Production of non-embryogenic synthetic seeds for propagation and germplasm transfer of Hedychium coronarium J. Koenig

Auxin plays a key role in different plant growth and development processes, including flower opening and development. The perception and signaling of auxin depend on the cooperative action of various components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play an imperative role. In a recent study, the entire Aux/IAA gene family was identified and comprehensively analyzed in Hedychium coronarium, a scented species used as an ornamental plant for cut flowers. Phylogenetic analysis showed that the Aux/IAA gene family in H. coronarium is slightly contracted compared to Arabidopsis, with low levels of non-canonical proteins. Sequence analysis of promoters showed numerous cis-regulatory elements related to various phytohormones. HcIAA genes showed distinct expression patterns in different tissues and flower developmental stages, and some HcIAA genes showed significant responses to auxin and ethylene, indicating that Aux/IAAs may play an important role in linking hormone signaling pathways. Based on the expression profiles, HcIAA2, HcIAA4, HcIAA6 and HcIAA12, were selected as candidate genes and HcIAA2 and HcIAA4 were screened for further characterization. Downregulation of HcIAA2 and HcIAA4 by virus-induced gene silencing in H. coronarium flowers modified the total volatile compound content, suggesting that HcIAA2 and HcIAA4 play important roles in H. coronarium floral scent formation. The results presented here will provide insights into the putative roles of HcIAA genes and will assist the elucidation of their precise roles during floral scent formation.

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CULTURAL SYSTEM PRODUCING ENCAPSULATABLE UNITS OF SYNTHETIC SEEDS IN CELERY

Acta Horticulturae ◽

10.17660/actahortic.1992.319.12 ◽

1992 ◽

pp. 113-118 ◽

Cited By ~ 8

Author(s):

N. Onishi ◽

T. Mashiko ◽

A. Okamoto

Keyword(s):

Synthetic Seeds ◽

Cultural System

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Synthetic Seeds: A Valuable Adjunct for Conservation of Medicinal Plants

Synthetic Seeds ◽

10.1007/978-3-030-24631-0_7 ◽

2019 ◽

pp. 181-216 ◽

Cited By ~ 1

Author(s):

Neelam Sharma ◽

R. Gowthami ◽

Ruchira Pandey

Keyword(s):

Medicinal Plants ◽

Synthetic Seeds ◽

Valuable Adjunct

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Synthetic Seeds: Relevance to Endangered Germplasm Conservation In Vitro

Synthetic Seeds ◽

10.1007/978-3-030-24631-0_2 ◽

2019 ◽

pp. 21-60 ◽

Cited By ~ 1

Author(s):

Akansha Saxena ◽

Mukund Shukla ◽

Praveen Saxena

Keyword(s):

Germplasm Conservation ◽

Synthetic Seeds

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Hedychium coronarium J. Koenig

Natural Compounds ◽

10.1007/978-1-4614-0539-9_457 ◽

2013 ◽

pp. 147-147

Keyword(s):

Hedychium Coronarium

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Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v23i2.17506 ◽

2014 ◽

Vol 23 (2) ◽

pp. 147-155 ◽

Cited By ~ 1

Author(s):

Dinesh Giri ◽

Sushma Tamta

Keyword(s):

Somatic Embryogenesis ◽

Medicinal Plant ◽

Somatic Embryos ◽

Synthetic Seeds ◽

Ex Situ ◽

Secondary Embryogenesis ◽

Zygotic Embryos ◽

High Concentration ◽

Cotyledon Explants ◽

Short Period

This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013 (December)

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Contribuição ao estudo das plantas medicinais no Estado de Alagoas V

Acta Amazonica ◽

10.1590/1809-43921988185312 ◽

1988 ◽

Vol 18 (suppl 1-2) ◽

pp. 305-312 ◽

Cited By ~ 1

Author(s):

Cornélio Ramalho Campelo

Keyword(s):

Daucus Carota ◽

Jatropha Curcas ◽

Eucalyptus Citriodora ◽

Daucus Carota L ◽

Jatropha Curcas L ◽

Hedychium Coronarium ◽

Guazuma Ulmifolia ◽

Polygonum Persicaria

O nosso trabalho é mais uma etapa, a quinta, do estudo sobre as plantas medicinais, ocorrentes no Estado de Alagoas. Várias excursões foram feitas, a fim de coletar espécies, utilizadas na medicina caseira. Os espécimens coletados foram preparados, identificados e incorporados ao Herbario "Professor Honorio Monteiro" (MUFAL) da Universidade Federal de Alagoas. O estudo das plantas medicinais, abrangendo famílias, nomes científicos e vulgares e a descrição sucinta para cada espécie, bem como o uso medicinal e a posologia, foi feito com auxílio de bibliografias especializadas no assunto. Foram estudadas as seguintes espécies, consideradas medicinais: Allium ascalonicum L. (cebola-branca); Astronium urundeuva (Fr. All.) Engl. (aroeira-do-sertão); Cecropia sp. (imbáuba); Coix lacryma-jobi L. (capim-de-contas); Daucus carota L. (cenoura); Eucalyptus citriodora Hook. (eucalípto); Eugenia jambosa L. (jambo-rosa); Eugenia malaccensis L. (jambo-roxo); Genipa americana L. (genipapo); Guazuma ulmifolia Lam. (mutamba); Helianthus annuusL. (girassol); Hedychium coronarium Koening (lírio-do-brejo); Imperata brasiliensis Trin. (capim-sapê); Jatropha curcas L. (pinhão-manso) Melocactus bahiensis (Brit. et Rose) Luetzelb. (coroa-de-frade), Monniera trifolia L. (alfavaca-de-cobra), Pithecollobium avaremotemo Mart. (bordão-de-velho); Polygonum persicaria L. (erva-de-bicho); Solidago microglossa DC. (erva-lanceta) e Syzygium jambolana DC. (azeitona).

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