  
Vol 103 ◽  
pp. 25-34 ◽  
Author(s):  
Olena G. Stasyk ◽  
Iryna O. Denega ◽  
Dzmitry Padhorny ◽  
Kostyantyn V. Dmytruk ◽  
Dima Kozakov ◽  
...  
Keyword(s):  
Methylotrophic Yeast ◽  
Glucose Regulation ◽  
Ogataea Polymorpha

scholarly journals SIN-like pathway kinases regulate the end of mitosis in the methylotrophic yeast Ogataea polymorpha

2021 ◽  
Author(s):  
Shen Jiangyan ◽  
Kaoru Takegawa ◽  
Gislene Pereira ◽  
Hiromi Maekawa
Keyword(s):  
Budding Yeast ◽  
Methylotrophic Yeast ◽  
Growth Conditions ◽  
Mitotic Exit ◽  
Signalling Pathway ◽  
Significant Delay ◽  
Yeast Saccharomyces Cerevisiae ◽  
Mitotic Exit Network ◽  
Septation Initiation Network ◽  
Ogataea Polymorpha

The Mitotic exit network (MEN) is a conserved signalling pathway essential for termination of mitosis in the budding yeast Saccharomyces cerevisiae. All MEN components are highly conserved in the methylotrophic budding yeast Ogataea polymorpha, except for Cdc15 kinase. Amongst O. polymorpha protein kinases that have some similarity to ScCdc15, only two had no other obvious homologues in S. cerevisiae and these were named OpHCD1 and OpHCD2 for homologue candidate of ScCdc15. A search in other yeast species revealed that OpHcd2 has an armadillo type fold in the C-terminal region as found in SpCdc7 kinases of the fission yeast Schizosaccharomyces pombe, which are homologues of ScCdc15; while OpHcd1 is homologous to SpSid1 kinase, a component of the Septation Initiation Network (SIN) of S. pombe not present in the MEN. Since the deletion of either OpHCD1 or OpHCD2 resulted in lethality under standard growth conditions, conditional mutants were constructed by introducing an ATP analog sensitive mutation. For OpHCD2, we constructed and used new genetic tools for O. polymorpha that combined the Tet promoter and the improved auxin-degron systems. Conditional mutants for OpHCD1 and OpHCD2 exhibited significant delay in late anaphase and defective cell separation, suggesting that both genes have roles in mitotic exit and cytokinesis. These results suggest a SIN-like signalling pathway regulates termination of mitosis in O. polymorpha and that the loss of Sid1/Hcd1 kinase in the MEN occurred relatively recently during the evolution of budding yeast.

Comparative Characteristics of Phytases from Citrobacter freundii and Yersinia intermedia Expressed in Ogataea polymorpha and Pichia pastoris Methylotrophic Yeasts

2019 ◽  
Vol 35 (6) ◽  
pp. 51-56
Author(s):  
M.D. Kashirskaya ◽  
M.N. Lazareva ◽  
A.R. Lapteva ◽  
V.Yu. Dobrynin ◽  
T.L. Gordeeva ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Citrobacter Freundii ◽  
Unique Identifier ◽  
Recombinant Phytase ◽  
The Stability ◽  
First Time ◽  
Yersinia Intermedia ◽  
Ogataea Polymorpha

The genes for bacterial phytases from Citrobacter freundii and Yersinia intermedia were expressed for the first time in a thermotolarant yeast Ogataea polymorpha. A comparative analysis of the properties of recombinant phytases produced by Ogataea polymorpha and Pichia pastoris yeasts was carried out. It was shown that the stability, pH and temperature profiles of the enzyme activities are the same regardless of the host strain. It was proved that O. polymorpha yeast can be used to create producers of feed enzymes and to develop a technology for their cultivation at temperatures above 37 °C. The prospects of using the O. polymorpha yeast for these purposes were evaluated. Ogataea (Hansenula) polymorpha, Pichia pastoris, methylotrophic yeast, thermal tolerance, producer, recombinant phytase The work was financially supported by the Ministry of Science and Higher Education of RF (Project Unique Identifier RFMEFI57917X0145) using the Multipurpose Scientific Installation of All-Russian National Collection of Industrial Microorganisms National Bioresource Center, NRC «Kurchatov Institute»-GosNIIgenetika.

scholarly journals Prooxidant-antioxidant balance in the methylotrophic yeast Ogataea polymorpha exoposed to spermidine

2020 ◽  
Vol 14 (3) ◽  
pp. 13-28
Author(s):  
N. V. Grushanyk ◽  
◽  
I. A. Sarai ◽  
O. V. Stasyk ◽  
O. G. Stasyk ◽  
...  
Keyword(s):  
Methylotrophic Yeast ◽  
Ogataea Polymorpha

scholarly journals Autophagy-related gene ATG13 is involved in control of xylose alcoholic fermentation in the thermotolerant methylotrophic yeast Ogataea polymorpha

2018 ◽  
Vol 18 (2) ◽  
Author(s):  
Kostyantyn V Dmytruk ◽  
Justyna Ruchala ◽  
Dorota Grabek-Lejko ◽  
Czeslaw Puchalski ◽  
Nina V Bulbotka ◽  
...  
Keyword(s):  
Alcoholic Fermentation ◽  
Methylotrophic Yeast ◽  
Related Gene ◽  
Ogataea Polymorpha

scholarly journals GENOMIC DIVERSITY, CHROMOSOMAL REARRANGEMENTS, AND INTERSPECIES HYBRIDIZATION IN THE OGATAEA POLYMORPHA SPECIES COMPLEX

2021 ◽  
Author(s):  
Sara J Hanson ◽  
Eoin Ó Cinnéide ◽  
Letal I Salzberg ◽  
Kenneth H Wolfe ◽  
Jamie McGowan ◽  
...  
Keyword(s):  
Species Complex ◽  
Population Genomics ◽  
Chromosomal Rearrangements ◽  
Nitrate Assimilation ◽  
Methylotrophic Yeast ◽  
Genomic Diversity ◽  
Methanol Metabolism ◽  
Interspecies Hybridization ◽  
Mating Type Switching ◽  
Ogataea Polymorpha

Abstract The methylotrophic yeast Ogataea polymorpha has long been a useful system for recombinant protein production, as well as a model system for methanol metabolism, peroxisome biogenesis, thermotolerance, and nitrate assimilation. It has more recently become an important model for the evolution of mating-type switching. Here, we present a population genomics analysis of 47 isolates within the Ogataea polymorpha species complex, including representatives of the species O. polymorpha, O. parapolymorpha, O. haglerorum, and O. angusta. We found low levels of nucleotide sequence diversity within the O. polymorpha species complex and identified chromosomal rearrangements both within and between species. In addition, we found that one isolate is an interspecies hybrid between O. polymorpha and O. parapolymorpha and present evidence for loss of heterozygosity following hybridization.

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