HPV RNA in situ Hybridization in HPV-Related Head and Neck Squamous Cell Carcinoma: An Improvement over HPV DNA in situ Hybridization

2018 ◽  
Vol 7 (5) ◽  
pp. S12-S13
Author(s):  
James Miller ◽  
Zahra Maleki
Keyword(s):  
Squamous Cell Carcinoma ◽  
In Situ Hybridization ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv Dna ◽  
Rna In Situ Hybridization

Use of In Situ Hybridization to Detect Human Papillomavirus in Head and Neck Squamous Cell Carcinoma Patients Without a History of Alcohol or Tobacco Use

2008 ◽  
Vol 132 (10) ◽  
pp. 1653-1656
Author(s):  
Walter T. Lee ◽  
Raymond R. Tubbs ◽  
Aysenur M. Teker ◽  
Joseph Scharpf ◽  
Marshall Strome ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
In Situ Hybridization ◽  
Alcohol Use ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
History Of ◽  
High Risk Hpv

Abstract Context.—Head and neck squamous cell carcinoma is commonly associated with tobacco and alcohol use. There are, however, a group of patients without a significant history of tobacco or alcohol use, and the etiology of these tumors is incompletely understood. Objective.—To examine tumors in this subpopulation for association with human papillomavirus (HPV) using newly available in situ hybridization probes. Design.—Between October 2004 and October 2005, 22 patients who did not use alcohol or tobacco were included. Formalin-fixed, paraffin-embedded tissue sections were used to perform in situ hybridization using newly available probe sets (Ventana Medical Systems, Tucson, Ariz). The slides were examined for the presence of integrated HPV using light microscopy. Positive and negative xenograft controls were run with the assay. Results.—The mean age of the patients was 64 years. There were 14 men and 8 women. The most common anatomic sites included tongue (n = 8), tonsil (n = 7), and larynx (n = 7). All cases and controls were successfully stained. Only 2 cases were positive for high-risk HPV, and both demonstrated an integrated pattern. Both cases were tumors of the tonsil. No cases were positive for low-risk HPV. Conclusions.—These results demonstrate that the new probe sets for HPV can be used very efficiently in clinical pathology material of head and neck squamous cell carcinoma. Our data show that high-risk HPV is an uncommon finding in head and neck squamous cell carcinoma from patients who do not have a history of tobacco or alcohol use; low-risk HPV was not seen in any case.

Fluorescence in situ hybridization for detecting TP16MTS1/CDK41 gene deletions in squamous cell carcinoma of the head and neck

2003 ◽  
Vol 141 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Ali Namazie ◽  
Sassan Alavi ◽  
Neema Aghamohammadi ◽  
Mazda Aghamohammadi ◽  
Thomas C Calcaterra ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Gene Deletions

scholarly journals Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

2020 ◽  
Vol 14 (1) ◽  
pp. 57-64
Author(s):  
Ashraf I. Khasawneh ◽  
Nisreen Himsawi ◽  
Jumana Abu-Raideh ◽  
Muna Salameh ◽  
Niveen Abdullah ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Prophylactic Measures

Background: In addition to smoking and alcohol consumption, human papillomavirus (HPV) is a leading etiology for Head and Neck Squamous Cell Carcinoma (HNSCC). However, this causal association is still understudied in Middle Eastern populations. Objective: The aim of this study was to determine the prevalence of HPV-associated infection in the Jordanian HNSCC patients and the associated HPV genotypes. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) squamous cell carcinoma samples of the head and neck were collected from two referral centers in Amman, Jordan to determine the existence of HPV DNA. After DNA extraction HPV infection and genotyping were identified using real-time PCR. Results: HPV DNA was detected in 19 out of 61 (31.1%) HNSCC samples. Despite screening for 28 different genotypes, HPV 16 was the only genotype identified in all examined samples. Most HPV-positive samples were obtained from the oropharynx (41.7%), oral cavity (37%), and larynx (18.2%). No significant association between HPV 16 genotype and age, sex, tobacco use, anatomical location, or tumor grade was noticed. Conclusion: This study reported a high association between HPV 16 genotype and HNSCC in Jordanian patients. These data should facilitate the implementation of appropriate HPV awareness campaigns, and activate selective prophylactic measures against HPV infection.

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