Reliable mite-specific IgE testing in nasal secretions by means of allergen microarray

Local allergic rhinitis (LAR) is a localized nasal allergic response in the absence of systemic atopy. The symptoms, duration, severity, and comorbidities of LAR are similar to those of allergic rhinitis. Although pathophysiology of LAR is not fully understood, in some patients specific IgE can be demonstrated in the nasal secretions. The diagnosis currently relies on the positive results of nasal provocation test. Nasal provocation test has shown high sensitivity and specificity with safety, and is considered as the gold standard. LAR patients benefit from the same therapeutic strategies as allergic rhinitis patients, including the avoidance of allergen exposure and the pharmacotherapy. Effectiveness and safety of allergen immunotherapy open a window of treatment opportunity in LAR. This review provides a current update on LAR.

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A Photo-immobilized Allergen Microarray for Screening of Allergen-specific IgE

Allergology International ◽

10.2332/allergolint.54.627 ◽

2005 ◽

Vol 54 (4) ◽

pp. 627-631 ◽

Cited By ~ 9

Author(s):

Kunio Ohyama ◽

Kaoru Omura ◽

Yoshihiro Ito

Keyword(s):

Specific Ige ◽

Allergen Microarray

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Intrauterine sensitization of allergen-specific IgE analyzed by a highly sensitive new allergen microarray

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2012.02.023 ◽

2012 ◽

Vol 130 (1) ◽

pp. 113-121.e2 ◽

Cited By ~ 36

Author(s):

Norio Kamemura ◽

Hitomi Tada ◽

Naoki Shimojo ◽

Yoshinori Morita ◽

Yoichi Kohno ◽

...

Keyword(s):

Specific Ige ◽

Highly Sensitive ◽

Allergen Microarray

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Development of allergic rhinitis in early life: A prospective cohort study in high-risk infants

10.22541/au.163251853.35314351/v1 ◽

2021 ◽

Author(s):

Sawako Masuda ◽

Mizuho Nagao ◽

Satoko Usui ◽

Kazutaka Nogami ◽

Yuji Tohda ◽

...

Keyword(s):

Allergic Rhinitis ◽

High Risk ◽

Development Process ◽

Specific Ige ◽

Nasal Symptoms ◽

Nasal Secretions ◽

Nasal Cytology ◽

High Risk Infants ◽

High Risk Cohort

Background: Allergic rhinitis (AR) is the most common allergic disease in children and is closely associated with asthma in the context of atopic march. The development process of AR in early childhood, however, is not well understood due to the absence of definitive diagnostic criteria. We prospectively investigated the process in regard to not only the nasal symptoms and sensitization, but also the nasal cytology, in relation to asthma in a high-risk cohort. Methods: Infants under 2 years of age with atopic dermatitis (AD) and/or food allergy (FA) without a diagnosis of asthma were recruited and followed prospectively for 2 years. The phenotype of perennial AR was classified based on the presence/absence of 1) persistent nasal symptoms, 2) nasal eosinophils and 3) HDM sensitization, the most common allergen for perennial AR in Japan. AR-like phenotypes were defined as positive for at least 2 of those 3 categories. Results: A total of 304 children were enrolled, and 242 subjects (80%) completed the 2-year observation. The prevalence of eosinophilia in nasal secretions increased from 18.5% to 69.9%, while HDM-specific IgE >0.35 kUA/L increased from 30.6% to 74.8%. AR-like phenotypes increased from 18.4% to 65.0%. The cumulative incidence of physician-diagnosed asthma during the 2-year follow-up was significantly higher in the subjects with an AR-like phenotype at 1 year than in those with a non-AR phenotypes. Conclusions: The prevalence of an HDM-related AR-like phenotype was markedly increased during infancy in high-risk infants with AD/FA and was associated with asthma.

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Eosinophilic Esophagitis and Ige-Mediated Allergy in Children: Specific Ige by Component-Based-Allergen Microarray

Journal of Allergy & Therapy ◽

10.4172/2155-6121.1000180 ◽

2014 ◽

Vol 05 (04) ◽

Author(s):

Francesca Rea

Keyword(s):

Eosinophilic Esophagitis ◽

Specific Ige ◽

Ige Mediated ◽

Allergen Microarray

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Nasal Polyps: A Manifestation of Allergy?

Otolaryngology ◽

10.1177/019459988910100605 ◽

1989 ◽

Vol 101 (6) ◽

pp. 641-645 ◽

Cited By ~ 20

Author(s):

Jonathan A. Perkins ◽

Don B. Blakeslee ◽

Pierre Andrade

Keyword(s):

Allergic Disease ◽

Nasal Polyps ◽

Nasal Polyposis ◽

Skin Testing ◽

Specific Ige ◽

Similar Data ◽

Control Groups ◽

Nasal Disease ◽

Nasal Secretions ◽

Ige Determination

The cause of nasal polyps is speculative. In the 1930s nasal polyps were considered to be a manifestation of allergy. This theory was challenged in the 1970s. Many patients with nasal polyps have no Identifiable allergic disease. The purpose of this study is to better define the relationship between allergy and nasal polyps. Twelve patients with nasal polyps were evaluated by history, physical examination, skin testing, nasal smear for eosinophils, specific IgE determination from serum, nasal secretions, and nasal polyp fluid. Similar data were collected from three control groups: (1) 10 patients with allergic rhinitis, (2) 10 patients with nonallelic rhinitis, and (3) 10 patients with no known nasal disease. By comparing the Incidence of markers for allergic disease In patients with nasal polyposis to each of the three control groups, the role of allergic disease In nasal polyposis was delineated.

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in NS

10.22541/au.163736696.65238914/v1 ◽

2021 ◽

Author(s):

Stefania Arasi ◽

Ilenia Panasiti ◽

Lucia Caminiti ◽

Mariaelisabetta Conte ◽

Sveva Castelli ◽

...

Keyword(s):

Late Phase ◽

Specific Treatment ◽

Allergic Inflammation ◽

Serum Levels ◽

Specific Ige ◽

Neurotrophin 3 ◽

Paediatric Age ◽

Nasal Secretions ◽

Tailored Approach

BACKGROUND: Characterization of disease endotypes will open a new window for the treatment of allergic rhinitis (AR). Herein we provide the first attempt to identify specific AR phenotypes/endotypes and/or any biomarker/predictor for specific treatment response based on local biological parameters. METHODS: This observational study was carried out in 142 patients with seasonal AR and 20 non-allergic controls. Total IgE levels, specific IgE to 112 allergenic molecules and 92 proinflammatory and immunologic proteins were measured in both serum and nasal secretions (NS). RESULTS: We found increased values of MCPs and MMPs in adults both in NS and serum when compared with pediatric patients (p<.05). MCPs and MMPs might represent two effective predictors of chronic inflammation. CXCL9, CXCL10, CXCL11, MCPs and MMP1 showed an upward trend both in serum and NS for patients with ≥ 3 comorbidities vs non-allergic controls(p<.05). These data suggest the involvement of these chemokines in the late phase of chronic allergic inflammation in the nose. Serum levels of IL-6, IL-8 and IL-10 (p<.05) were significantly higher in patients with AR+asthma compared to patients with different comorbidities. Conversely, serum levels of neurotrophin-3 values (p<.05) were significantly higher in those with AR+eczema vs other comorbidities groups. A subgroup of patients with a nasal hypersecretory state,called “hypersecreter endotype” was characterized by paediatric age, male gender, grass pollen sensitization and distributed among persistent, mild or moderate to severe cases of AR. CONCLUSIONS: Our study sets the groundwork for an AR endotypization at molecular level, which is highly desirable to deliver a patient-tailored approach.

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